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RNA-Seq of Tumor-Educated Platelets Enables Blood-Based Pan-Cancer, Multiclass, and Molecular Pathway Cancer Diagnostics
Tumor-educated blood platelets (TEPs) are implicated as central players in the systemic and local responses to tumor growth, thereby altering their RNA profile. We determined the diagnostic potential of TEPs by mRNA sequencing of 283 platelet samples. We distinguished 228 patients with localized and...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Cell Press
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4644263/ https://www.ncbi.nlm.nih.gov/pubmed/26525104 http://dx.doi.org/10.1016/j.ccell.2015.09.018 |
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| author | Best, Myron G. Sol, Nik Kooi, Irsan Tannous, Jihane Westerman, Bart A. Rustenburg, François Schellen, Pepijn Verschueren, Heleen Post, Edward Koster, Jan Ylstra, Bauke Ameziane, Najim Dorsman, Josephine Smit, Egbert F. Verheul, Henk M. Noske, David P. Reijneveld, Jaap C. Nilsson, R. Jonas A. Tannous, Bakhos A. Wesseling, Pieter Wurdinger, Thomas |
| author_facet | Best, Myron G. Sol, Nik Kooi, Irsan Tannous, Jihane Westerman, Bart A. Rustenburg, François Schellen, Pepijn Verschueren, Heleen Post, Edward Koster, Jan Ylstra, Bauke Ameziane, Najim Dorsman, Josephine Smit, Egbert F. Verheul, Henk M. Noske, David P. Reijneveld, Jaap C. Nilsson, R. Jonas A. Tannous, Bakhos A. Wesseling, Pieter Wurdinger, Thomas |
| author_sort | Best, Myron G. |
| collection | PubMed |
| description | Tumor-educated blood platelets (TEPs) are implicated as central players in the systemic and local responses to tumor growth, thereby altering their RNA profile. We determined the diagnostic potential of TEPs by mRNA sequencing of 283 platelet samples. We distinguished 228 patients with localized and metastasized tumors from 55 healthy individuals with 96% accuracy. Across six different tumor types, the location of the primary tumor was correctly identified with 71% accuracy. Also, MET or HER2-positive, and mutant KRAS, EGFR, or PIK3CA tumors were accurately distinguished using surrogate TEP mRNA profiles. Our results indicate that blood platelets provide a valuable platform for pan-cancer, multiclass cancer, and companion diagnostics, possibly enabling clinical advances in blood-based “liquid biopsies”. |
| format | Online Article Text |
| id | pubmed-4644263 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2015 |
| publisher | Cell Press |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-46442632015-12-08 RNA-Seq of Tumor-Educated Platelets Enables Blood-Based Pan-Cancer, Multiclass, and Molecular Pathway Cancer Diagnostics Best, Myron G. Sol, Nik Kooi, Irsan Tannous, Jihane Westerman, Bart A. Rustenburg, François Schellen, Pepijn Verschueren, Heleen Post, Edward Koster, Jan Ylstra, Bauke Ameziane, Najim Dorsman, Josephine Smit, Egbert F. Verheul, Henk M. Noske, David P. Reijneveld, Jaap C. Nilsson, R. Jonas A. Tannous, Bakhos A. Wesseling, Pieter Wurdinger, Thomas Cancer Cell Article Tumor-educated blood platelets (TEPs) are implicated as central players in the systemic and local responses to tumor growth, thereby altering their RNA profile. We determined the diagnostic potential of TEPs by mRNA sequencing of 283 platelet samples. We distinguished 228 patients with localized and metastasized tumors from 55 healthy individuals with 96% accuracy. Across six different tumor types, the location of the primary tumor was correctly identified with 71% accuracy. Also, MET or HER2-positive, and mutant KRAS, EGFR, or PIK3CA tumors were accurately distinguished using surrogate TEP mRNA profiles. Our results indicate that blood platelets provide a valuable platform for pan-cancer, multiclass cancer, and companion diagnostics, possibly enabling clinical advances in blood-based “liquid biopsies”. Cell Press 2015-11-09 /pmc/articles/PMC4644263/ /pubmed/26525104 http://dx.doi.org/10.1016/j.ccell.2015.09.018 Text en © 2015 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| spellingShingle | Article Best, Myron G. Sol, Nik Kooi, Irsan Tannous, Jihane Westerman, Bart A. Rustenburg, François Schellen, Pepijn Verschueren, Heleen Post, Edward Koster, Jan Ylstra, Bauke Ameziane, Najim Dorsman, Josephine Smit, Egbert F. Verheul, Henk M. Noske, David P. Reijneveld, Jaap C. Nilsson, R. Jonas A. Tannous, Bakhos A. Wesseling, Pieter Wurdinger, Thomas RNA-Seq of Tumor-Educated Platelets Enables Blood-Based Pan-Cancer, Multiclass, and Molecular Pathway Cancer Diagnostics |
| title | RNA-Seq of Tumor-Educated Platelets Enables Blood-Based Pan-Cancer, Multiclass, and Molecular Pathway Cancer Diagnostics |
| title_full | RNA-Seq of Tumor-Educated Platelets Enables Blood-Based Pan-Cancer, Multiclass, and Molecular Pathway Cancer Diagnostics |
| title_fullStr | RNA-Seq of Tumor-Educated Platelets Enables Blood-Based Pan-Cancer, Multiclass, and Molecular Pathway Cancer Diagnostics |
| title_full_unstemmed | RNA-Seq of Tumor-Educated Platelets Enables Blood-Based Pan-Cancer, Multiclass, and Molecular Pathway Cancer Diagnostics |
| title_short | RNA-Seq of Tumor-Educated Platelets Enables Blood-Based Pan-Cancer, Multiclass, and Molecular Pathway Cancer Diagnostics |
| title_sort | rna-seq of tumor-educated platelets enables blood-based pan-cancer, multiclass, and molecular pathway cancer diagnostics |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4644263/ https://www.ncbi.nlm.nih.gov/pubmed/26525104 http://dx.doi.org/10.1016/j.ccell.2015.09.018 |
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