Cargando…

Prevalence of Plasmid-Mediated Quinolone Resistance Genes among Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Human Isolates in Iran

The purpose of this study was to determine the prevalence and molecular characterization of plasmid-mediated quinolone resistance (PMQR) genes (qnrA, qnrB, qnrS, aac(6′)-Ib-cr, and qepA) among ESBL-producing Klebsiella pneumoniae isolates in Kashan, Iran. A total of 185 K. pneumoniae isolates were t...

Descripción completa

Detalles Bibliográficos
Autores principales: Shams, Ehsaneh, Firoozeh, Farzaneh, Moniri, Rezvan, Zibaei, Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4649097/
https://www.ncbi.nlm.nih.gov/pubmed/26618005
http://dx.doi.org/10.1155/2015/434391
_version_ 1782401312642564096
author Shams, Ehsaneh
Firoozeh, Farzaneh
Moniri, Rezvan
Zibaei, Mohammad
author_facet Shams, Ehsaneh
Firoozeh, Farzaneh
Moniri, Rezvan
Zibaei, Mohammad
author_sort Shams, Ehsaneh
collection PubMed
description The purpose of this study was to determine the prevalence and molecular characterization of plasmid-mediated quinolone resistance (PMQR) genes (qnrA, qnrB, qnrS, aac(6′)-Ib-cr, and qepA) among ESBL-producing Klebsiella pneumoniae isolates in Kashan, Iran. A total of 185 K. pneumoniae isolates were tested for quinolone resistance and ESBL-producing using the disk diffusion method and double disk synergy (DDST) confirmatory test. ESBL-producing strains were further evaluated for the bla (CTX-M) genes. The PCR method was used to show presence of plasmid-mediated quinolone resistance genes and the purified PCR products were sequenced. Eighty-seven ESBL-producing strains were identified by DDST confirmatory test and majority (70, 80.5%) of which carried bla (CTX-M) genes including CTX-M-1 (60%), CTX-M-2 (42.9%), and CTX-M-9 (34.3%). Seventy-seven ESBL-producing K. pneumoniae isolates harbored PMQR genes, which mostly consisted of aac(6′)-Ib-cr (70.1%) and qnrB (46.0%), followed by qnrS (5.7%). Among the 77 PMQR-positive isolates, 27 (35.1%) and 1 (1.3%) carried 2 and 3 different PMQR genes, respectively. However, qnrA and qepA were not found in any isolate. Our results highlight high ESBL occurrence with CTX-M type and high frequency of plasmid-mediated quinolone resistance genes among ESBL-producing K. pneumoniae isolates in Kashan.
format Online
Article
Text
id pubmed-4649097
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-46490972015-11-29 Prevalence of Plasmid-Mediated Quinolone Resistance Genes among Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Human Isolates in Iran Shams, Ehsaneh Firoozeh, Farzaneh Moniri, Rezvan Zibaei, Mohammad J Pathog Research Article The purpose of this study was to determine the prevalence and molecular characterization of plasmid-mediated quinolone resistance (PMQR) genes (qnrA, qnrB, qnrS, aac(6′)-Ib-cr, and qepA) among ESBL-producing Klebsiella pneumoniae isolates in Kashan, Iran. A total of 185 K. pneumoniae isolates were tested for quinolone resistance and ESBL-producing using the disk diffusion method and double disk synergy (DDST) confirmatory test. ESBL-producing strains were further evaluated for the bla (CTX-M) genes. The PCR method was used to show presence of plasmid-mediated quinolone resistance genes and the purified PCR products were sequenced. Eighty-seven ESBL-producing strains were identified by DDST confirmatory test and majority (70, 80.5%) of which carried bla (CTX-M) genes including CTX-M-1 (60%), CTX-M-2 (42.9%), and CTX-M-9 (34.3%). Seventy-seven ESBL-producing K. pneumoniae isolates harbored PMQR genes, which mostly consisted of aac(6′)-Ib-cr (70.1%) and qnrB (46.0%), followed by qnrS (5.7%). Among the 77 PMQR-positive isolates, 27 (35.1%) and 1 (1.3%) carried 2 and 3 different PMQR genes, respectively. However, qnrA and qepA were not found in any isolate. Our results highlight high ESBL occurrence with CTX-M type and high frequency of plasmid-mediated quinolone resistance genes among ESBL-producing K. pneumoniae isolates in Kashan. Hindawi Publishing Corporation 2015 2015-11-04 /pmc/articles/PMC4649097/ /pubmed/26618005 http://dx.doi.org/10.1155/2015/434391 Text en Copyright © 2015 Ehsaneh Shams et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Shams, Ehsaneh
Firoozeh, Farzaneh
Moniri, Rezvan
Zibaei, Mohammad
Prevalence of Plasmid-Mediated Quinolone Resistance Genes among Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Human Isolates in Iran
title Prevalence of Plasmid-Mediated Quinolone Resistance Genes among Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Human Isolates in Iran
title_full Prevalence of Plasmid-Mediated Quinolone Resistance Genes among Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Human Isolates in Iran
title_fullStr Prevalence of Plasmid-Mediated Quinolone Resistance Genes among Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Human Isolates in Iran
title_full_unstemmed Prevalence of Plasmid-Mediated Quinolone Resistance Genes among Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Human Isolates in Iran
title_short Prevalence of Plasmid-Mediated Quinolone Resistance Genes among Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Human Isolates in Iran
title_sort prevalence of plasmid-mediated quinolone resistance genes among extended-spectrum β-lactamase-producing klebsiella pneumoniae human isolates in iran
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4649097/
https://www.ncbi.nlm.nih.gov/pubmed/26618005
http://dx.doi.org/10.1155/2015/434391
work_keys_str_mv AT shamsehsaneh prevalenceofplasmidmediatedquinoloneresistancegenesamongextendedspectrumblactamaseproducingklebsiellapneumoniaehumanisolatesiniran
AT firoozehfarzaneh prevalenceofplasmidmediatedquinoloneresistancegenesamongextendedspectrumblactamaseproducingklebsiellapneumoniaehumanisolatesiniran
AT monirirezvan prevalenceofplasmidmediatedquinoloneresistancegenesamongextendedspectrumblactamaseproducingklebsiellapneumoniaehumanisolatesiniran
AT zibaeimohammad prevalenceofplasmidmediatedquinoloneresistancegenesamongextendedspectrumblactamaseproducingklebsiellapneumoniaehumanisolatesiniran