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LPS-Stimulated Human Skin-Derived Stem Cells Enhance Neo-Vascularization during Dermal Regeneration
High numbers of adult stem cells are still required to improve the formation of new vessels in scaffolds to accelerate dermal regeneration. Recent data indicate a benefit for vascularization capacity by stimulating stem cells with lipopolysaccharide (LPS). In this study, stem cells derived from huma...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4643997/ https://www.ncbi.nlm.nih.gov/pubmed/26565617 http://dx.doi.org/10.1371/journal.pone.0142907 |
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author | Kisch, Tobias Weber, Caroline Rapoport, Daniel H. Kruse, Charli Schumann, Sandra Stang, Felix H. Siemers, Frank Matthießen, Anna E. |
author_facet | Kisch, Tobias Weber, Caroline Rapoport, Daniel H. Kruse, Charli Schumann, Sandra Stang, Felix H. Siemers, Frank Matthießen, Anna E. |
author_sort | Kisch, Tobias |
collection | PubMed |
description | High numbers of adult stem cells are still required to improve the formation of new vessels in scaffolds to accelerate dermal regeneration. Recent data indicate a benefit for vascularization capacity by stimulating stem cells with lipopolysaccharide (LPS). In this study, stem cells derived from human skin (SDSC) were activated with LPS and seeded in a commercially available dermal substitute to examine vascularization in vivo. Besides, in vitro assays were performed to evaluate angiogenic factor release and tube formation ability. Results showed that LPS-activated SDSC significantly enhanced vascularization of the scaffolds, compared to unstimulated stem cells in vivo. Further, in vitro assays confirmed higher secretion rates of proangiogenic as well as proinflammatoric factors in the presence of LPS-activated SDSC. Our results suggest that combining activated stem cells and a dermal substitute is a promising option to enhance vascularization in scaffold-mediated dermal regeneration. |
format | Online Article Text |
id | pubmed-4643997 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-46439972015-11-18 LPS-Stimulated Human Skin-Derived Stem Cells Enhance Neo-Vascularization during Dermal Regeneration Kisch, Tobias Weber, Caroline Rapoport, Daniel H. Kruse, Charli Schumann, Sandra Stang, Felix H. Siemers, Frank Matthießen, Anna E. PLoS One Research Article High numbers of adult stem cells are still required to improve the formation of new vessels in scaffolds to accelerate dermal regeneration. Recent data indicate a benefit for vascularization capacity by stimulating stem cells with lipopolysaccharide (LPS). In this study, stem cells derived from human skin (SDSC) were activated with LPS and seeded in a commercially available dermal substitute to examine vascularization in vivo. Besides, in vitro assays were performed to evaluate angiogenic factor release and tube formation ability. Results showed that LPS-activated SDSC significantly enhanced vascularization of the scaffolds, compared to unstimulated stem cells in vivo. Further, in vitro assays confirmed higher secretion rates of proangiogenic as well as proinflammatoric factors in the presence of LPS-activated SDSC. Our results suggest that combining activated stem cells and a dermal substitute is a promising option to enhance vascularization in scaffold-mediated dermal regeneration. Public Library of Science 2015-11-13 /pmc/articles/PMC4643997/ /pubmed/26565617 http://dx.doi.org/10.1371/journal.pone.0142907 Text en © 2015 Kisch et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Kisch, Tobias Weber, Caroline Rapoport, Daniel H. Kruse, Charli Schumann, Sandra Stang, Felix H. Siemers, Frank Matthießen, Anna E. LPS-Stimulated Human Skin-Derived Stem Cells Enhance Neo-Vascularization during Dermal Regeneration |
title | LPS-Stimulated Human Skin-Derived Stem Cells Enhance Neo-Vascularization during Dermal Regeneration |
title_full | LPS-Stimulated Human Skin-Derived Stem Cells Enhance Neo-Vascularization during Dermal Regeneration |
title_fullStr | LPS-Stimulated Human Skin-Derived Stem Cells Enhance Neo-Vascularization during Dermal Regeneration |
title_full_unstemmed | LPS-Stimulated Human Skin-Derived Stem Cells Enhance Neo-Vascularization during Dermal Regeneration |
title_short | LPS-Stimulated Human Skin-Derived Stem Cells Enhance Neo-Vascularization during Dermal Regeneration |
title_sort | lps-stimulated human skin-derived stem cells enhance neo-vascularization during dermal regeneration |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4643997/ https://www.ncbi.nlm.nih.gov/pubmed/26565617 http://dx.doi.org/10.1371/journal.pone.0142907 |
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