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Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes

BACKGROUND: Salvia diterpenes have been found to have health promoting properties. Among them, carnosic acid and carnosol, tanshinones and sclareol are well known for their cardiovascular, antitumor, antiinflammatory and antioxidant activities. However, many of these compounds are not available at a...

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Autores principales: Trikka, Fotini A., Nikolaidis, Alexandros, Ignea, Codruta, Tsaballa, Aphrodite, Tziveleka, Leto-Aikaterini, Ioannou, Efstathia, Roussis, Vassilios, Stea, Eleni A., Božić, Dragana, Argiriou, Anagnostis, Kanellis, Angelos K., Kampranis, Sotirios C., Makris, Antonios M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647624/
https://www.ncbi.nlm.nih.gov/pubmed/26572682
http://dx.doi.org/10.1186/s12864-015-2147-3
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author Trikka, Fotini A.
Nikolaidis, Alexandros
Ignea, Codruta
Tsaballa, Aphrodite
Tziveleka, Leto-Aikaterini
Ioannou, Efstathia
Roussis, Vassilios
Stea, Eleni A.
Božić, Dragana
Argiriou, Anagnostis
Kanellis, Angelos K.
Kampranis, Sotirios C.
Makris, Antonios M.
author_facet Trikka, Fotini A.
Nikolaidis, Alexandros
Ignea, Codruta
Tsaballa, Aphrodite
Tziveleka, Leto-Aikaterini
Ioannou, Efstathia
Roussis, Vassilios
Stea, Eleni A.
Božić, Dragana
Argiriou, Anagnostis
Kanellis, Angelos K.
Kampranis, Sotirios C.
Makris, Antonios M.
author_sort Trikka, Fotini A.
collection PubMed
description BACKGROUND: Salvia diterpenes have been found to have health promoting properties. Among them, carnosic acid and carnosol, tanshinones and sclareol are well known for their cardiovascular, antitumor, antiinflammatory and antioxidant activities. However, many of these compounds are not available at a constant supply and developing biotechnological methods for their production could provide a sustainable alternative. The transcriptome of S.pomifera glandular trichomes was analysed aiming to identify genes that could be used in the engineering of synthetic microbial systems. RESULTS: In the present study, a thorough metabolite analysis of S. pomifera leaves led to the isolation and structure elucidation of carnosic acid-family metabolites including one new natural product. These labdane diterpenes seem to be synthesized through miltiradiene and ferruginol. Transcriptomic analysis of the glandular trichomes from the S. pomifera leaves revealed two genes likely involved in miltiradiene synthesis. Their products were identified and the corresponding enzymes were characterized as copalyl diphosphate synthase (SpCDS) and miltiradiene synthase (SpMilS). In addition, several CYP-encoding transcripts were identified providing a valuable resource for the identification of the biosynthetic mechanism responsible for the production of carnosic acid-family metabolites in S. pomifera. CONCLUSIONS: Our work has uncovered the key enzymes involved in miltiradiene biosynthesis in S. pomifera leaf glandular trichomes. The transcriptomic dataset obtained provides a valuable tool for the identification of the CYPs involved in the synthesis of carnosic acid-family metabolites. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-015-2147-3) contains supplementary material, which is available to authorized users.
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spelling pubmed-46476242015-11-18 Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes Trikka, Fotini A. Nikolaidis, Alexandros Ignea, Codruta Tsaballa, Aphrodite Tziveleka, Leto-Aikaterini Ioannou, Efstathia Roussis, Vassilios Stea, Eleni A. Božić, Dragana Argiriou, Anagnostis Kanellis, Angelos K. Kampranis, Sotirios C. Makris, Antonios M. BMC Genomics Research Article BACKGROUND: Salvia diterpenes have been found to have health promoting properties. Among them, carnosic acid and carnosol, tanshinones and sclareol are well known for their cardiovascular, antitumor, antiinflammatory and antioxidant activities. However, many of these compounds are not available at a constant supply and developing biotechnological methods for their production could provide a sustainable alternative. The transcriptome of S.pomifera glandular trichomes was analysed aiming to identify genes that could be used in the engineering of synthetic microbial systems. RESULTS: In the present study, a thorough metabolite analysis of S. pomifera leaves led to the isolation and structure elucidation of carnosic acid-family metabolites including one new natural product. These labdane diterpenes seem to be synthesized through miltiradiene and ferruginol. Transcriptomic analysis of the glandular trichomes from the S. pomifera leaves revealed two genes likely involved in miltiradiene synthesis. Their products were identified and the corresponding enzymes were characterized as copalyl diphosphate synthase (SpCDS) and miltiradiene synthase (SpMilS). In addition, several CYP-encoding transcripts were identified providing a valuable resource for the identification of the biosynthetic mechanism responsible for the production of carnosic acid-family metabolites in S. pomifera. CONCLUSIONS: Our work has uncovered the key enzymes involved in miltiradiene biosynthesis in S. pomifera leaf glandular trichomes. The transcriptomic dataset obtained provides a valuable tool for the identification of the CYPs involved in the synthesis of carnosic acid-family metabolites. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-015-2147-3) contains supplementary material, which is available to authorized users. BioMed Central 2015-11-14 /pmc/articles/PMC4647624/ /pubmed/26572682 http://dx.doi.org/10.1186/s12864-015-2147-3 Text en © Trikka et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Trikka, Fotini A.
Nikolaidis, Alexandros
Ignea, Codruta
Tsaballa, Aphrodite
Tziveleka, Leto-Aikaterini
Ioannou, Efstathia
Roussis, Vassilios
Stea, Eleni A.
Božić, Dragana
Argiriou, Anagnostis
Kanellis, Angelos K.
Kampranis, Sotirios C.
Makris, Antonios M.
Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes
title Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes
title_full Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes
title_fullStr Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes
title_full_unstemmed Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes
title_short Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes
title_sort combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in s. pomifera glandular trichomes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647624/
https://www.ncbi.nlm.nih.gov/pubmed/26572682
http://dx.doi.org/10.1186/s12864-015-2147-3
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