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Bioreactor performance parameters for an industrially-promising methanotroph Methylomicrobium buryatense 5GB1

BACKGROUND: Methane is a feedstock of interest for the future, both from natural gas and from renewable biogas sources. Methanotrophic bacteria have the potential to enable commercial methane bioconversion to value-added products such as fuels and chemicals. A strain of interest for such application...

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Autores principales: Gilman, Alexey, Laurens, Lieve M., Puri, Aaron W., Chu, Frances, Pienkos, Philip T., Lidstrom, Mary E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647623/
https://www.ncbi.nlm.nih.gov/pubmed/26572866
http://dx.doi.org/10.1186/s12934-015-0372-8
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author Gilman, Alexey
Laurens, Lieve M.
Puri, Aaron W.
Chu, Frances
Pienkos, Philip T.
Lidstrom, Mary E.
author_facet Gilman, Alexey
Laurens, Lieve M.
Puri, Aaron W.
Chu, Frances
Pienkos, Philip T.
Lidstrom, Mary E.
author_sort Gilman, Alexey
collection PubMed
description BACKGROUND: Methane is a feedstock of interest for the future, both from natural gas and from renewable biogas sources. Methanotrophic bacteria have the potential to enable commercial methane bioconversion to value-added products such as fuels and chemicals. A strain of interest for such applications is Methylomicrobium buryatense 5GB1, due to its robust growth characteristics. However, to take advantage of the potential of this methanotroph, it is important to generate comprehensive bioreactor-based datasets for different growth conditions to compare bioprocess parameters. RESULTS: Datasets of growth parameters, gas utilization rates, and products (total biomass, extracted fatty acids, glycogen, excreted acids) were obtained for cultures of M. buryatense 5GB1 grown in continuous culture under methane limitation and O(2) limitation conditions. Additionally, experiments were performed involving unrestricted batch growth conditions with both methane and methanol as substrate. All four growth conditions show significant differences. The most notable changes are the high glycogen content and high formate excretion for cells grown on methanol (batch), and high O(2):CH(4) utilization ratio for cells grown under methane limitation. CONCLUSIONS: The results presented here represent the most comprehensive published bioreactor datasets for a gamma-proteobacterial methanotroph. This information shows that metabolism by M. buryatense 5GB1 differs significantly for each of the four conditions tested. O(2) limitation resulted in the lowest relative O(2) demand and fed-batch growth on methane the highest. Future studies are needed to understand the metabolic basis of these differences. However, these results suggest that both batch and continuous culture conditions have specific advantages, depending on the product of interest. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12934-015-0372-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-46476232015-11-18 Bioreactor performance parameters for an industrially-promising methanotroph Methylomicrobium buryatense 5GB1 Gilman, Alexey Laurens, Lieve M. Puri, Aaron W. Chu, Frances Pienkos, Philip T. Lidstrom, Mary E. Microb Cell Fact Research BACKGROUND: Methane is a feedstock of interest for the future, both from natural gas and from renewable biogas sources. Methanotrophic bacteria have the potential to enable commercial methane bioconversion to value-added products such as fuels and chemicals. A strain of interest for such applications is Methylomicrobium buryatense 5GB1, due to its robust growth characteristics. However, to take advantage of the potential of this methanotroph, it is important to generate comprehensive bioreactor-based datasets for different growth conditions to compare bioprocess parameters. RESULTS: Datasets of growth parameters, gas utilization rates, and products (total biomass, extracted fatty acids, glycogen, excreted acids) were obtained for cultures of M. buryatense 5GB1 grown in continuous culture under methane limitation and O(2) limitation conditions. Additionally, experiments were performed involving unrestricted batch growth conditions with both methane and methanol as substrate. All four growth conditions show significant differences. The most notable changes are the high glycogen content and high formate excretion for cells grown on methanol (batch), and high O(2):CH(4) utilization ratio for cells grown under methane limitation. CONCLUSIONS: The results presented here represent the most comprehensive published bioreactor datasets for a gamma-proteobacterial methanotroph. This information shows that metabolism by M. buryatense 5GB1 differs significantly for each of the four conditions tested. O(2) limitation resulted in the lowest relative O(2) demand and fed-batch growth on methane the highest. Future studies are needed to understand the metabolic basis of these differences. However, these results suggest that both batch and continuous culture conditions have specific advantages, depending on the product of interest. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12934-015-0372-8) contains supplementary material, which is available to authorized users. BioMed Central 2015-11-16 /pmc/articles/PMC4647623/ /pubmed/26572866 http://dx.doi.org/10.1186/s12934-015-0372-8 Text en © Gilman et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Gilman, Alexey
Laurens, Lieve M.
Puri, Aaron W.
Chu, Frances
Pienkos, Philip T.
Lidstrom, Mary E.
Bioreactor performance parameters for an industrially-promising methanotroph Methylomicrobium buryatense 5GB1
title Bioreactor performance parameters for an industrially-promising methanotroph Methylomicrobium buryatense 5GB1
title_full Bioreactor performance parameters for an industrially-promising methanotroph Methylomicrobium buryatense 5GB1
title_fullStr Bioreactor performance parameters for an industrially-promising methanotroph Methylomicrobium buryatense 5GB1
title_full_unstemmed Bioreactor performance parameters for an industrially-promising methanotroph Methylomicrobium buryatense 5GB1
title_short Bioreactor performance parameters for an industrially-promising methanotroph Methylomicrobium buryatense 5GB1
title_sort bioreactor performance parameters for an industrially-promising methanotroph methylomicrobium buryatense 5gb1
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647623/
https://www.ncbi.nlm.nih.gov/pubmed/26572866
http://dx.doi.org/10.1186/s12934-015-0372-8
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