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Association of astrocytes with neurons and astrocytes derived from distinct progenitor domains in the subpallium
Astrocytes play pivotal roles in metabolism and homeostasis as well as in neural development and function in a manner thought to depend on their region-specific diversity. In the mouse spinal cord, astrocytes and neurons, which are derived from a common progenitor domain (PD) and controlled by commo...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4648416/ https://www.ncbi.nlm.nih.gov/pubmed/26193445 http://dx.doi.org/10.1038/srep12258 |
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author | Torigoe, Makio Yamauchi, Kenta Zhu, Yan Kobayashi, Hiroaki Murakami, Fujio |
author_facet | Torigoe, Makio Yamauchi, Kenta Zhu, Yan Kobayashi, Hiroaki Murakami, Fujio |
author_sort | Torigoe, Makio |
collection | PubMed |
description | Astrocytes play pivotal roles in metabolism and homeostasis as well as in neural development and function in a manner thought to depend on their region-specific diversity. In the mouse spinal cord, astrocytes and neurons, which are derived from a common progenitor domain (PD) and controlled by common PD-specific transcription factors, migrate radially and share their final positions. However, whether astrocytes can only interact with neurons from common PDs in the brain remains unknown. Here, we focused on subpallium-derived cells, because the subpallium generates neurons that show a diverse mode of migration. We tracked their fate by in utero electroporation of plasmids that allow for chromosomal integration of transgenes or of a Cre recombinase expression vector to reporter mice. We also used an Nkx2.1(Cre) mouse line to fate map the cells originating from the medial ganglionic eminence and preoptic area. We find that although neurons and astrocytes are labeled in various regions, only neurons are labeled in the neocortex, hippocampus and olfactory bulb. Furthermore, we find astrocytes derived from an Nkx 2.1-negative PD are associated with neurons from the Nkx2.1(+) PD. Thus, forebrain astrocytes can associate with neurons as well as astrocytes derived from a distinct PD. |
format | Online Article Text |
id | pubmed-4648416 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-46484162015-11-23 Association of astrocytes with neurons and astrocytes derived from distinct progenitor domains in the subpallium Torigoe, Makio Yamauchi, Kenta Zhu, Yan Kobayashi, Hiroaki Murakami, Fujio Sci Rep Article Astrocytes play pivotal roles in metabolism and homeostasis as well as in neural development and function in a manner thought to depend on their region-specific diversity. In the mouse spinal cord, astrocytes and neurons, which are derived from a common progenitor domain (PD) and controlled by common PD-specific transcription factors, migrate radially and share their final positions. However, whether astrocytes can only interact with neurons from common PDs in the brain remains unknown. Here, we focused on subpallium-derived cells, because the subpallium generates neurons that show a diverse mode of migration. We tracked their fate by in utero electroporation of plasmids that allow for chromosomal integration of transgenes or of a Cre recombinase expression vector to reporter mice. We also used an Nkx2.1(Cre) mouse line to fate map the cells originating from the medial ganglionic eminence and preoptic area. We find that although neurons and astrocytes are labeled in various regions, only neurons are labeled in the neocortex, hippocampus and olfactory bulb. Furthermore, we find astrocytes derived from an Nkx 2.1-negative PD are associated with neurons from the Nkx2.1(+) PD. Thus, forebrain astrocytes can associate with neurons as well as astrocytes derived from a distinct PD. Nature Publishing Group 2015-07-20 /pmc/articles/PMC4648416/ /pubmed/26193445 http://dx.doi.org/10.1038/srep12258 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Torigoe, Makio Yamauchi, Kenta Zhu, Yan Kobayashi, Hiroaki Murakami, Fujio Association of astrocytes with neurons and astrocytes derived from distinct progenitor domains in the subpallium |
title | Association of astrocytes with neurons and astrocytes derived from distinct progenitor domains in the subpallium |
title_full | Association of astrocytes with neurons and astrocytes derived from distinct progenitor domains in the subpallium |
title_fullStr | Association of astrocytes with neurons and astrocytes derived from distinct progenitor domains in the subpallium |
title_full_unstemmed | Association of astrocytes with neurons and astrocytes derived from distinct progenitor domains in the subpallium |
title_short | Association of astrocytes with neurons and astrocytes derived from distinct progenitor domains in the subpallium |
title_sort | association of astrocytes with neurons and astrocytes derived from distinct progenitor domains in the subpallium |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4648416/ https://www.ncbi.nlm.nih.gov/pubmed/26193445 http://dx.doi.org/10.1038/srep12258 |
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