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Molecular Diversity of Candida albicans Isolated from Immunocompromised Patients, Based on MLST Method
BACKGROUND: As regards multilocus sequence typing (MLST) method directly analyze the polymorphism within DNA sequences; we performed the first nationwide study on the genotypic relationships of Candida albicans strains obtained from oropharynx and bronchoalveolar lavage (BAL) samples from immunocomp...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tehran University of Medical Sciences
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4645784/ https://www.ncbi.nlm.nih.gov/pubmed/26587501 |
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author | AFSARIAN, Seyed Mohammad Hosein BADALI, Hamid SHOKOHI, Tahereh NAJAFIPOUR, Sohrab |
author_facet | AFSARIAN, Seyed Mohammad Hosein BADALI, Hamid SHOKOHI, Tahereh NAJAFIPOUR, Sohrab |
author_sort | AFSARIAN, Seyed Mohammad Hosein |
collection | PubMed |
description | BACKGROUND: As regards multilocus sequence typing (MLST) method directly analyze the polymorphism within DNA sequences; we performed the first nationwide study on the genotypic relationships of Candida albicans strains obtained from oropharynx and bronchoalveolar lavage (BAL) samples from immunocompromised patients. METHODS: Fourteen epidemiologically unrelated clinical strains of C. albicans were obtained from three hospitals in Mazandaran Province, Iran (2006 to 2012) from seven patients with pulmonary infections and the rest with oropharyngeal samples of immunocompromised patients. Seven loci of housekeeping genes were sequenced for all fourteen isolates. RESULTS: MLST was applied to a subset of 14 unrelated isolates. Seventy-one (2.5%) nucleotide sites were found to be variable. Accordingly, 60 different alleles were identified in seven loci among the isolates, among which two new alleles were obtained. Furthermore, 12 independent diploid sequence types (DSTs) including five novel DSTs were identified. The fourteen unrelated isolates were placed in 10 clonal clusters (CC) while two isolates were singletons, by eBURST analysis. Most of the isolates belonged to CC461 of eBURST analysis from the clade 11 and two isolates assigned to CC172 from the clade 15. CONCLUSION: Pathogen distribution and relatedness for determining the epidemiology of nosocomial infections is highly recommended for pathogen control methods. |
format | Online Article Text |
id | pubmed-4645784 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Tehran University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-46457842015-11-19 Molecular Diversity of Candida albicans Isolated from Immunocompromised Patients, Based on MLST Method AFSARIAN, Seyed Mohammad Hosein BADALI, Hamid SHOKOHI, Tahereh NAJAFIPOUR, Sohrab Iran J Public Health Original Article BACKGROUND: As regards multilocus sequence typing (MLST) method directly analyze the polymorphism within DNA sequences; we performed the first nationwide study on the genotypic relationships of Candida albicans strains obtained from oropharynx and bronchoalveolar lavage (BAL) samples from immunocompromised patients. METHODS: Fourteen epidemiologically unrelated clinical strains of C. albicans were obtained from three hospitals in Mazandaran Province, Iran (2006 to 2012) from seven patients with pulmonary infections and the rest with oropharyngeal samples of immunocompromised patients. Seven loci of housekeeping genes were sequenced for all fourteen isolates. RESULTS: MLST was applied to a subset of 14 unrelated isolates. Seventy-one (2.5%) nucleotide sites were found to be variable. Accordingly, 60 different alleles were identified in seven loci among the isolates, among which two new alleles were obtained. Furthermore, 12 independent diploid sequence types (DSTs) including five novel DSTs were identified. The fourteen unrelated isolates were placed in 10 clonal clusters (CC) while two isolates were singletons, by eBURST analysis. Most of the isolates belonged to CC461 of eBURST analysis from the clade 11 and two isolates assigned to CC172 from the clade 15. CONCLUSION: Pathogen distribution and relatedness for determining the epidemiology of nosocomial infections is highly recommended for pathogen control methods. Tehran University of Medical Sciences 2015-09 /pmc/articles/PMC4645784/ /pubmed/26587501 Text en Copyright© Iranian Public Health Association & Tehran University of Medical Sciences This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly. |
spellingShingle | Original Article AFSARIAN, Seyed Mohammad Hosein BADALI, Hamid SHOKOHI, Tahereh NAJAFIPOUR, Sohrab Molecular Diversity of Candida albicans Isolated from Immunocompromised Patients, Based on MLST Method |
title | Molecular Diversity of Candida albicans Isolated from Immunocompromised Patients, Based on MLST Method |
title_full | Molecular Diversity of Candida albicans Isolated from Immunocompromised Patients, Based on MLST Method |
title_fullStr | Molecular Diversity of Candida albicans Isolated from Immunocompromised Patients, Based on MLST Method |
title_full_unstemmed | Molecular Diversity of Candida albicans Isolated from Immunocompromised Patients, Based on MLST Method |
title_short | Molecular Diversity of Candida albicans Isolated from Immunocompromised Patients, Based on MLST Method |
title_sort | molecular diversity of candida albicans isolated from immunocompromised patients, based on mlst method |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4645784/ https://www.ncbi.nlm.nih.gov/pubmed/26587501 |
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