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RNA-Seq of Tumor-Educated Platelets Enables Blood-Based Pan-Cancer, Multiclass, and Molecular Pathway Cancer Diagnostics

Tumor-educated blood platelets (TEPs) are implicated as central players in the systemic and local responses to tumor growth, thereby altering their RNA profile. We determined the diagnostic potential of TEPs by mRNA sequencing of 283 platelet samples. We distinguished 228 patients with localized and...

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Autores principales: Best, Myron G., Sol, Nik, Kooi, Irsan, Tannous, Jihane, Westerman, Bart A., Rustenburg, François, Schellen, Pepijn, Verschueren, Heleen, Post, Edward, Koster, Jan, Ylstra, Bauke, Ameziane, Najim, Dorsman, Josephine, Smit, Egbert F., Verheul, Henk M., Noske, David P., Reijneveld, Jaap C., Nilsson, R. Jonas A., Tannous, Bakhos A., Wesseling, Pieter, Wurdinger, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4644263/
https://www.ncbi.nlm.nih.gov/pubmed/26525104
http://dx.doi.org/10.1016/j.ccell.2015.09.018
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author Best, Myron G.
Sol, Nik
Kooi, Irsan
Tannous, Jihane
Westerman, Bart A.
Rustenburg, François
Schellen, Pepijn
Verschueren, Heleen
Post, Edward
Koster, Jan
Ylstra, Bauke
Ameziane, Najim
Dorsman, Josephine
Smit, Egbert F.
Verheul, Henk M.
Noske, David P.
Reijneveld, Jaap C.
Nilsson, R. Jonas A.
Tannous, Bakhos A.
Wesseling, Pieter
Wurdinger, Thomas
author_facet Best, Myron G.
Sol, Nik
Kooi, Irsan
Tannous, Jihane
Westerman, Bart A.
Rustenburg, François
Schellen, Pepijn
Verschueren, Heleen
Post, Edward
Koster, Jan
Ylstra, Bauke
Ameziane, Najim
Dorsman, Josephine
Smit, Egbert F.
Verheul, Henk M.
Noske, David P.
Reijneveld, Jaap C.
Nilsson, R. Jonas A.
Tannous, Bakhos A.
Wesseling, Pieter
Wurdinger, Thomas
author_sort Best, Myron G.
collection PubMed
description Tumor-educated blood platelets (TEPs) are implicated as central players in the systemic and local responses to tumor growth, thereby altering their RNA profile. We determined the diagnostic potential of TEPs by mRNA sequencing of 283 platelet samples. We distinguished 228 patients with localized and metastasized tumors from 55 healthy individuals with 96% accuracy. Across six different tumor types, the location of the primary tumor was correctly identified with 71% accuracy. Also, MET or HER2-positive, and mutant KRAS, EGFR, or PIK3CA tumors were accurately distinguished using surrogate TEP mRNA profiles. Our results indicate that blood platelets provide a valuable platform for pan-cancer, multiclass cancer, and companion diagnostics, possibly enabling clinical advances in blood-based “liquid biopsies”.
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spelling pubmed-46442632015-12-08 RNA-Seq of Tumor-Educated Platelets Enables Blood-Based Pan-Cancer, Multiclass, and Molecular Pathway Cancer Diagnostics Best, Myron G. Sol, Nik Kooi, Irsan Tannous, Jihane Westerman, Bart A. Rustenburg, François Schellen, Pepijn Verschueren, Heleen Post, Edward Koster, Jan Ylstra, Bauke Ameziane, Najim Dorsman, Josephine Smit, Egbert F. Verheul, Henk M. Noske, David P. Reijneveld, Jaap C. Nilsson, R. Jonas A. Tannous, Bakhos A. Wesseling, Pieter Wurdinger, Thomas Cancer Cell Article Tumor-educated blood platelets (TEPs) are implicated as central players in the systemic and local responses to tumor growth, thereby altering their RNA profile. We determined the diagnostic potential of TEPs by mRNA sequencing of 283 platelet samples. We distinguished 228 patients with localized and metastasized tumors from 55 healthy individuals with 96% accuracy. Across six different tumor types, the location of the primary tumor was correctly identified with 71% accuracy. Also, MET or HER2-positive, and mutant KRAS, EGFR, or PIK3CA tumors were accurately distinguished using surrogate TEP mRNA profiles. Our results indicate that blood platelets provide a valuable platform for pan-cancer, multiclass cancer, and companion diagnostics, possibly enabling clinical advances in blood-based “liquid biopsies”. Cell Press 2015-11-09 /pmc/articles/PMC4644263/ /pubmed/26525104 http://dx.doi.org/10.1016/j.ccell.2015.09.018 Text en © 2015 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Best, Myron G.
Sol, Nik
Kooi, Irsan
Tannous, Jihane
Westerman, Bart A.
Rustenburg, François
Schellen, Pepijn
Verschueren, Heleen
Post, Edward
Koster, Jan
Ylstra, Bauke
Ameziane, Najim
Dorsman, Josephine
Smit, Egbert F.
Verheul, Henk M.
Noske, David P.
Reijneveld, Jaap C.
Nilsson, R. Jonas A.
Tannous, Bakhos A.
Wesseling, Pieter
Wurdinger, Thomas
RNA-Seq of Tumor-Educated Platelets Enables Blood-Based Pan-Cancer, Multiclass, and Molecular Pathway Cancer Diagnostics
title RNA-Seq of Tumor-Educated Platelets Enables Blood-Based Pan-Cancer, Multiclass, and Molecular Pathway Cancer Diagnostics
title_full RNA-Seq of Tumor-Educated Platelets Enables Blood-Based Pan-Cancer, Multiclass, and Molecular Pathway Cancer Diagnostics
title_fullStr RNA-Seq of Tumor-Educated Platelets Enables Blood-Based Pan-Cancer, Multiclass, and Molecular Pathway Cancer Diagnostics
title_full_unstemmed RNA-Seq of Tumor-Educated Platelets Enables Blood-Based Pan-Cancer, Multiclass, and Molecular Pathway Cancer Diagnostics
title_short RNA-Seq of Tumor-Educated Platelets Enables Blood-Based Pan-Cancer, Multiclass, and Molecular Pathway Cancer Diagnostics
title_sort rna-seq of tumor-educated platelets enables blood-based pan-cancer, multiclass, and molecular pathway cancer diagnostics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4644263/
https://www.ncbi.nlm.nih.gov/pubmed/26525104
http://dx.doi.org/10.1016/j.ccell.2015.09.018
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