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Genomic Evidence Reveals Numerous Salmonella enterica Serovar Newport Reintroduction Events in Suwannee Watershed Irrigation Ponds

Our previous work indicated a predominance (56.8%) of Salmonella enterica serovar Newport among isolates recovered from irrigation ponds used in produce farms over a 2-year period (B. Li et al., Appl Environ Microbiol 80:6355–6365, http://dx.doi.org/10.1128/AEM.02063-14). This observation provided a...

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Autores principales: Li, Baoguang, Jackson, Scott A., Gangiredla, Jayanthi, Wang, Weimin, Liu, Huanli, Tall, Ben D., Beaubrun, Junia Jean-Gilles, Jay-Russell, Michele, Vellidis, George, Elkins, Christopher A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4644655/
https://www.ncbi.nlm.nih.gov/pubmed/26386063
http://dx.doi.org/10.1128/AEM.02179-15
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author Li, Baoguang
Jackson, Scott A.
Gangiredla, Jayanthi
Wang, Weimin
Liu, Huanli
Tall, Ben D.
Beaubrun, Junia Jean-Gilles
Jay-Russell, Michele
Vellidis, George
Elkins, Christopher A.
author_facet Li, Baoguang
Jackson, Scott A.
Gangiredla, Jayanthi
Wang, Weimin
Liu, Huanli
Tall, Ben D.
Beaubrun, Junia Jean-Gilles
Jay-Russell, Michele
Vellidis, George
Elkins, Christopher A.
author_sort Li, Baoguang
collection PubMed
description Our previous work indicated a predominance (56.8%) of Salmonella enterica serovar Newport among isolates recovered from irrigation ponds used in produce farms over a 2-year period (B. Li et al., Appl Environ Microbiol 80:6355–6365, http://dx.doi.org/10.1128/AEM.02063-14). This observation provided a valuable set of metrics to explore an underaddressed issue of environmental survival of Salmonella by DNA microarray. Microarray analysis correctly identified all the isolates (n = 53) and differentiated the S. Newport isolates into two phylogenetic lineages (S. Newport II and S. Newport III). Serovar distribution analysis showed no instances where the same serovar was recovered from a pond for more than a month. Furthermore, during the study, numerous isolates with an indistinguishable genotype were recovered from different ponds as far as 180 km apart for time intervals as long as 2 years. Although isolates within either lineage were phylogenetically related as determined by microarray analysis, subtle genotypic differences were detected within the lineages, suggesting that isolates in either lineage could have come from several unique hosts. For example, strains in four different subgroups (A, B, C, and D) possessed an indistinguishable genotype within their subgroups as measured by gene differences, suggesting that strains in each subgroup shared a common host. Based on this comparative genomic evidence and the spatial and temporal factors, we speculated that the presence of Salmonella in the ponds was likely due to numerous punctuated reintroduction events associated with several different but common hosts in the environment. These findings may have implications for the development of strategies for efficient and safe irrigation to minimize the risk of Salmonella outbreaks associated with fresh produce.
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spelling pubmed-46446552015-11-20 Genomic Evidence Reveals Numerous Salmonella enterica Serovar Newport Reintroduction Events in Suwannee Watershed Irrigation Ponds Li, Baoguang Jackson, Scott A. Gangiredla, Jayanthi Wang, Weimin Liu, Huanli Tall, Ben D. Beaubrun, Junia Jean-Gilles Jay-Russell, Michele Vellidis, George Elkins, Christopher A. Appl Environ Microbiol Public and Environmental Health Microbiology Our previous work indicated a predominance (56.8%) of Salmonella enterica serovar Newport among isolates recovered from irrigation ponds used in produce farms over a 2-year period (B. Li et al., Appl Environ Microbiol 80:6355–6365, http://dx.doi.org/10.1128/AEM.02063-14). This observation provided a valuable set of metrics to explore an underaddressed issue of environmental survival of Salmonella by DNA microarray. Microarray analysis correctly identified all the isolates (n = 53) and differentiated the S. Newport isolates into two phylogenetic lineages (S. Newport II and S. Newport III). Serovar distribution analysis showed no instances where the same serovar was recovered from a pond for more than a month. Furthermore, during the study, numerous isolates with an indistinguishable genotype were recovered from different ponds as far as 180 km apart for time intervals as long as 2 years. Although isolates within either lineage were phylogenetically related as determined by microarray analysis, subtle genotypic differences were detected within the lineages, suggesting that isolates in either lineage could have come from several unique hosts. For example, strains in four different subgroups (A, B, C, and D) possessed an indistinguishable genotype within their subgroups as measured by gene differences, suggesting that strains in each subgroup shared a common host. Based on this comparative genomic evidence and the spatial and temporal factors, we speculated that the presence of Salmonella in the ponds was likely due to numerous punctuated reintroduction events associated with several different but common hosts in the environment. These findings may have implications for the development of strategies for efficient and safe irrigation to minimize the risk of Salmonella outbreaks associated with fresh produce. American Society for Microbiology 2015-11-13 2015-12 /pmc/articles/PMC4644655/ /pubmed/26386063 http://dx.doi.org/10.1128/AEM.02179-15 Text en Copyright © 2015 Li et al. http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license (http://creativecommons.org/licenses/by-nc-sa/3.0/) , which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Public and Environmental Health Microbiology
Li, Baoguang
Jackson, Scott A.
Gangiredla, Jayanthi
Wang, Weimin
Liu, Huanli
Tall, Ben D.
Beaubrun, Junia Jean-Gilles
Jay-Russell, Michele
Vellidis, George
Elkins, Christopher A.
Genomic Evidence Reveals Numerous Salmonella enterica Serovar Newport Reintroduction Events in Suwannee Watershed Irrigation Ponds
title Genomic Evidence Reveals Numerous Salmonella enterica Serovar Newport Reintroduction Events in Suwannee Watershed Irrigation Ponds
title_full Genomic Evidence Reveals Numerous Salmonella enterica Serovar Newport Reintroduction Events in Suwannee Watershed Irrigation Ponds
title_fullStr Genomic Evidence Reveals Numerous Salmonella enterica Serovar Newport Reintroduction Events in Suwannee Watershed Irrigation Ponds
title_full_unstemmed Genomic Evidence Reveals Numerous Salmonella enterica Serovar Newport Reintroduction Events in Suwannee Watershed Irrigation Ponds
title_short Genomic Evidence Reveals Numerous Salmonella enterica Serovar Newport Reintroduction Events in Suwannee Watershed Irrigation Ponds
title_sort genomic evidence reveals numerous salmonella enterica serovar newport reintroduction events in suwannee watershed irrigation ponds
topic Public and Environmental Health Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4644655/
https://www.ncbi.nlm.nih.gov/pubmed/26386063
http://dx.doi.org/10.1128/AEM.02179-15
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