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Cervical intraepithelial neoplasia disease progression is associated with increased vaginal microbiome diversity

Persistent infection with oncogenic Human Papillomavirus (HPV) is necessary for cervical carcinogenesis. Although evidence suggests that the vaginal microbiome plays a functional role in the persistence or regression of HPV infections, this has yet to be described in women with cervical intra-epithe...

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Autores principales: Mitra, A., MacIntyre, D. A., Lee, Y. S., Smith, A., Marchesi, J. R., Lehne, B., Bhatia, R., Lyons, D., Paraskevaidis, E., Li, J. V., Holmes, E., Nicholson, J. K., Bennett, P. R., Kyrgiou, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4648063/
https://www.ncbi.nlm.nih.gov/pubmed/26574055
http://dx.doi.org/10.1038/srep16865
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author Mitra, A.
MacIntyre, D. A.
Lee, Y. S.
Smith, A.
Marchesi, J. R.
Lehne, B.
Bhatia, R.
Lyons, D.
Paraskevaidis, E.
Li, J. V.
Holmes, E.
Nicholson, J. K.
Bennett, P. R.
Kyrgiou, M.
author_facet Mitra, A.
MacIntyre, D. A.
Lee, Y. S.
Smith, A.
Marchesi, J. R.
Lehne, B.
Bhatia, R.
Lyons, D.
Paraskevaidis, E.
Li, J. V.
Holmes, E.
Nicholson, J. K.
Bennett, P. R.
Kyrgiou, M.
author_sort Mitra, A.
collection PubMed
description Persistent infection with oncogenic Human Papillomavirus (HPV) is necessary for cervical carcinogenesis. Although evidence suggests that the vaginal microbiome plays a functional role in the persistence or regression of HPV infections, this has yet to be described in women with cervical intra-epithelial neoplasia (CIN). We hypothesised that increasing microbiome diversity is associated with increasing CIN severity. llumina MiSeq sequencing of 16S rRNA gene amplicons was used to characterise the vaginal microbiota of women with low-grade squamous intra-epithelial lesions (LSIL; n = 52), high-grade (HSIL; n = 92), invasive cervical cancer (ICC; n = 5) and healthy controls (n = 20). Hierarchical clustering analysis revealed an increased prevalence of microbiomes characterised by high-diversity and low levels of Lactobacillus spp. (community state type-CST IV) with increasing disease severity, irrespective of HPV status (Normal = 2/20,10%; LSIL = 11/52,21%; HSIL = 25/92,27%; ICC = 2/5,40%). Increasing disease severity was associated with decreasing relative abundance of Lactobacillus spp. The vaginal microbiome in HSIL was characterised by higher levels of Sneathia sanguinegens (P < 0.01), Anaerococcus tetradius (P < 0.05) and Peptostreptococcus anaerobius (P < 0.05) and lower levels of Lactobacillus jensenii (P < 0.01) compared to LSIL. Our results suggest advancing CIN disease severity is associated with increasing vaginal microbiota diversity and may be involved in regulating viral persistence and disease progression.
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spelling pubmed-46480632015-11-23 Cervical intraepithelial neoplasia disease progression is associated with increased vaginal microbiome diversity Mitra, A. MacIntyre, D. A. Lee, Y. S. Smith, A. Marchesi, J. R. Lehne, B. Bhatia, R. Lyons, D. Paraskevaidis, E. Li, J. V. Holmes, E. Nicholson, J. K. Bennett, P. R. Kyrgiou, M. Sci Rep Article Persistent infection with oncogenic Human Papillomavirus (HPV) is necessary for cervical carcinogenesis. Although evidence suggests that the vaginal microbiome plays a functional role in the persistence or regression of HPV infections, this has yet to be described in women with cervical intra-epithelial neoplasia (CIN). We hypothesised that increasing microbiome diversity is associated with increasing CIN severity. llumina MiSeq sequencing of 16S rRNA gene amplicons was used to characterise the vaginal microbiota of women with low-grade squamous intra-epithelial lesions (LSIL; n = 52), high-grade (HSIL; n = 92), invasive cervical cancer (ICC; n = 5) and healthy controls (n = 20). Hierarchical clustering analysis revealed an increased prevalence of microbiomes characterised by high-diversity and low levels of Lactobacillus spp. (community state type-CST IV) with increasing disease severity, irrespective of HPV status (Normal = 2/20,10%; LSIL = 11/52,21%; HSIL = 25/92,27%; ICC = 2/5,40%). Increasing disease severity was associated with decreasing relative abundance of Lactobacillus spp. The vaginal microbiome in HSIL was characterised by higher levels of Sneathia sanguinegens (P < 0.01), Anaerococcus tetradius (P < 0.05) and Peptostreptococcus anaerobius (P < 0.05) and lower levels of Lactobacillus jensenii (P < 0.01) compared to LSIL. Our results suggest advancing CIN disease severity is associated with increasing vaginal microbiota diversity and may be involved in regulating viral persistence and disease progression. Nature Publishing Group 2015-11-17 /pmc/articles/PMC4648063/ /pubmed/26574055 http://dx.doi.org/10.1038/srep16865 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Mitra, A.
MacIntyre, D. A.
Lee, Y. S.
Smith, A.
Marchesi, J. R.
Lehne, B.
Bhatia, R.
Lyons, D.
Paraskevaidis, E.
Li, J. V.
Holmes, E.
Nicholson, J. K.
Bennett, P. R.
Kyrgiou, M.
Cervical intraepithelial neoplasia disease progression is associated with increased vaginal microbiome diversity
title Cervical intraepithelial neoplasia disease progression is associated with increased vaginal microbiome diversity
title_full Cervical intraepithelial neoplasia disease progression is associated with increased vaginal microbiome diversity
title_fullStr Cervical intraepithelial neoplasia disease progression is associated with increased vaginal microbiome diversity
title_full_unstemmed Cervical intraepithelial neoplasia disease progression is associated with increased vaginal microbiome diversity
title_short Cervical intraepithelial neoplasia disease progression is associated with increased vaginal microbiome diversity
title_sort cervical intraepithelial neoplasia disease progression is associated with increased vaginal microbiome diversity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4648063/
https://www.ncbi.nlm.nih.gov/pubmed/26574055
http://dx.doi.org/10.1038/srep16865
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