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Impact of p16 status on pro- and anti-angiogenesis factors in head and neck cancers
BACKGROUND: Head and neck cancers (HNC) are aggressive tumours. Overexpression of p16 in HNC correlates with human papilloma virus (HPV)-associated HNC that carry a better prognosis than HPV-negative tumours. Angiogenesis is an important factor in tumour progression. Our aim was to dissect the impac...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647678/ https://www.ncbi.nlm.nih.gov/pubmed/26171937 http://dx.doi.org/10.1038/bjc.2015.251 |
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author | Baruah, P Lee, M Wilson, P O G Odutoye, T Williamson, P Hyde, N Kaski, J C Dumitriu, I E |
author_facet | Baruah, P Lee, M Wilson, P O G Odutoye, T Williamson, P Hyde, N Kaski, J C Dumitriu, I E |
author_sort | Baruah, P |
collection | PubMed |
description | BACKGROUND: Head and neck cancers (HNC) are aggressive tumours. Overexpression of p16 in HNC correlates with human papilloma virus (HPV)-associated HNC that carry a better prognosis than HPV-negative tumours. Angiogenesis is an important factor in tumour progression. Our aim was to dissect the impact of p16 expression on angiogenesis factors in HNC. METHODS: Eighteen newly diagnosed HNC patients and controls were analysed. Eleven pro- and anti-angiogenesis factors were quantified using multiplex ELISA in HNC patients and controls. Angiogenesis factors were analysed in tumour tissue using immunohistochemistry. RESULTS: Circulating levels of endostatin (anti-angiogenesis factor) were higher in the HNC group compared with healthy donors. Interestingly, the pro-angiogenesis factors angiopoietin-1 and vascular endothelial growth factor (VEGF) were significantly higher in patients with p16-negative compared with p16-positive HNC. Moreover, the major source of VEGF in p16-positive HNC tissue was tumour stromal cells. In contrast, both tumour cells and stromal cells expressed VEGF in p16-negative tissue. CONCLUSIONS: We show that p16-negative tumours associate with increased circulating levels of pro-angiogenic VEGF and angiopoietin-1. Tissue expression of VEGF differs between p16-positive and p16-negative tumours. These findings may explain differences in the biological behaviour of p16-positive and p16-negative HNC. Better understanding of mechanisms by which the p16 status influences tumour angiogenesis may guide the development of targeted therapies. |
format | Online Article Text |
id | pubmed-4647678 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-46476782016-08-11 Impact of p16 status on pro- and anti-angiogenesis factors in head and neck cancers Baruah, P Lee, M Wilson, P O G Odutoye, T Williamson, P Hyde, N Kaski, J C Dumitriu, I E Br J Cancer Molecular Diagnostics BACKGROUND: Head and neck cancers (HNC) are aggressive tumours. Overexpression of p16 in HNC correlates with human papilloma virus (HPV)-associated HNC that carry a better prognosis than HPV-negative tumours. Angiogenesis is an important factor in tumour progression. Our aim was to dissect the impact of p16 expression on angiogenesis factors in HNC. METHODS: Eighteen newly diagnosed HNC patients and controls were analysed. Eleven pro- and anti-angiogenesis factors were quantified using multiplex ELISA in HNC patients and controls. Angiogenesis factors were analysed in tumour tissue using immunohistochemistry. RESULTS: Circulating levels of endostatin (anti-angiogenesis factor) were higher in the HNC group compared with healthy donors. Interestingly, the pro-angiogenesis factors angiopoietin-1 and vascular endothelial growth factor (VEGF) were significantly higher in patients with p16-negative compared with p16-positive HNC. Moreover, the major source of VEGF in p16-positive HNC tissue was tumour stromal cells. In contrast, both tumour cells and stromal cells expressed VEGF in p16-negative tissue. CONCLUSIONS: We show that p16-negative tumours associate with increased circulating levels of pro-angiogenic VEGF and angiopoietin-1. Tissue expression of VEGF differs between p16-positive and p16-negative tumours. These findings may explain differences in the biological behaviour of p16-positive and p16-negative HNC. Better understanding of mechanisms by which the p16 status influences tumour angiogenesis may guide the development of targeted therapies. Nature Publishing Group 2015-08-11 2015-07-14 /pmc/articles/PMC4647678/ /pubmed/26171937 http://dx.doi.org/10.1038/bjc.2015.251 Text en Copyright © 2015 Cancer Research UK http://creativecommons.org/licenses/by-nc-sa/4.0/ From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/ |
spellingShingle | Molecular Diagnostics Baruah, P Lee, M Wilson, P O G Odutoye, T Williamson, P Hyde, N Kaski, J C Dumitriu, I E Impact of p16 status on pro- and anti-angiogenesis factors in head and neck cancers |
title | Impact of p16 status on pro- and anti-angiogenesis factors in head and neck cancers |
title_full | Impact of p16 status on pro- and anti-angiogenesis factors in head and neck cancers |
title_fullStr | Impact of p16 status on pro- and anti-angiogenesis factors in head and neck cancers |
title_full_unstemmed | Impact of p16 status on pro- and anti-angiogenesis factors in head and neck cancers |
title_short | Impact of p16 status on pro- and anti-angiogenesis factors in head and neck cancers |
title_sort | impact of p16 status on pro- and anti-angiogenesis factors in head and neck cancers |
topic | Molecular Diagnostics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4647678/ https://www.ncbi.nlm.nih.gov/pubmed/26171937 http://dx.doi.org/10.1038/bjc.2015.251 |
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