Cargando…

Detection of Fungal Elements in Atherosclerotic Plaques Using Mycological, Pathological and Molecular Methods

BACKGROUND: The aim of this study was to detect fungi in atherosclerotic plaques and investigate their possible role in atherosclerosis. METHODS: Coronary atherosclerotic plaques specimen were obtained from patients with atherosclerosis. Direct examination, culture, histopathology study, PCR and seq...

Descripción completa

Detalles Bibliográficos
Autores principales: MASOUMI, Omid, SHAHZADI, Mahmoud, KORDBACHEH, Parivash, ZAINI, Farideh, MAHMOUDI, Shahram, MAHMOUDI, Mahmoud, BAHREINI, Hesamoddin, SAFARA, Mahin, MIRHENDI, Hossein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4645732/
https://www.ncbi.nlm.nih.gov/pubmed/26587476
_version_ 1782400852644855808
author MASOUMI, Omid
SHAHZADI, Mahmoud
KORDBACHEH, Parivash
ZAINI, Farideh
MAHMOUDI, Shahram
MAHMOUDI, Mahmoud
BAHREINI, Hesamoddin
SAFARA, Mahin
MIRHENDI, Hossein
author_facet MASOUMI, Omid
SHAHZADI, Mahmoud
KORDBACHEH, Parivash
ZAINI, Farideh
MAHMOUDI, Shahram
MAHMOUDI, Mahmoud
BAHREINI, Hesamoddin
SAFARA, Mahin
MIRHENDI, Hossein
author_sort MASOUMI, Omid
collection PubMed
description BACKGROUND: The aim of this study was to detect fungi in atherosclerotic plaques and investigate their possible role in atherosclerosis. METHODS: Coronary atherosclerotic plaques specimen were obtained from patients with atherosclerosis. Direct examination, culture, histopathology study, PCR and sequencing were performed to detect/identify the mycotic elements in the plaques. Age, sex, smoking, obesity, hypertension, hyperlipidemia, family history of heart diseases and diabetes were considered and data were analyzed using Chi Square test by SPSS version 15. RESULTS: A total of 41 specimens were analyzed. Direct examination for fungal elements was negative in all cases but in culture only one specimen grew as a mold colony. The presence of fungal elements were confirmed in 6 and 2 tissue sections stained by Gomori methenamine silver and Hematoxylin and Eosin methods, respectively. Using PCR, 11 cases were positive for fungi. The DNA sequence analysis of six positive specimens which were randomly selected revealed fungi as Candida albicans (n=3), Candida guilliermondii (n=2) and Monilia sp. (n=1). CONCLUSION: A significant association between the presence of fungi in atherosclerotic plaques and severity of atherogenesis and atherosclerotic disease was not found. This could be due to limited numbers of patients included in our study. However, the presence of fungal elements in 26.8% of our specimens is considerable and the results does not exclude the correlation between the presence of fungi with atherosclerosis and coronary artery disease.
format Online
Article
Text
id pubmed-4645732
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Tehran University of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-46457322015-11-19 Detection of Fungal Elements in Atherosclerotic Plaques Using Mycological, Pathological and Molecular Methods MASOUMI, Omid SHAHZADI, Mahmoud KORDBACHEH, Parivash ZAINI, Farideh MAHMOUDI, Shahram MAHMOUDI, Mahmoud BAHREINI, Hesamoddin SAFARA, Mahin MIRHENDI, Hossein Iran J Public Health Original Article BACKGROUND: The aim of this study was to detect fungi in atherosclerotic plaques and investigate their possible role in atherosclerosis. METHODS: Coronary atherosclerotic plaques specimen were obtained from patients with atherosclerosis. Direct examination, culture, histopathology study, PCR and sequencing were performed to detect/identify the mycotic elements in the plaques. Age, sex, smoking, obesity, hypertension, hyperlipidemia, family history of heart diseases and diabetes were considered and data were analyzed using Chi Square test by SPSS version 15. RESULTS: A total of 41 specimens were analyzed. Direct examination for fungal elements was negative in all cases but in culture only one specimen grew as a mold colony. The presence of fungal elements were confirmed in 6 and 2 tissue sections stained by Gomori methenamine silver and Hematoxylin and Eosin methods, respectively. Using PCR, 11 cases were positive for fungi. The DNA sequence analysis of six positive specimens which were randomly selected revealed fungi as Candida albicans (n=3), Candida guilliermondii (n=2) and Monilia sp. (n=1). CONCLUSION: A significant association between the presence of fungi in atherosclerotic plaques and severity of atherogenesis and atherosclerotic disease was not found. This could be due to limited numbers of patients included in our study. However, the presence of fungal elements in 26.8% of our specimens is considerable and the results does not exclude the correlation between the presence of fungi with atherosclerosis and coronary artery disease. Tehran University of Medical Sciences 2015-08 /pmc/articles/PMC4645732/ /pubmed/26587476 Text en Copyright© Iranian Public Health Association & Tehran University of Medical Sciences This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
MASOUMI, Omid
SHAHZADI, Mahmoud
KORDBACHEH, Parivash
ZAINI, Farideh
MAHMOUDI, Shahram
MAHMOUDI, Mahmoud
BAHREINI, Hesamoddin
SAFARA, Mahin
MIRHENDI, Hossein
Detection of Fungal Elements in Atherosclerotic Plaques Using Mycological, Pathological and Molecular Methods
title Detection of Fungal Elements in Atherosclerotic Plaques Using Mycological, Pathological and Molecular Methods
title_full Detection of Fungal Elements in Atherosclerotic Plaques Using Mycological, Pathological and Molecular Methods
title_fullStr Detection of Fungal Elements in Atherosclerotic Plaques Using Mycological, Pathological and Molecular Methods
title_full_unstemmed Detection of Fungal Elements in Atherosclerotic Plaques Using Mycological, Pathological and Molecular Methods
title_short Detection of Fungal Elements in Atherosclerotic Plaques Using Mycological, Pathological and Molecular Methods
title_sort detection of fungal elements in atherosclerotic plaques using mycological, pathological and molecular methods
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4645732/
https://www.ncbi.nlm.nih.gov/pubmed/26587476
work_keys_str_mv AT masoumiomid detectionoffungalelementsinatheroscleroticplaquesusingmycologicalpathologicalandmolecularmethods
AT shahzadimahmoud detectionoffungalelementsinatheroscleroticplaquesusingmycologicalpathologicalandmolecularmethods
AT kordbachehparivash detectionoffungalelementsinatheroscleroticplaquesusingmycologicalpathologicalandmolecularmethods
AT zainifarideh detectionoffungalelementsinatheroscleroticplaquesusingmycologicalpathologicalandmolecularmethods
AT mahmoudishahram detectionoffungalelementsinatheroscleroticplaquesusingmycologicalpathologicalandmolecularmethods
AT mahmoudimahmoud detectionoffungalelementsinatheroscleroticplaquesusingmycologicalpathologicalandmolecularmethods
AT bahreinihesamoddin detectionoffungalelementsinatheroscleroticplaquesusingmycologicalpathologicalandmolecularmethods
AT safaramahin detectionoffungalelementsinatheroscleroticplaquesusingmycologicalpathologicalandmolecularmethods
AT mirhendihossein detectionoffungalelementsinatheroscleroticplaquesusingmycologicalpathologicalandmolecularmethods