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Organ-wide 3D-imaging and topological analysis of the continuous microvascular network in a murine lymph node

Understanding of the microvasculature has previously been limited by the lack of methods capable of capturing and modelling complete vascular networks. We used novel imaging and computational techniques to establish the topology of the entire blood vessel network of a murine lymph node, combining 63...

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Autores principales: Kelch, Inken D., Bogle, Gib, Sands, Gregory B., Phillips, Anthony R. J., LeGrice, Ian J., Rod Dunbar, P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4645097/
https://www.ncbi.nlm.nih.gov/pubmed/26567707
http://dx.doi.org/10.1038/srep16534
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author Kelch, Inken D.
Bogle, Gib
Sands, Gregory B.
Phillips, Anthony R. J.
LeGrice, Ian J.
Rod Dunbar, P.
author_facet Kelch, Inken D.
Bogle, Gib
Sands, Gregory B.
Phillips, Anthony R. J.
LeGrice, Ian J.
Rod Dunbar, P.
author_sort Kelch, Inken D.
collection PubMed
description Understanding of the microvasculature has previously been limited by the lack of methods capable of capturing and modelling complete vascular networks. We used novel imaging and computational techniques to establish the topology of the entire blood vessel network of a murine lymph node, combining 63706 confocal images at 2 μm pixel resolution to cover a volume of 3.88 mm(3). Detailed measurements including the distribution of vessel diameters, branch counts, and identification of voids were subsequently re-visualised in 3D revealing regional specialisation within the network. By focussing on critical immune microenvironments we quantified differences in their vascular topology. We further developed a morphology-based approach to identify High Endothelial Venules, key sites for lymphocyte extravasation. These data represent a comprehensive and continuous blood vessel network of an entire organ and provide benchmark measurements that will inform modelling of blood vessel networks as well as enable comparison of vascular topology in different organs.
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spelling pubmed-46450972015-11-20 Organ-wide 3D-imaging and topological analysis of the continuous microvascular network in a murine lymph node Kelch, Inken D. Bogle, Gib Sands, Gregory B. Phillips, Anthony R. J. LeGrice, Ian J. Rod Dunbar, P. Sci Rep Article Understanding of the microvasculature has previously been limited by the lack of methods capable of capturing and modelling complete vascular networks. We used novel imaging and computational techniques to establish the topology of the entire blood vessel network of a murine lymph node, combining 63706 confocal images at 2 μm pixel resolution to cover a volume of 3.88 mm(3). Detailed measurements including the distribution of vessel diameters, branch counts, and identification of voids were subsequently re-visualised in 3D revealing regional specialisation within the network. By focussing on critical immune microenvironments we quantified differences in their vascular topology. We further developed a morphology-based approach to identify High Endothelial Venules, key sites for lymphocyte extravasation. These data represent a comprehensive and continuous blood vessel network of an entire organ and provide benchmark measurements that will inform modelling of blood vessel networks as well as enable comparison of vascular topology in different organs. Nature Publishing Group 2015-11-16 /pmc/articles/PMC4645097/ /pubmed/26567707 http://dx.doi.org/10.1038/srep16534 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Kelch, Inken D.
Bogle, Gib
Sands, Gregory B.
Phillips, Anthony R. J.
LeGrice, Ian J.
Rod Dunbar, P.
Organ-wide 3D-imaging and topological analysis of the continuous microvascular network in a murine lymph node
title Organ-wide 3D-imaging and topological analysis of the continuous microvascular network in a murine lymph node
title_full Organ-wide 3D-imaging and topological analysis of the continuous microvascular network in a murine lymph node
title_fullStr Organ-wide 3D-imaging and topological analysis of the continuous microvascular network in a murine lymph node
title_full_unstemmed Organ-wide 3D-imaging and topological analysis of the continuous microvascular network in a murine lymph node
title_short Organ-wide 3D-imaging and topological analysis of the continuous microvascular network in a murine lymph node
title_sort organ-wide 3d-imaging and topological analysis of the continuous microvascular network in a murine lymph node
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4645097/
https://www.ncbi.nlm.nih.gov/pubmed/26567707
http://dx.doi.org/10.1038/srep16534
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