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Vaginal Protection by H(2)O(2)-Producing Lactobacilli

BACKGROUND: Peroxide-producing lactobacilli provide protection from infection for the female reproductive tract. However, in vitro studies demonstrated that H(2)O(2)-produced by Lactobacillus is not the cause of inhibition of pathogens. It is not exactly known how H(2)O(2)-producing lactobacilli are...

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Autores principales: V. Sgibnev, Andrey, A. Kremleva, Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kowsar 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4644264/
https://www.ncbi.nlm.nih.gov/pubmed/26587206
http://dx.doi.org/10.5812/jjm.22913
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author V. Sgibnev, Andrey
A. Kremleva, Elena
author_facet V. Sgibnev, Andrey
A. Kremleva, Elena
author_sort V. Sgibnev, Andrey
collection PubMed
description BACKGROUND: Peroxide-producing lactobacilli provide protection from infection for the female reproductive tract. However, in vitro studies demonstrated that H(2)O(2)-produced by Lactobacillus is not the cause of inhibition of pathogens. It is not exactly known how H(2)O(2)-producing lactobacilli are involved in the protection of the vaginal environment. OBJECTIVES: This study aimed to evaluate the importance of the interaction between H(2)O(2)-producing lactobacilli and their host for the resistance of the vaginal biotope. MATERIALS AND METHODS: In this study, we used vaginal lactobacilli (11 H(2)O(2)-roducing strains and 11 non-H(2)O(2)-producing strains). The influence of epithelial cells on the growth and antibacterial activity of lactobacilli were evaluated. The effects of lactobacilli on the antibacterial activity of the epithelial cells, muramidase and lactoferrin were also determined. RESULTS: Vaginal epithelial cells stimulated the growth and antibacterial activity of H(2)O(2)-producing lactobacilli in a greater extent than that of the non-H(2)O(2)-producing lactobacilli. Mainly, the H(2)O(2)-producing lactobacilli were capable of increasing the activity of the host antimicrobial peptides (muramidase and lactoferrin) as well as the antibacterial activity of the epithelial cells. CONCLUSIONS: The involvement of the peroxide-producing lactobacilli in the protection of vagina was due to their ability to effectively interact with the host. This is expressed on one side to stimulate the growth and antagonistic activity of lactobacilli and on the other side to increase the antibacterial activity of the host defense factors (muramidase, lactoferrin and metabolites of epithelial cells).
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spelling pubmed-46442642015-11-19 Vaginal Protection by H(2)O(2)-Producing Lactobacilli V. Sgibnev, Andrey A. Kremleva, Elena Jundishapur J Microbiol Brief Report BACKGROUND: Peroxide-producing lactobacilli provide protection from infection for the female reproductive tract. However, in vitro studies demonstrated that H(2)O(2)-produced by Lactobacillus is not the cause of inhibition of pathogens. It is not exactly known how H(2)O(2)-producing lactobacilli are involved in the protection of the vaginal environment. OBJECTIVES: This study aimed to evaluate the importance of the interaction between H(2)O(2)-producing lactobacilli and their host for the resistance of the vaginal biotope. MATERIALS AND METHODS: In this study, we used vaginal lactobacilli (11 H(2)O(2)-roducing strains and 11 non-H(2)O(2)-producing strains). The influence of epithelial cells on the growth and antibacterial activity of lactobacilli were evaluated. The effects of lactobacilli on the antibacterial activity of the epithelial cells, muramidase and lactoferrin were also determined. RESULTS: Vaginal epithelial cells stimulated the growth and antibacterial activity of H(2)O(2)-producing lactobacilli in a greater extent than that of the non-H(2)O(2)-producing lactobacilli. Mainly, the H(2)O(2)-producing lactobacilli were capable of increasing the activity of the host antimicrobial peptides (muramidase and lactoferrin) as well as the antibacterial activity of the epithelial cells. CONCLUSIONS: The involvement of the peroxide-producing lactobacilli in the protection of vagina was due to their ability to effectively interact with the host. This is expressed on one side to stimulate the growth and antagonistic activity of lactobacilli and on the other side to increase the antibacterial activity of the host defense factors (muramidase, lactoferrin and metabolites of epithelial cells). Kowsar 2015-10-17 /pmc/articles/PMC4644264/ /pubmed/26587206 http://dx.doi.org/10.5812/jjm.22913 Text en Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
spellingShingle Brief Report
V. Sgibnev, Andrey
A. Kremleva, Elena
Vaginal Protection by H(2)O(2)-Producing Lactobacilli
title Vaginal Protection by H(2)O(2)-Producing Lactobacilli
title_full Vaginal Protection by H(2)O(2)-Producing Lactobacilli
title_fullStr Vaginal Protection by H(2)O(2)-Producing Lactobacilli
title_full_unstemmed Vaginal Protection by H(2)O(2)-Producing Lactobacilli
title_short Vaginal Protection by H(2)O(2)-Producing Lactobacilli
title_sort vaginal protection by h(2)o(2)-producing lactobacilli
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4644264/
https://www.ncbi.nlm.nih.gov/pubmed/26587206
http://dx.doi.org/10.5812/jjm.22913
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