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An S6:S18 complex inhibits translation of E. coli rpsF
More than half of the ribosomal protein operons in Escherichia coli are regulated by structures within the mRNA transcripts that interact with specific ribosomal proteins to inhibit further protein expression. This regulation is accomplished using a variety of mechanisms and the RNA structures respo...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647458/ https://www.ncbi.nlm.nih.gov/pubmed/26447183 http://dx.doi.org/10.1261/rna.049544.115 |
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author | Babina, Arianne M. Soo, Mark W. Fu, Yang Meyer, Michelle M. |
author_facet | Babina, Arianne M. Soo, Mark W. Fu, Yang Meyer, Michelle M. |
author_sort | Babina, Arianne M. |
collection | PubMed |
description | More than half of the ribosomal protein operons in Escherichia coli are regulated by structures within the mRNA transcripts that interact with specific ribosomal proteins to inhibit further protein expression. This regulation is accomplished using a variety of mechanisms and the RNA structures responsible for regulation are often not conserved across bacterial phyla. A widely conserved mRNA structure preceding the ribosomal protein operon containing rpsF and rpsR (encoding S6 and S18) was recently identified through comparative genomics. Examples of this RNA from both E. coli and Bacillus subtilis were shown to interact in vitro with an S6:S18 complex. In this work, we demonstrate that in E. coli, this RNA structure regulates gene expression in response to the S6:S18 complex. β-galactosidase activity from a lacZ reporter translationally fused to the 5′ UTR and first nine codons of E. coli rpsF is reduced fourfold by overexpression of a genomic fragment encoding both S6 and S18 but not by overexpression of either protein individually. Mutations to the mRNA structure, as well as to the RNA-binding site of S18 and the S6–S18 interaction surfaces of S6 and S18, are sufficient to derepress β-galactosidase activity, indicating that the S6:S18 complex is the biologically active effector. Measurement of transcript levels shows that although reporter levels do not change upon protein overexpression, levels of the native transcript are reduced fourfold, suggesting that the mRNA regulator prevents translation and this effect is amplified on the native transcript by other mechanisms. |
format | Online Article Text |
id | pubmed-4647458 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-46474582015-12-01 An S6:S18 complex inhibits translation of E. coli rpsF Babina, Arianne M. Soo, Mark W. Fu, Yang Meyer, Michelle M. RNA Report More than half of the ribosomal protein operons in Escherichia coli are regulated by structures within the mRNA transcripts that interact with specific ribosomal proteins to inhibit further protein expression. This regulation is accomplished using a variety of mechanisms and the RNA structures responsible for regulation are often not conserved across bacterial phyla. A widely conserved mRNA structure preceding the ribosomal protein operon containing rpsF and rpsR (encoding S6 and S18) was recently identified through comparative genomics. Examples of this RNA from both E. coli and Bacillus subtilis were shown to interact in vitro with an S6:S18 complex. In this work, we demonstrate that in E. coli, this RNA structure regulates gene expression in response to the S6:S18 complex. β-galactosidase activity from a lacZ reporter translationally fused to the 5′ UTR and first nine codons of E. coli rpsF is reduced fourfold by overexpression of a genomic fragment encoding both S6 and S18 but not by overexpression of either protein individually. Mutations to the mRNA structure, as well as to the RNA-binding site of S18 and the S6–S18 interaction surfaces of S6 and S18, are sufficient to derepress β-galactosidase activity, indicating that the S6:S18 complex is the biologically active effector. Measurement of transcript levels shows that although reporter levels do not change upon protein overexpression, levels of the native transcript are reduced fourfold, suggesting that the mRNA regulator prevents translation and this effect is amplified on the native transcript by other mechanisms. Cold Spring Harbor Laboratory Press 2015-12 /pmc/articles/PMC4647458/ /pubmed/26447183 http://dx.doi.org/10.1261/rna.049544.115 Text en © 2015 Babina et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by/4.0/ This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Report Babina, Arianne M. Soo, Mark W. Fu, Yang Meyer, Michelle M. An S6:S18 complex inhibits translation of E. coli rpsF |
title | An S6:S18 complex inhibits translation of E. coli rpsF |
title_full | An S6:S18 complex inhibits translation of E. coli rpsF |
title_fullStr | An S6:S18 complex inhibits translation of E. coli rpsF |
title_full_unstemmed | An S6:S18 complex inhibits translation of E. coli rpsF |
title_short | An S6:S18 complex inhibits translation of E. coli rpsF |
title_sort | s6:s18 complex inhibits translation of e. coli rpsf |
topic | Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647458/ https://www.ncbi.nlm.nih.gov/pubmed/26447183 http://dx.doi.org/10.1261/rna.049544.115 |
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