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Nr2f1b control venous specification and angiogenic patterning during zebrafish vascular development
BACKGROUND: The specification of vein and the patterning of intersegmental vessels (ISV) controlled by transcription factor is not fully characterized. The orphan nuclear receptor Chicken ovalbumin upstream promoter transcription factor II (CoupTFII, a.k.a NR2F2) positively regulates vein identity i...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647328/ https://www.ncbi.nlm.nih.gov/pubmed/26572615 http://dx.doi.org/10.1186/s12929-015-0209-0 |
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author | Li, Ru-Fang Wu, Ting-Yun Mou, Yu-Zheng Wang, Yi-Shan Chen, Chun-Lin Wu, Chang-Yi |
author_facet | Li, Ru-Fang Wu, Ting-Yun Mou, Yu-Zheng Wang, Yi-Shan Chen, Chun-Lin Wu, Chang-Yi |
author_sort | Li, Ru-Fang |
collection | PubMed |
description | BACKGROUND: The specification of vein and the patterning of intersegmental vessels (ISV) controlled by transcription factor is not fully characterized. The orphan nuclear receptor Chicken ovalbumin upstream promoter transcription factor II (CoupTFII, a.k.a NR2F2) positively regulates vein identity in mice. In this study, we show that nr2f1b is important for vein and tip cell identity during zebrafish development. RESULTS: Nr2f1b mRNA is expressed in ventral lateral mesoderm at 15S stage and in vessels at 24 hpf consistent with a role in early vascular specification. Morpholino knockdown of nr2f1b results in a decrease in both vein cell number and expression of the vein specific marker flt4 and mrc1, suggested its role in venous specification. We also show loss of nr2f1b reduced ISV cell number and impairs ISV growth, which is likely due to the impairment of angiogenic cells migration and/or proliferation by time-lapse imaging. Consequently, nr2f1b morphants showed pericardial edema and circulation defects. Overexpression of nr2f1b under the fli promoter increases the number of venous cells and ISV endothelial cells indicated the function of nr2f1b is required and necessary for vascular development. We further showed that nr2f1b likely interact with Notch signalling. nr2f1b expression is increased in rbpsuh morphants and DAPT-treatment embryos suggested nr2f1b is negatively regulated by Notch activity. CONCLUSIONS: We show nr2f1b control venous specification and angiogenic patterning during zebrafish vascular development, which is mediated by Notch signalings. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12929-015-0209-0) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4647328 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-46473282015-11-18 Nr2f1b control venous specification and angiogenic patterning during zebrafish vascular development Li, Ru-Fang Wu, Ting-Yun Mou, Yu-Zheng Wang, Yi-Shan Chen, Chun-Lin Wu, Chang-Yi J Biomed Sci Research BACKGROUND: The specification of vein and the patterning of intersegmental vessels (ISV) controlled by transcription factor is not fully characterized. The orphan nuclear receptor Chicken ovalbumin upstream promoter transcription factor II (CoupTFII, a.k.a NR2F2) positively regulates vein identity in mice. In this study, we show that nr2f1b is important for vein and tip cell identity during zebrafish development. RESULTS: Nr2f1b mRNA is expressed in ventral lateral mesoderm at 15S stage and in vessels at 24 hpf consistent with a role in early vascular specification. Morpholino knockdown of nr2f1b results in a decrease in both vein cell number and expression of the vein specific marker flt4 and mrc1, suggested its role in venous specification. We also show loss of nr2f1b reduced ISV cell number and impairs ISV growth, which is likely due to the impairment of angiogenic cells migration and/or proliferation by time-lapse imaging. Consequently, nr2f1b morphants showed pericardial edema and circulation defects. Overexpression of nr2f1b under the fli promoter increases the number of venous cells and ISV endothelial cells indicated the function of nr2f1b is required and necessary for vascular development. We further showed that nr2f1b likely interact with Notch signalling. nr2f1b expression is increased in rbpsuh morphants and DAPT-treatment embryos suggested nr2f1b is negatively regulated by Notch activity. CONCLUSIONS: We show nr2f1b control venous specification and angiogenic patterning during zebrafish vascular development, which is mediated by Notch signalings. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12929-015-0209-0) contains supplementary material, which is available to authorized users. BioMed Central 2015-11-17 /pmc/articles/PMC4647328/ /pubmed/26572615 http://dx.doi.org/10.1186/s12929-015-0209-0 Text en © Li et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Li, Ru-Fang Wu, Ting-Yun Mou, Yu-Zheng Wang, Yi-Shan Chen, Chun-Lin Wu, Chang-Yi Nr2f1b control venous specification and angiogenic patterning during zebrafish vascular development |
title | Nr2f1b control venous specification and angiogenic patterning during zebrafish vascular development |
title_full | Nr2f1b control venous specification and angiogenic patterning during zebrafish vascular development |
title_fullStr | Nr2f1b control venous specification and angiogenic patterning during zebrafish vascular development |
title_full_unstemmed | Nr2f1b control venous specification and angiogenic patterning during zebrafish vascular development |
title_short | Nr2f1b control venous specification and angiogenic patterning during zebrafish vascular development |
title_sort | nr2f1b control venous specification and angiogenic patterning during zebrafish vascular development |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647328/ https://www.ncbi.nlm.nih.gov/pubmed/26572615 http://dx.doi.org/10.1186/s12929-015-0209-0 |
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