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Gas Chromatography-Mass Spectrometry Analysis of Ulva fasciata (Green Seaweed) Extract and Evaluation of Its Cytoprotective and Antigenotoxic Effects

The chemical composition and biological properties of Ulva fasciata aqueous-ethanolic extract were examined. Five components were identified in one fraction prepared from the extract by gas chromatography-mass spectrometry, and palmitic acid and its ethyl ester accounted for 76% of the total identif...

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Autores principales: Rodeiro, Idania, Olguín, Sitlali, Santes, Rebeca, Herrera, José A., Pérez, Carlos L., Mangas, Raisa, Hernández, Yasnay, Fernández, Gisselle, Hernández, Ivones, Hernández-Ojeda, Sandra, Camacho-Carranza, Rafael, Valencia-Olvera, Ana, Espinosa-Aguirre, Jesús Javier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647032/
https://www.ncbi.nlm.nih.gov/pubmed/26612994
http://dx.doi.org/10.1155/2015/520598
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author Rodeiro, Idania
Olguín, Sitlali
Santes, Rebeca
Herrera, José A.
Pérez, Carlos L.
Mangas, Raisa
Hernández, Yasnay
Fernández, Gisselle
Hernández, Ivones
Hernández-Ojeda, Sandra
Camacho-Carranza, Rafael
Valencia-Olvera, Ana
Espinosa-Aguirre, Jesús Javier
author_facet Rodeiro, Idania
Olguín, Sitlali
Santes, Rebeca
Herrera, José A.
Pérez, Carlos L.
Mangas, Raisa
Hernández, Yasnay
Fernández, Gisselle
Hernández, Ivones
Hernández-Ojeda, Sandra
Camacho-Carranza, Rafael
Valencia-Olvera, Ana
Espinosa-Aguirre, Jesús Javier
author_sort Rodeiro, Idania
collection PubMed
description The chemical composition and biological properties of Ulva fasciata aqueous-ethanolic extract were examined. Five components were identified in one fraction prepared from the extract by gas chromatography-mass spectrometry, and palmitic acid and its ethyl ester accounted for 76% of the total identified components. Furthermore, we assessed the extract's antioxidant properties by using the DPPH, ABTS, and lipid peroxidation assays and found that the extract had a moderate scavenging effect. In an experiment involving preexposition and coexposition of the extract (1–500 µg/mL) and benzo[a]pyrene (BP), the extract was found to be nontoxic to C9 cells in culture and to inhibit the cytotoxicity induced by BP. As BP is biotransformed by CYP1A and CYP2B subfamilies, we explored the possible interaction of the extract with these enzymes. The extract (25–50 µg/mL) inhibited CYP1A1 activity in rat liver microsomes. Analysis of the inhibition kinetics revealed a mixed-type inhibitory effect on CYP1A1 supersome. The effects of the extract on BP-induced DNA damage and hepatic CYP activity in mice were also investigated. Micronuclei induction by BP and liver CYP1A1/2 activities significantly decreased in animals treated with the extract. The results suggest that Ulva fasciata aqueous-ethanolic extract inhibits BP bioactivation and it may be a potential chemopreventive agent.
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spelling pubmed-46470322015-11-26 Gas Chromatography-Mass Spectrometry Analysis of Ulva fasciata (Green Seaweed) Extract and Evaluation of Its Cytoprotective and Antigenotoxic Effects Rodeiro, Idania Olguín, Sitlali Santes, Rebeca Herrera, José A. Pérez, Carlos L. Mangas, Raisa Hernández, Yasnay Fernández, Gisselle Hernández, Ivones Hernández-Ojeda, Sandra Camacho-Carranza, Rafael Valencia-Olvera, Ana Espinosa-Aguirre, Jesús Javier Evid Based Complement Alternat Med Research Article The chemical composition and biological properties of Ulva fasciata aqueous-ethanolic extract were examined. Five components were identified in one fraction prepared from the extract by gas chromatography-mass spectrometry, and palmitic acid and its ethyl ester accounted for 76% of the total identified components. Furthermore, we assessed the extract's antioxidant properties by using the DPPH, ABTS, and lipid peroxidation assays and found that the extract had a moderate scavenging effect. In an experiment involving preexposition and coexposition of the extract (1–500 µg/mL) and benzo[a]pyrene (BP), the extract was found to be nontoxic to C9 cells in culture and to inhibit the cytotoxicity induced by BP. As BP is biotransformed by CYP1A and CYP2B subfamilies, we explored the possible interaction of the extract with these enzymes. The extract (25–50 µg/mL) inhibited CYP1A1 activity in rat liver microsomes. Analysis of the inhibition kinetics revealed a mixed-type inhibitory effect on CYP1A1 supersome. The effects of the extract on BP-induced DNA damage and hepatic CYP activity in mice were also investigated. Micronuclei induction by BP and liver CYP1A1/2 activities significantly decreased in animals treated with the extract. The results suggest that Ulva fasciata aqueous-ethanolic extract inhibits BP bioactivation and it may be a potential chemopreventive agent. Hindawi Publishing Corporation 2015 2015-11-03 /pmc/articles/PMC4647032/ /pubmed/26612994 http://dx.doi.org/10.1155/2015/520598 Text en Copyright © 2015 Idania Rodeiro et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Rodeiro, Idania
Olguín, Sitlali
Santes, Rebeca
Herrera, José A.
Pérez, Carlos L.
Mangas, Raisa
Hernández, Yasnay
Fernández, Gisselle
Hernández, Ivones
Hernández-Ojeda, Sandra
Camacho-Carranza, Rafael
Valencia-Olvera, Ana
Espinosa-Aguirre, Jesús Javier
Gas Chromatography-Mass Spectrometry Analysis of Ulva fasciata (Green Seaweed) Extract and Evaluation of Its Cytoprotective and Antigenotoxic Effects
title Gas Chromatography-Mass Spectrometry Analysis of Ulva fasciata (Green Seaweed) Extract and Evaluation of Its Cytoprotective and Antigenotoxic Effects
title_full Gas Chromatography-Mass Spectrometry Analysis of Ulva fasciata (Green Seaweed) Extract and Evaluation of Its Cytoprotective and Antigenotoxic Effects
title_fullStr Gas Chromatography-Mass Spectrometry Analysis of Ulva fasciata (Green Seaweed) Extract and Evaluation of Its Cytoprotective and Antigenotoxic Effects
title_full_unstemmed Gas Chromatography-Mass Spectrometry Analysis of Ulva fasciata (Green Seaweed) Extract and Evaluation of Its Cytoprotective and Antigenotoxic Effects
title_short Gas Chromatography-Mass Spectrometry Analysis of Ulva fasciata (Green Seaweed) Extract and Evaluation of Its Cytoprotective and Antigenotoxic Effects
title_sort gas chromatography-mass spectrometry analysis of ulva fasciata (green seaweed) extract and evaluation of its cytoprotective and antigenotoxic effects
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647032/
https://www.ncbi.nlm.nih.gov/pubmed/26612994
http://dx.doi.org/10.1155/2015/520598
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