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Detection of Fungal Elements in Atherosclerotic Plaques Using Mycological, Pathological and Molecular Methods
BACKGROUND: The aim of this study was to detect fungi in atherosclerotic plaques and investigate their possible role in atherosclerosis. METHODS: Coronary atherosclerotic plaques specimen were obtained from patients with atherosclerosis. Direct examination, culture, histopathology study, PCR and seq...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tehran University of Medical Sciences
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4645732/ https://www.ncbi.nlm.nih.gov/pubmed/26587476 |
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author | MASOUMI, Omid SHAHZADI, Mahmoud KORDBACHEH, Parivash ZAINI, Farideh MAHMOUDI, Shahram MAHMOUDI, Mahmoud BAHREINI, Hesamoddin SAFARA, Mahin MIRHENDI, Hossein |
author_facet | MASOUMI, Omid SHAHZADI, Mahmoud KORDBACHEH, Parivash ZAINI, Farideh MAHMOUDI, Shahram MAHMOUDI, Mahmoud BAHREINI, Hesamoddin SAFARA, Mahin MIRHENDI, Hossein |
author_sort | MASOUMI, Omid |
collection | PubMed |
description | BACKGROUND: The aim of this study was to detect fungi in atherosclerotic plaques and investigate their possible role in atherosclerosis. METHODS: Coronary atherosclerotic plaques specimen were obtained from patients with atherosclerosis. Direct examination, culture, histopathology study, PCR and sequencing were performed to detect/identify the mycotic elements in the plaques. Age, sex, smoking, obesity, hypertension, hyperlipidemia, family history of heart diseases and diabetes were considered and data were analyzed using Chi Square test by SPSS version 15. RESULTS: A total of 41 specimens were analyzed. Direct examination for fungal elements was negative in all cases but in culture only one specimen grew as a mold colony. The presence of fungal elements were confirmed in 6 and 2 tissue sections stained by Gomori methenamine silver and Hematoxylin and Eosin methods, respectively. Using PCR, 11 cases were positive for fungi. The DNA sequence analysis of six positive specimens which were randomly selected revealed fungi as Candida albicans (n=3), Candida guilliermondii (n=2) and Monilia sp. (n=1). CONCLUSION: A significant association between the presence of fungi in atherosclerotic plaques and severity of atherogenesis and atherosclerotic disease was not found. This could be due to limited numbers of patients included in our study. However, the presence of fungal elements in 26.8% of our specimens is considerable and the results does not exclude the correlation between the presence of fungi with atherosclerosis and coronary artery disease. |
format | Online Article Text |
id | pubmed-4645732 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Tehran University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-46457322015-11-19 Detection of Fungal Elements in Atherosclerotic Plaques Using Mycological, Pathological and Molecular Methods MASOUMI, Omid SHAHZADI, Mahmoud KORDBACHEH, Parivash ZAINI, Farideh MAHMOUDI, Shahram MAHMOUDI, Mahmoud BAHREINI, Hesamoddin SAFARA, Mahin MIRHENDI, Hossein Iran J Public Health Original Article BACKGROUND: The aim of this study was to detect fungi in atherosclerotic plaques and investigate their possible role in atherosclerosis. METHODS: Coronary atherosclerotic plaques specimen were obtained from patients with atherosclerosis. Direct examination, culture, histopathology study, PCR and sequencing were performed to detect/identify the mycotic elements in the plaques. Age, sex, smoking, obesity, hypertension, hyperlipidemia, family history of heart diseases and diabetes were considered and data were analyzed using Chi Square test by SPSS version 15. RESULTS: A total of 41 specimens were analyzed. Direct examination for fungal elements was negative in all cases but in culture only one specimen grew as a mold colony. The presence of fungal elements were confirmed in 6 and 2 tissue sections stained by Gomori methenamine silver and Hematoxylin and Eosin methods, respectively. Using PCR, 11 cases were positive for fungi. The DNA sequence analysis of six positive specimens which were randomly selected revealed fungi as Candida albicans (n=3), Candida guilliermondii (n=2) and Monilia sp. (n=1). CONCLUSION: A significant association between the presence of fungi in atherosclerotic plaques and severity of atherogenesis and atherosclerotic disease was not found. This could be due to limited numbers of patients included in our study. However, the presence of fungal elements in 26.8% of our specimens is considerable and the results does not exclude the correlation between the presence of fungi with atherosclerosis and coronary artery disease. Tehran University of Medical Sciences 2015-08 /pmc/articles/PMC4645732/ /pubmed/26587476 Text en Copyright© Iranian Public Health Association & Tehran University of Medical Sciences This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly. |
spellingShingle | Original Article MASOUMI, Omid SHAHZADI, Mahmoud KORDBACHEH, Parivash ZAINI, Farideh MAHMOUDI, Shahram MAHMOUDI, Mahmoud BAHREINI, Hesamoddin SAFARA, Mahin MIRHENDI, Hossein Detection of Fungal Elements in Atherosclerotic Plaques Using Mycological, Pathological and Molecular Methods |
title | Detection of Fungal Elements in Atherosclerotic Plaques Using Mycological, Pathological and Molecular Methods |
title_full | Detection of Fungal Elements in Atherosclerotic Plaques Using Mycological, Pathological and Molecular Methods |
title_fullStr | Detection of Fungal Elements in Atherosclerotic Plaques Using Mycological, Pathological and Molecular Methods |
title_full_unstemmed | Detection of Fungal Elements in Atherosclerotic Plaques Using Mycological, Pathological and Molecular Methods |
title_short | Detection of Fungal Elements in Atherosclerotic Plaques Using Mycological, Pathological and Molecular Methods |
title_sort | detection of fungal elements in atherosclerotic plaques using mycological, pathological and molecular methods |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4645732/ https://www.ncbi.nlm.nih.gov/pubmed/26587476 |
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