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Genome-wide analysis and expression profiling of the PIN auxin transporter gene family in soybean (Glycine max)

BACKGROUND: The plant phytohormone auxin controls many aspects of plant growth and development, which largely depends on its uneven distribution in plant tissues. Transmembrane proteins of the PIN family are auxin efflux facilitators. They play a key role in polar auxin transport and are associated...

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Autores principales: Wang, Yongqin, Chai, Chenglin, Valliyodan, Babu, Maupin, Christine, Annen, Brad, Nguyen, Henry T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647520/
https://www.ncbi.nlm.nih.gov/pubmed/26572792
http://dx.doi.org/10.1186/s12864-015-2149-1
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author Wang, Yongqin
Chai, Chenglin
Valliyodan, Babu
Maupin, Christine
Annen, Brad
Nguyen, Henry T.
author_facet Wang, Yongqin
Chai, Chenglin
Valliyodan, Babu
Maupin, Christine
Annen, Brad
Nguyen, Henry T.
author_sort Wang, Yongqin
collection PubMed
description BACKGROUND: The plant phytohormone auxin controls many aspects of plant growth and development, which largely depends on its uneven distribution in plant tissues. Transmembrane proteins of the PIN family are auxin efflux facilitators. They play a key role in polar auxin transport and are associated with auxin asymmetrical distribution in plants. PIN genes have been characterized in several plant species, while comprehensive analysis of this gene family in soybean has not been reported yet. RESULTS: In this study, twenty-three members of the PIN gene family were identified in the soybean genome through homology searches. Analysis of chromosome distribution and phylogenetic relationships of the soybean PIN genes indicated nine pairs of duplicated genes and a legume specific subfamily. Organ/tissue expression patterns and promoter activity assays of the soybean PINs suggested redundant functions for most duplicated genes and complementary and tissue-specific functions during development for non-duplicated genes. The soybean PIN genes were differentially regulated by various abiotic stresses and phytohormone stimuli, implying crosstalk between auxin and abiotic stress signaling pathways. This was further supported by the altered auxin distribution under these conditions as revealed by DR5::GUS transgenic soybean hairy root. Our data indicates that GmPIN9, a legume-specific PIN gene, which was responsive to several abiotic stresses, might play a role in auxin re-distribution in soybean root under abiotic stress conditions. CONCLUSIONS: This study provided the first comprehensive analysis of the soybean PIN gene family. Information on phylogenetic relationships, gene structure, protein profiles and expression profiles of the soybean PIN genes in different tissues and under various abiotic stress treatments helps to identity candidates with potential roles in specific developmental processes and/or environmental stress conditions. Our study advances our understanding of plant responses to abiotic stresses and serves as a basis for uncovering the biological role of PIN genes in soybean development and adaption to adverse environments. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-015-2149-1) contains supplementary material, which is available to authorized users.
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spelling pubmed-46475202015-11-18 Genome-wide analysis and expression profiling of the PIN auxin transporter gene family in soybean (Glycine max) Wang, Yongqin Chai, Chenglin Valliyodan, Babu Maupin, Christine Annen, Brad Nguyen, Henry T. BMC Genomics Research Article BACKGROUND: The plant phytohormone auxin controls many aspects of plant growth and development, which largely depends on its uneven distribution in plant tissues. Transmembrane proteins of the PIN family are auxin efflux facilitators. They play a key role in polar auxin transport and are associated with auxin asymmetrical distribution in plants. PIN genes have been characterized in several plant species, while comprehensive analysis of this gene family in soybean has not been reported yet. RESULTS: In this study, twenty-three members of the PIN gene family were identified in the soybean genome through homology searches. Analysis of chromosome distribution and phylogenetic relationships of the soybean PIN genes indicated nine pairs of duplicated genes and a legume specific subfamily. Organ/tissue expression patterns and promoter activity assays of the soybean PINs suggested redundant functions for most duplicated genes and complementary and tissue-specific functions during development for non-duplicated genes. The soybean PIN genes were differentially regulated by various abiotic stresses and phytohormone stimuli, implying crosstalk between auxin and abiotic stress signaling pathways. This was further supported by the altered auxin distribution under these conditions as revealed by DR5::GUS transgenic soybean hairy root. Our data indicates that GmPIN9, a legume-specific PIN gene, which was responsive to several abiotic stresses, might play a role in auxin re-distribution in soybean root under abiotic stress conditions. CONCLUSIONS: This study provided the first comprehensive analysis of the soybean PIN gene family. Information on phylogenetic relationships, gene structure, protein profiles and expression profiles of the soybean PIN genes in different tissues and under various abiotic stress treatments helps to identity candidates with potential roles in specific developmental processes and/or environmental stress conditions. Our study advances our understanding of plant responses to abiotic stresses and serves as a basis for uncovering the biological role of PIN genes in soybean development and adaption to adverse environments. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-015-2149-1) contains supplementary material, which is available to authorized users. BioMed Central 2015-11-16 /pmc/articles/PMC4647520/ /pubmed/26572792 http://dx.doi.org/10.1186/s12864-015-2149-1 Text en © Wang et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Wang, Yongqin
Chai, Chenglin
Valliyodan, Babu
Maupin, Christine
Annen, Brad
Nguyen, Henry T.
Genome-wide analysis and expression profiling of the PIN auxin transporter gene family in soybean (Glycine max)
title Genome-wide analysis and expression profiling of the PIN auxin transporter gene family in soybean (Glycine max)
title_full Genome-wide analysis and expression profiling of the PIN auxin transporter gene family in soybean (Glycine max)
title_fullStr Genome-wide analysis and expression profiling of the PIN auxin transporter gene family in soybean (Glycine max)
title_full_unstemmed Genome-wide analysis and expression profiling of the PIN auxin transporter gene family in soybean (Glycine max)
title_short Genome-wide analysis and expression profiling of the PIN auxin transporter gene family in soybean (Glycine max)
title_sort genome-wide analysis and expression profiling of the pin auxin transporter gene family in soybean (glycine max)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647520/
https://www.ncbi.nlm.nih.gov/pubmed/26572792
http://dx.doi.org/10.1186/s12864-015-2149-1
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