Cargando…

Analytical Sensitivity Comparison between Singleplex Real-Time PCR and a Multiplex PCR Platform for Detecting Respiratory Viruses

Multiplex PCR methods are attractive to clinical laboratories wanting to broaden their detection of respiratory viral pathogens in clinical specimens. However, multiplexed assays must be well optimized to retain or improve upon the analytic sensitivity of their singleplex counterparts. In this exper...

Descripción completa

Detalles Bibliográficos
Autores principales:	Parker, Jayme, Fowler, Nisha, Walmsley, Mary Louise, Schmidt, Terri, Scharrer, Jason, Kowaleski, James, Grimes, Teresa, Hoyos, Shanann, Chen, Jack
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4646456/ https://www.ncbi.nlm.nih.gov/pubmed/26569120 http://dx.doi.org/10.1371/journal.pone.0143164

Ejemplares similares

Correction: Analytical Sensitivity Comparison between Singleplex Real-Time PCR and a Multiplex PCR Platform for Detecting Respiratory Viruses
por: Parker, Jayme, et al.
Publicado: (2018)

685. Comparison of Singleplex qPCR and the Luminex MAGPIX Platform for the Detection of Viral Pathogens
por: Yepsen, Erin, et al.
Publicado: (2020)

Endpoint PCR coupled with capillary electrophoresis (celPCR) provides sensitive and quantitative measures of environmental DNA in singleplex and multiplex reactions
por: Thalinger, Bettina, et al.
Publicado: (2021)

Universal detection of phytoplasmas and Xylella spp. by TaqMan singleplex and multiplex real-time PCR with dual priming oligonucleotides
por: Ito, Takao, et al.
Publicado: (2017)

Optimisation of robust singleplex and multiplex droplet digital PCR assays for high confidence mutation detection in circulating tumour DNA
por: Rowlands, Vicky, et al.
Publicado: (2019)

The use of singleplex and nested PCR to detect Batrachochytrium dendrobatidis in free-living frogs
por: Coutinho, Selene Dall'Acqua, et al.
Publicado: (2015)

Empirical evaluation of humpback whale telomere length estimates; quality control and factors causing variability in the singleplex and multiplex qPCR methods
por: Olsen, Morten Tange, et al.
Publicado: (2012)

Singleplex, multiplex and pooled sample real-time RT-PCR assays for detection of SARS-CoV-2 in an occupational medicine setting
por: Butler, Kimberly S., et al.
Publicado: (2022)

Differential Requirements of Singleplex and Multiplex Recombineering of Large DNA Constructs
por: Reddy, Thimma R., et al.
Publicado: (2015)

Comparison of a singleplex real‐time RT‐PCR assay and multiplex respiratory viral panel assay for detection of influenza “A” in respiratory specimens
por: Pabbaraju, Kanti, et al.
Publicado: (2010)

Comparison of molecular and extract-based allergy diagnostics with multiplex and singleplex analysis
por: Huss-Marp, Johannes, et al.
Publicado: (2015)

Comparison of fast-track diagnostics respiratory pathogens multiplex real-time RT-PCR assay with in-house singleplex assays for comprehensive detection of human respiratory viruses()
por: Sakthivel, Senthilkumar K., et al.
Publicado: (2012)

Development of a Singleplex Real-Time Reverse Transcriptase PCR Assay for Pan-Dengue Virus Detection and Quantification
por: Songjaeng, Adisak, et al.
Publicado: (2022)

A Multi-Label Predictor for Identifying the Subcellular Locations of Singleplex and Multiplex Eukaryotic Proteins
por: Wang, Xiao, et al.
Publicado: (2012)

Evaluation of two singleplex reverse transcription-Insulated isothermal PCR tests and a duplex real-time RT-PCR test for the detection of porcine epidemic diarrhea virus and porcine deltacoronavirus
por: Zhang, Jianqiang, et al.
Publicado: (2016)

