Epstein-Barr Viruses (EBVs) Deficient in EBV-Encoded RNAs Have Higher Levels of Latent Membrane Protein 2 RNA Expression in Lymphoblastoid Cell Lines and Efficiently Establish Persistent Infections in Humanized Mice

Functions of Epstein-Barr virus (EBV)-encoded RNAs (EBERs) were tested in lymphoblastoid cell lines containing EBER mutants of EBV. Binding of EBER1 to ribosomal protein L22 (RPL22) was confirmed. Deletion of EBER1 or EBER2 correlated with increased levels of cytoplasmic EBV LMP2 RNA and with small...

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Detalles Bibliográficos
Autores principales: Gregorovic, Goran, Boulden, Elizabeth A., Bosshard, Rachel, Elgueta Karstegl, Claudio, Skalsky, Rebecca, Cullen, Bryan R., Gujer, Cornelia, Rämer, Patrick, Münz, Christian, Farrell, Paul J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2015
Materias:
Virus-Cell Interactions
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4645642/
https://www.ncbi.nlm.nih.gov/pubmed/26339045
http://dx.doi.org/10.1128/JVI.01873-15
Descripción
Sumario:Functions of Epstein-Barr virus (EBV)-encoded RNAs (EBERs) were tested in lymphoblastoid cell lines containing EBER mutants of EBV. Binding of EBER1 to ribosomal protein L22 (RPL22) was confirmed. Deletion of EBER1 or EBER2 correlated with increased levels of cytoplasmic EBV LMP2 RNA and with small effects on specific cellular microRNA (miRNA) levels, but protein levels of LMP1 and LMP2A were not affected. Wild-type EBV and EBER deletion EBV had approximately equal abilities to infect immunodeficient mice reconstituted with a human hematopoietic system.

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