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Properties of short double-stranded RNAs carrying randomized base pairs: toward better controls for RNAi experiments

Short interfering RNAs (siRNAs) are mediators of RNA interference (RNAi), a commonly used technique for selective down-regulation of target gene expression. Using an equimolar mixture of A, G, C, and U phosphoramidites during solid-phase synthesis, we introduced degenerate positions into RNA guide a...

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Detalles Bibliográficos
Autores principales:	Zagalak, Julian A., Menzi, Mirjam, Schmich, Fabian, Jahns, Hartmut, Dogar, Afzal M., Wullschleger, Florian, Towbin, Harry, Hall, Jonathan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cold Spring Harbor Laboratory Press 2015
Materias:	Method
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647466/ https://www.ncbi.nlm.nih.gov/pubmed/26516083 http://dx.doi.org/10.1261/rna.053637.115

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