Epstein-Barr Viruses (EBVs) Deficient in EBV-Encoded RNAs Have Higher Levels of Latent Membrane Protein 2 RNA Expression in Lymphoblastoid Cell Lines and Efficiently Establish Persistent Infections in Humanized Mice

Functions of Epstein-Barr virus (EBV)-encoded RNAs (EBERs) were tested in lymphoblastoid cell lines containing EBER mutants of EBV. Binding of EBER1 to ribosomal protein L22 (RPL22) was confirmed. Deletion of EBER1 or EBER2 correlated with increased levels of cytoplasmic EBV LMP2 RNA and with small...

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Autores principales: Gregorovic, Goran, Boulden, Elizabeth A., Bosshard, Rachel, Elgueta Karstegl, Claudio, Skalsky, Rebecca, Cullen, Bryan R., Gujer, Cornelia, Rämer, Patrick, Münz, Christian, Farrell, Paul J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2015
Materias:
Virus-Cell Interactions
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4645642/
https://www.ncbi.nlm.nih.gov/pubmed/26339045
http://dx.doi.org/10.1128/JVI.01873-15
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author Gregorovic, Goran
Boulden, Elizabeth A.
Bosshard, Rachel
Elgueta Karstegl, Claudio
Skalsky, Rebecca
Cullen, Bryan R.
Gujer, Cornelia
Rämer, Patrick
Münz, Christian
Farrell, Paul J.
author_facet Gregorovic, Goran
Boulden, Elizabeth A.
Bosshard, Rachel
Elgueta Karstegl, Claudio
Skalsky, Rebecca
Cullen, Bryan R.
Gujer, Cornelia
Rämer, Patrick
Münz, Christian
Farrell, Paul J.
author_sort Gregorovic, Goran
collection PubMed
description Functions of Epstein-Barr virus (EBV)-encoded RNAs (EBERs) were tested in lymphoblastoid cell lines containing EBER mutants of EBV. Binding of EBER1 to ribosomal protein L22 (RPL22) was confirmed. Deletion of EBER1 or EBER2 correlated with increased levels of cytoplasmic EBV LMP2 RNA and with small effects on specific cellular microRNA (miRNA) levels, but protein levels of LMP1 and LMP2A were not affected. Wild-type EBV and EBER deletion EBV had approximately equal abilities to infect immunodeficient mice reconstituted with a human hematopoietic system.
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spelling pubmed-46456422015-12-10 Epstein-Barr Viruses (EBVs) Deficient in EBV-Encoded RNAs Have Higher Levels of Latent Membrane Protein 2 RNA Expression in Lymphoblastoid Cell Lines and Efficiently Establish Persistent Infections in Humanized Mice Gregorovic, Goran Boulden, Elizabeth A. Bosshard, Rachel Elgueta Karstegl, Claudio Skalsky, Rebecca Cullen, Bryan R. Gujer, Cornelia Rämer, Patrick Münz, Christian Farrell, Paul J. J Virol Virus-Cell Interactions Functions of Epstein-Barr virus (EBV)-encoded RNAs (EBERs) were tested in lymphoblastoid cell lines containing EBER mutants of EBV. Binding of EBER1 to ribosomal protein L22 (RPL22) was confirmed. Deletion of EBER1 or EBER2 correlated with increased levels of cytoplasmic EBV LMP2 RNA and with small effects on specific cellular microRNA (miRNA) levels, but protein levels of LMP1 and LMP2A were not affected. Wild-type EBV and EBER deletion EBV had approximately equal abilities to infect immunodeficient mice reconstituted with a human hematopoietic system. American Society for Microbiology 2015-09-02 /pmc/articles/PMC4645642/ /pubmed/26339045 http://dx.doi.org/10.1128/JVI.01873-15 Text en Copyright © 2015, Gregorovic et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Virus-Cell Interactions
Gregorovic, Goran
Boulden, Elizabeth A.
Bosshard, Rachel
Elgueta Karstegl, Claudio
Skalsky, Rebecca
Cullen, Bryan R.
Gujer, Cornelia
Rämer, Patrick
Münz, Christian
Farrell, Paul J.
Epstein-Barr Viruses (EBVs) Deficient in EBV-Encoded RNAs Have Higher Levels of Latent Membrane Protein 2 RNA Expression in Lymphoblastoid Cell Lines and Efficiently Establish Persistent Infections in Humanized Mice
title Epstein-Barr Viruses (EBVs) Deficient in EBV-Encoded RNAs Have Higher Levels of Latent Membrane Protein 2 RNA Expression in Lymphoblastoid Cell Lines and Efficiently Establish Persistent Infections in Humanized Mice
title_full Epstein-Barr Viruses (EBVs) Deficient in EBV-Encoded RNAs Have Higher Levels of Latent Membrane Protein 2 RNA Expression in Lymphoblastoid Cell Lines and Efficiently Establish Persistent Infections in Humanized Mice
title_fullStr Epstein-Barr Viruses (EBVs) Deficient in EBV-Encoded RNAs Have Higher Levels of Latent Membrane Protein 2 RNA Expression in Lymphoblastoid Cell Lines and Efficiently Establish Persistent Infections in Humanized Mice
title_full_unstemmed Epstein-Barr Viruses (EBVs) Deficient in EBV-Encoded RNAs Have Higher Levels of Latent Membrane Protein 2 RNA Expression in Lymphoblastoid Cell Lines and Efficiently Establish Persistent Infections in Humanized Mice
title_short Epstein-Barr Viruses (EBVs) Deficient in EBV-Encoded RNAs Have Higher Levels of Latent Membrane Protein 2 RNA Expression in Lymphoblastoid Cell Lines and Efficiently Establish Persistent Infections in Humanized Mice
title_sort epstein-barr viruses (ebvs) deficient in ebv-encoded rnas have higher levels of latent membrane protein 2 rna expression in lymphoblastoid cell lines and efficiently establish persistent infections in humanized mice
topic Virus-Cell Interactions
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4645642/
https://www.ncbi.nlm.nih.gov/pubmed/26339045
http://dx.doi.org/10.1128/JVI.01873-15
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