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CRISPR-Cas9 delivery to hard-to-transfect cells via membrane deformation
The CRISPR (clustered regularly interspaced short palindromic repeats)–Cas (CRISPR-associated) nuclease system represents an efficient tool for genome editing and gene function analysis. It consists of two components: single-guide RNA (sgRNA) and the enzyme Cas9. Typical sgRNA and Cas9 intracellular...
Autores principales: | Han, Xin, Liu, Zongbin, Jo, Myeong chan, Zhang, Kai, Li, Ying, Zeng, Zihua, Li, Nan, Zu, Youli, Qin, Lidong |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4643799/ https://www.ncbi.nlm.nih.gov/pubmed/26601238 http://dx.doi.org/10.1126/sciadv.1500454 |
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