Exclusive Decoration of Simian Immunodeficiency Virus Env with High-Mannose Type N-Glycans Is Not Compatible with Mucosal Transmission in Rhesus Macaques

Human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) envelope (Env) proteins are extensively decorated with N-glycans, predominantly of the high-mannose type. However, it is unclear how high-mannose N-glycans on Env impact viral spread. We show that exclusive modification of SI...

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Autores principales: Karsten, Christina B., Buettner, Falk F. R., Cajic, Samanta, Nehlmeier, Inga, Neumann, Berit, Klippert, Antonina, Sauermann, Ulrike, Reichl, Udo, Gerardy-Schahn, Rita, Rapp, Erdmann, Stahl-Hennig, Christiane, Pöhlmann, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2015
Materias:
Virus-Cell Interactions
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4645679/
https://www.ncbi.nlm.nih.gov/pubmed/26355090
http://dx.doi.org/10.1128/JVI.01358-15
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author Karsten, Christina B.
Buettner, Falk F. R.
Cajic, Samanta
Nehlmeier, Inga
Neumann, Berit
Klippert, Antonina
Sauermann, Ulrike
Reichl, Udo
Gerardy-Schahn, Rita
Rapp, Erdmann
Stahl-Hennig, Christiane
Pöhlmann, Stefan
author_facet Karsten, Christina B.
Buettner, Falk F. R.
Cajic, Samanta
Nehlmeier, Inga
Neumann, Berit
Klippert, Antonina
Sauermann, Ulrike
Reichl, Udo
Gerardy-Schahn, Rita
Rapp, Erdmann
Stahl-Hennig, Christiane
Pöhlmann, Stefan
author_sort Karsten, Christina B.
collection PubMed
description Human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) envelope (Env) proteins are extensively decorated with N-glycans, predominantly of the high-mannose type. However, it is unclear how high-mannose N-glycans on Env impact viral spread. We show that exclusive modification of SIV Env with these N-glycans reduces viral infectivity and abrogates mucosal transmission, despite increasing viral capture by immune cell lectins. Thus, high-mannose N-glycans have opposed effects on SIV infectivity and lectin reactivity, and a balance might be required for efficient mucosal transmission.
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spelling pubmed-46456792015-12-10 Exclusive Decoration of Simian Immunodeficiency Virus Env with High-Mannose Type N-Glycans Is Not Compatible with Mucosal Transmission in Rhesus Macaques Karsten, Christina B. Buettner, Falk F. R. Cajic, Samanta Nehlmeier, Inga Neumann, Berit Klippert, Antonina Sauermann, Ulrike Reichl, Udo Gerardy-Schahn, Rita Rapp, Erdmann Stahl-Hennig, Christiane Pöhlmann, Stefan J Virol Virus-Cell Interactions Human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) envelope (Env) proteins are extensively decorated with N-glycans, predominantly of the high-mannose type. However, it is unclear how high-mannose N-glycans on Env impact viral spread. We show that exclusive modification of SIV Env with these N-glycans reduces viral infectivity and abrogates mucosal transmission, despite increasing viral capture by immune cell lectins. Thus, high-mannose N-glycans have opposed effects on SIV infectivity and lectin reactivity, and a balance might be required for efficient mucosal transmission. American Society for Microbiology 2015-09-09 /pmc/articles/PMC4645679/ /pubmed/26355090 http://dx.doi.org/10.1128/JVI.01358-15 Text en Copyright © 2015, Karsten et al. http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license (http://creativecommons.org/licenses/by-nc-sa/3.0/) , which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Virus-Cell Interactions
Karsten, Christina B.
Buettner, Falk F. R.
Cajic, Samanta
Nehlmeier, Inga
Neumann, Berit
Klippert, Antonina
Sauermann, Ulrike
Reichl, Udo
Gerardy-Schahn, Rita
Rapp, Erdmann
Stahl-Hennig, Christiane
Pöhlmann, Stefan
Exclusive Decoration of Simian Immunodeficiency Virus Env with High-Mannose Type N-Glycans Is Not Compatible with Mucosal Transmission in Rhesus Macaques
title Exclusive Decoration of Simian Immunodeficiency Virus Env with High-Mannose Type N-Glycans Is Not Compatible with Mucosal Transmission in Rhesus Macaques
title_full Exclusive Decoration of Simian Immunodeficiency Virus Env with High-Mannose Type N-Glycans Is Not Compatible with Mucosal Transmission in Rhesus Macaques
title_fullStr Exclusive Decoration of Simian Immunodeficiency Virus Env with High-Mannose Type N-Glycans Is Not Compatible with Mucosal Transmission in Rhesus Macaques
title_full_unstemmed Exclusive Decoration of Simian Immunodeficiency Virus Env with High-Mannose Type N-Glycans Is Not Compatible with Mucosal Transmission in Rhesus Macaques
title_short Exclusive Decoration of Simian Immunodeficiency Virus Env with High-Mannose Type N-Glycans Is Not Compatible with Mucosal Transmission in Rhesus Macaques
title_sort exclusive decoration of simian immunodeficiency virus env with high-mannose type n-glycans is not compatible with mucosal transmission in rhesus macaques
topic Virus-Cell Interactions
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4645679/
https://www.ncbi.nlm.nih.gov/pubmed/26355090
http://dx.doi.org/10.1128/JVI.01358-15
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