Expression of Melanocortin-4 Receptor mRNA in Male Rat Hypothalamus During Chronic Stress

The effects of chronic stress and glucocorticoids receptor antagonist (RU486) on expression of melanocortin 4 receptor (MC4R) mRNA in arcuate nucleus (ARC) of male rats were evaluated. In this study, adult male Sprague Dawley rats were placed into four groups (n=6/group); stress, RU486, stress/RU486...

Descripción completa

Detalles Bibliográficos
Autores principales: Karami Kheirabad, Maryam, Namavar Jahromi, Bahia, Tamadon, Amin, Ramezani, Amin, Ahmadloo, Somayeh, Sabet Sarvestan, Fatemeh, Koohi-Hosseinabadi, Omid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Babol University of Medical Sciences 2015
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4644530/
https://www.ncbi.nlm.nih.gov/pubmed/26629487
_version_ 1782400667007057920
author Karami Kheirabad, Maryam
Namavar Jahromi, Bahia
Tamadon, Amin
Ramezani, Amin
Ahmadloo, Somayeh
Sabet Sarvestan, Fatemeh
Koohi-Hosseinabadi, Omid
author_facet Karami Kheirabad, Maryam
Namavar Jahromi, Bahia
Tamadon, Amin
Ramezani, Amin
Ahmadloo, Somayeh
Sabet Sarvestan, Fatemeh
Koohi-Hosseinabadi, Omid
author_sort Karami Kheirabad, Maryam
collection PubMed
description The effects of chronic stress and glucocorticoids receptor antagonist (RU486) on expression of melanocortin 4 receptor (MC4R) mRNA in arcuate nucleus (ARC) of male rats were evaluated. In this study, adult male Sprague Dawley rats were placed into four groups (n=6/group); stress, RU486, stress/RU486, and control groups. In stress group, the rats were restrained, 1 h/day, for 12 days. In RU486 group, the rats were injected RU486 for 12 days. In stress/RU486 group, the rats were injected RU486 1 h before the stress process for 12 days. Relative expression of MC4R mRNA was determined using real-time PCR. Relative expression of MC4R mRNA in the stress group was higher than that of the control rats (P<0.05). Relative expressions of MC4R mRNA were not different between the stress, RU486 and stress/RU486 groups (P>0.05). Chronic restraint stress causes increase in mRNA expression of MC4R in ARC and blockade of glucocorticoid receptors has no effect on this up-regulation.
format Online
Article
Text
id pubmed-4644530
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Babol University of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-46445302015-12-01 Expression of Melanocortin-4 Receptor mRNA in Male Rat Hypothalamus During Chronic Stress Karami Kheirabad, Maryam Namavar Jahromi, Bahia Tamadon, Amin Ramezani, Amin Ahmadloo, Somayeh Sabet Sarvestan, Fatemeh Koohi-Hosseinabadi, Omid Int J Mol Cell Med Original Article The effects of chronic stress and glucocorticoids receptor antagonist (RU486) on expression of melanocortin 4 receptor (MC4R) mRNA in arcuate nucleus (ARC) of male rats were evaluated. In this study, adult male Sprague Dawley rats were placed into four groups (n=6/group); stress, RU486, stress/RU486, and control groups. In stress group, the rats were restrained, 1 h/day, for 12 days. In RU486 group, the rats were injected RU486 for 12 days. In stress/RU486 group, the rats were injected RU486 1 h before the stress process for 12 days. Relative expression of MC4R mRNA was determined using real-time PCR. Relative expression of MC4R mRNA in the stress group was higher than that of the control rats (P<0.05). Relative expressions of MC4R mRNA were not different between the stress, RU486 and stress/RU486 groups (P>0.05). Chronic restraint stress causes increase in mRNA expression of MC4R in ARC and blockade of glucocorticoid receptors has no effect on this up-regulation. Babol University of Medical Sciences 2015 /pmc/articles/PMC4644530/ /pubmed/26629487 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Karami Kheirabad, Maryam
Namavar Jahromi, Bahia
Tamadon, Amin
Ramezani, Amin
Ahmadloo, Somayeh
Sabet Sarvestan, Fatemeh
Koohi-Hosseinabadi, Omid
Expression of Melanocortin-4 Receptor mRNA in Male Rat Hypothalamus During Chronic Stress
title Expression of Melanocortin-4 Receptor mRNA in Male Rat Hypothalamus During Chronic Stress
title_full Expression of Melanocortin-4 Receptor mRNA in Male Rat Hypothalamus During Chronic Stress
title_fullStr Expression of Melanocortin-4 Receptor mRNA in Male Rat Hypothalamus During Chronic Stress
title_full_unstemmed Expression of Melanocortin-4 Receptor mRNA in Male Rat Hypothalamus During Chronic Stress
title_short Expression of Melanocortin-4 Receptor mRNA in Male Rat Hypothalamus During Chronic Stress
title_sort expression of melanocortin-4 receptor mrna in male rat hypothalamus during chronic stress
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4644530/
https://www.ncbi.nlm.nih.gov/pubmed/26629487
work_keys_str_mv AT karamikheirabadmaryam expressionofmelanocortin4receptormrnainmalerathypothalamusduringchronicstress
AT namavarjahromibahia expressionofmelanocortin4receptormrnainmalerathypothalamusduringchronicstress
AT tamadonamin expressionofmelanocortin4receptormrnainmalerathypothalamusduringchronicstress
AT ramezaniamin expressionofmelanocortin4receptormrnainmalerathypothalamusduringchronicstress
AT ahmadloosomayeh expressionofmelanocortin4receptormrnainmalerathypothalamusduringchronicstress
AT sabetsarvestanfatemeh expressionofmelanocortin4receptormrnainmalerathypothalamusduringchronicstress
AT koohihosseinabadiomid expressionofmelanocortin4receptormrnainmalerathypothalamusduringchronicstress

Ejemplares similares