Expression of Melanocortin-4 Receptor mRNA in Male Rat Hypothalamus During Chronic Stress

The effects of chronic stress and glucocorticoids receptor antagonist (RU486) on expression of melanocortin 4 receptor (MC4R) mRNA in arcuate nucleus (ARC) of male rats were evaluated. In this study, adult male Sprague Dawley rats were placed into four groups (n=6/group); stress, RU486, stress/RU486...

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Detalles Bibliográficos
Autores principales: Karami Kheirabad, Maryam, Namavar Jahromi, Bahia, Tamadon, Amin, Ramezani, Amin, Ahmadloo, Somayeh, Sabet Sarvestan, Fatemeh, Koohi-Hosseinabadi, Omid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Babol University of Medical Sciences 2015
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4644530/
https://www.ncbi.nlm.nih.gov/pubmed/26629487
Descripción
Sumario:The effects of chronic stress and glucocorticoids receptor antagonist (RU486) on expression of melanocortin 4 receptor (MC4R) mRNA in arcuate nucleus (ARC) of male rats were evaluated. In this study, adult male Sprague Dawley rats were placed into four groups (n=6/group); stress, RU486, stress/RU486, and control groups. In stress group, the rats were restrained, 1 h/day, for 12 days. In RU486 group, the rats were injected RU486 for 12 days. In stress/RU486 group, the rats were injected RU486 1 h before the stress process for 12 days. Relative expression of MC4R mRNA was determined using real-time PCR. Relative expression of MC4R mRNA in the stress group was higher than that of the control rats (P<0.05). Relative expressions of MC4R mRNA were not different between the stress, RU486 and stress/RU486 groups (P>0.05). Chronic restraint stress causes increase in mRNA expression of MC4R in ARC and blockade of glucocorticoid receptors has no effect on this up-regulation.

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