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Lung ultrasound is a reliable method for evaluating extravascular lung water volume in rodents
BACKGROUD: Lung ultrasound (LUS) can diagnose extravacular lung water (EVLW) through the visualization of B lines in both humans and large animals. However, there are no published data on the use of ultrasound to detect EVLW in rats, the gold standard to evaluate of EVLW in rats is post-mortem gravi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4643534/ https://www.ncbi.nlm.nih.gov/pubmed/26563098 http://dx.doi.org/10.1186/s12871-015-0146-1 |
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author | Ma, Huan Huang, Daozheng Zhang, Minzhou Huang, Xin Ma, Shiyu Mao, Shuai Li, Wenhui Chen, Yanfen Guo, Liheng |
author_facet | Ma, Huan Huang, Daozheng Zhang, Minzhou Huang, Xin Ma, Shiyu Mao, Shuai Li, Wenhui Chen, Yanfen Guo, Liheng |
author_sort | Ma, Huan |
collection | PubMed |
description | BACKGROUD: Lung ultrasound (LUS) can diagnose extravacular lung water (EVLW) through the visualization of B lines in both humans and large animals. However, there are no published data on the use of ultrasound to detect EVLW in rats, the gold standard to evaluate of EVLW in rats is post-mortem gravimetric analysis. The present study was designed to determine the similarity between lung sonography and gravimetric measurements of EVLW in rats in an acute lung injury (ALI) model. METHODS: Thirty male Sprague–Dawley rats were randomized into control and experimental groups. The B lines were measured byLUS at baseline. ALI was induced by the intravenous administration of oleic acid (OA) at a dose of 9 ul/100 mg, and controls were injected the same amount of isotonic saline. After 1 h, B-lines were measured by LUS in each rat following the induction of ALI. At the end of each experiment, both lungs were dissected, weighed and dried to determine wet/dry weight ratio according to the standard gravimetric methodology. Lung samples from three rats in each group were examined histologically. RESULTS: B-lines were present in all rats from experimental group at 1 h point after OA injection. The statistical correlation between the two methods of assessing EVLW provided an r = 0.834 (p < 0.001). Repeatability studies of the LUS technique (Bland-Altman plots) showed good intra-observer and inter-observer reproducibility. CONCLUSION: The data suggest that, in an experimental rat model of ALI, B lines score as assessed by LUS can provide an easy, semi-quantitative, noninvasive. Real-time index of EVLW which is strongly correlated to experimental gravimetric assessments. |
format | Online Article Text |
id | pubmed-4643534 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-46435342015-11-14 Lung ultrasound is a reliable method for evaluating extravascular lung water volume in rodents Ma, Huan Huang, Daozheng Zhang, Minzhou Huang, Xin Ma, Shiyu Mao, Shuai Li, Wenhui Chen, Yanfen Guo, Liheng BMC Anesthesiol Research Article BACKGROUD: Lung ultrasound (LUS) can diagnose extravacular lung water (EVLW) through the visualization of B lines in both humans and large animals. However, there are no published data on the use of ultrasound to detect EVLW in rats, the gold standard to evaluate of EVLW in rats is post-mortem gravimetric analysis. The present study was designed to determine the similarity between lung sonography and gravimetric measurements of EVLW in rats in an acute lung injury (ALI) model. METHODS: Thirty male Sprague–Dawley rats were randomized into control and experimental groups. The B lines were measured byLUS at baseline. ALI was induced by the intravenous administration of oleic acid (OA) at a dose of 9 ul/100 mg, and controls were injected the same amount of isotonic saline. After 1 h, B-lines were measured by LUS in each rat following the induction of ALI. At the end of each experiment, both lungs were dissected, weighed and dried to determine wet/dry weight ratio according to the standard gravimetric methodology. Lung samples from three rats in each group were examined histologically. RESULTS: B-lines were present in all rats from experimental group at 1 h point after OA injection. The statistical correlation between the two methods of assessing EVLW provided an r = 0.834 (p < 0.001). Repeatability studies of the LUS technique (Bland-Altman plots) showed good intra-observer and inter-observer reproducibility. CONCLUSION: The data suggest that, in an experimental rat model of ALI, B lines score as assessed by LUS can provide an easy, semi-quantitative, noninvasive. Real-time index of EVLW which is strongly correlated to experimental gravimetric assessments. BioMed Central 2015-11-12 /pmc/articles/PMC4643534/ /pubmed/26563098 http://dx.doi.org/10.1186/s12871-015-0146-1 Text en © Ma et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Ma, Huan Huang, Daozheng Zhang, Minzhou Huang, Xin Ma, Shiyu Mao, Shuai Li, Wenhui Chen, Yanfen Guo, Liheng Lung ultrasound is a reliable method for evaluating extravascular lung water volume in rodents |
title | Lung ultrasound is a reliable method for evaluating extravascular lung water volume in rodents |
title_full | Lung ultrasound is a reliable method for evaluating extravascular lung water volume in rodents |
title_fullStr | Lung ultrasound is a reliable method for evaluating extravascular lung water volume in rodents |
title_full_unstemmed | Lung ultrasound is a reliable method for evaluating extravascular lung water volume in rodents |
title_short | Lung ultrasound is a reliable method for evaluating extravascular lung water volume in rodents |
title_sort | lung ultrasound is a reliable method for evaluating extravascular lung water volume in rodents |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4643534/ https://www.ncbi.nlm.nih.gov/pubmed/26563098 http://dx.doi.org/10.1186/s12871-015-0146-1 |
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