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Impaired formation of homotypic cell-in-cell structures in human tumor cells lacking alpha-catenin expression

Although cell-in-cell structures (CICs) could be detected in a wide range of human tumors, homotypic CICs formed between tumor cells occur at low rate for most of them. We recently reported that tumor cells lacking expression of E- and P-cadherin were incapable of forming homotypic CICs by entosis,...

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Autores principales: Wang, Manna, Ning, Xiangkai, Chen, Ang, Huang, Hongyan, Ni, Chao, Zhou, Changxi, Yu, Kaitao, Lan, Sanchun, Wang, Qiwei, Li, Shichong, Liu, Hong, Wang, Xiaoning, Chen, Zhaolie, Ma, Li, Sun, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4648412/
https://www.ncbi.nlm.nih.gov/pubmed/26192076
http://dx.doi.org/10.1038/srep12223
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author Wang, Manna
Ning, Xiangkai
Chen, Ang
Huang, Hongyan
Ni, Chao
Zhou, Changxi
Yu, Kaitao
Lan, Sanchun
Wang, Qiwei
Li, Shichong
Liu, Hong
Wang, Xiaoning
Chen, Zhaolie
Ma, Li
Sun, Qiang
author_facet Wang, Manna
Ning, Xiangkai
Chen, Ang
Huang, Hongyan
Ni, Chao
Zhou, Changxi
Yu, Kaitao
Lan, Sanchun
Wang, Qiwei
Li, Shichong
Liu, Hong
Wang, Xiaoning
Chen, Zhaolie
Ma, Li
Sun, Qiang
author_sort Wang, Manna
collection PubMed
description Although cell-in-cell structures (CICs) could be detected in a wide range of human tumors, homotypic CICs formed between tumor cells occur at low rate for most of them. We recently reported that tumor cells lacking expression of E- and P-cadherin were incapable of forming homotypic CICs by entosis, and re-expression of E- or P-cadherin was sufficient to induce CICs formation in these tumor cells. In this work, we found that homotypic CICs formation was impaired in some tumor cells expressing high level of E-cadherin due to loss expression of alpha-catenin (α-catenin), a molecular linker between cadherin-mediated adherens junctions and F-actin. Expression of α-catenin in these tumor cells restored cell-cell adhesion and promoted CICs formation in a ROCK kinase-dependent way. Thus, our work identified α-catenin as another molecule in addition to E- and P-cadherin that were targeted to inactivate homotypic CICs formation in human tumor cells.
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spelling pubmed-46484122015-11-23 Impaired formation of homotypic cell-in-cell structures in human tumor cells lacking alpha-catenin expression Wang, Manna Ning, Xiangkai Chen, Ang Huang, Hongyan Ni, Chao Zhou, Changxi Yu, Kaitao Lan, Sanchun Wang, Qiwei Li, Shichong Liu, Hong Wang, Xiaoning Chen, Zhaolie Ma, Li Sun, Qiang Sci Rep Article Although cell-in-cell structures (CICs) could be detected in a wide range of human tumors, homotypic CICs formed between tumor cells occur at low rate for most of them. We recently reported that tumor cells lacking expression of E- and P-cadherin were incapable of forming homotypic CICs by entosis, and re-expression of E- or P-cadherin was sufficient to induce CICs formation in these tumor cells. In this work, we found that homotypic CICs formation was impaired in some tumor cells expressing high level of E-cadherin due to loss expression of alpha-catenin (α-catenin), a molecular linker between cadherin-mediated adherens junctions and F-actin. Expression of α-catenin in these tumor cells restored cell-cell adhesion and promoted CICs formation in a ROCK kinase-dependent way. Thus, our work identified α-catenin as another molecule in addition to E- and P-cadherin that were targeted to inactivate homotypic CICs formation in human tumor cells. Nature Publishing Group 2015-07-20 /pmc/articles/PMC4648412/ /pubmed/26192076 http://dx.doi.org/10.1038/srep12223 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Wang, Manna
Ning, Xiangkai
Chen, Ang
Huang, Hongyan
Ni, Chao
Zhou, Changxi
Yu, Kaitao
Lan, Sanchun
Wang, Qiwei
Li, Shichong
Liu, Hong
Wang, Xiaoning
Chen, Zhaolie
Ma, Li
Sun, Qiang
Impaired formation of homotypic cell-in-cell structures in human tumor cells lacking alpha-catenin expression
title Impaired formation of homotypic cell-in-cell structures in human tumor cells lacking alpha-catenin expression
title_full Impaired formation of homotypic cell-in-cell structures in human tumor cells lacking alpha-catenin expression
title_fullStr Impaired formation of homotypic cell-in-cell structures in human tumor cells lacking alpha-catenin expression
title_full_unstemmed Impaired formation of homotypic cell-in-cell structures in human tumor cells lacking alpha-catenin expression
title_short Impaired formation of homotypic cell-in-cell structures in human tumor cells lacking alpha-catenin expression
title_sort impaired formation of homotypic cell-in-cell structures in human tumor cells lacking alpha-catenin expression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4648412/
https://www.ncbi.nlm.nih.gov/pubmed/26192076
http://dx.doi.org/10.1038/srep12223
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