Clinical utility of immunological methods based on the singleplex and multiplex ImmunoCap systems for diagnosis of shrimp allergy
por: Ukleja-Sokołowska, Natalia, et al.
Publicado: (2021)

Analytical sensitivity of a multiplex quantitative PCR for Toxoplasma gondii and Neospora caninum
por: Truong, Marcus, et al.
Publicado: (2023)

iLoc-Euk: A Multi-Label Classifier for Predicting the Subcellular Localization of Singleplex and Multiplex Eukaryotic Proteins
por: Chou, Kuo-Chen, et al.
Publicado: (2011)

Fundamentals of multiplexing with digital PCR
por: Whale, Alexandra S., et al.
Publicado: (2016)

Highly multiplexed quantitative PCR-based platform for evaluation of chicken immune responses
por: Borowska, Dominika, et al.
Publicado: (2019)

Multiplex PCR in Molecular Differential Diagnosis of Microbial Infections: Methods, Utility, and Platforms
por: Han, Jian
Publicado: (2012)

IgE multiplex testing in house dust mite allergy is utile, and sensitivity is comparable to extract‐based singleplex testing
por: Koch, Lukas, et al.
Publicado: (2020)

Applicability of a duplex and four singleplex real-time PCR assays for the qualitative and quantitative determination of wild boar and domestic pig meat in processed food products
por: Kaltenbrunner, Maria, et al.
Publicado: (2020)

Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
por: O'Sullivan, Matthew V. N., et al.
Publicado: (2011)

Validation of Multiplex PCR and Serology Detecting Helicobacter Species in Mice
por: Butt, Julia, et al.
Publicado: (2023)

Development of a Multiplex PCR Platform for the Rapid Detection of Bacteria, Antibiotic Resistance, and Candida in Human Blood Samples
por: Liotti, Flora Marzia, et al.
Publicado: (2019)

Singleplex real-time RT-PCR for detection of influenza A virus and simultaneous differentiation of A/H1N1v and evaluation of the RealStar influenza kit
por: Panning, Marcus, et al.
Publicado: (2011)

Multiplexed-tandem PCR (MT-PCR) assay to detect and differentiate gastrointestinal nematodes of alpacas
por: Rashid, Mohammed H., et al.
Publicado: (2018)

Nested-RT-PCR and Multiplex RT-PCR for Diagnosis of Rhinovirus Infection in Clinical Samples
por: Yu, Xuelian, et al.
Publicado: (2014)

DNA analysis with multiplex microarray-enhanced PCR
por: Pemov, A., et al.
Publicado: (2005)

Multiplex PCR for Rapid Diagnosis of Gastrointestinal Tuberculosis
por: Sharma, Kusum, et al.
Publicado: (2013)

Validation of multiplex PCR for detection and differentiation of Salmonellas
por: Stegniy, Borys, et al.
Publicado: (2015)

Highly multiplexed single-cell quantitative PCR
por: VanInsberghe, Michael, et al.
Publicado: (2018)

Development of multiplex PCR for neglected infectious diseases
por: Sea-liang, Nutchanart, et al.
Publicado: (2019)

Molecular Detection of the mcr Genes by Multiplex PCR
por: Liu, Jiayang, et al.
Publicado: (2020)

Analysis of multiplex PCR fragments with PMMA microchip
por: Liu, Dayu, et al.
Publicado: (2006)

Multiplex PCR for Detection and Identification of Microbial Pathogens
por: Poritz, Mark A., et al.
Publicado: (2018)

Validation of multiplex PCR sequencing assay of SIV
por: Moriarty, Ryan V., et al.
Publicado: (2021)

Multiplex detection of “Candidatus Liberibacter asiaticus” and Spiroplasma citri by qPCR and droplet digital PCR
por: Maheshwari, Yogita, et al.
Publicado: (2021)

primerJinn – a tool for rationally designing multiplex PCR primer sets and in silico PCR
por: Limberis, Jason D, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_mcj6ioqun3us6eqq80qrudhll9