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The effect of arbutin on lipid peroxidation and antioxidant capacity in the serum of cyclosporine-treated rats

BACKGROUND: Cyclosporine A (CsA) is a potent immunosuppressant drug with therapeutic and toxic actions. The use of CsA is limited by its toxicity. Several researchers had proposed that oxidative stress could play an important role in CsA-induced toxicity. Arbutin has recently been shown to possess a...

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Autores principales: Khadir, Fatemeh, Pouramir, Mahdi, Joorsaraee, Seyyed Gholamali, Feizi, Farideh, Sorkhi, Hadi, Yousefi, Fatemeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Babol University of Medical Sciences 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4649267/
https://www.ncbi.nlm.nih.gov/pubmed/26644892
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author Khadir, Fatemeh
Pouramir, Mahdi
Joorsaraee, Seyyed Gholamali
Feizi, Farideh
Sorkhi, Hadi
Yousefi, Fatemeh
author_facet Khadir, Fatemeh
Pouramir, Mahdi
Joorsaraee, Seyyed Gholamali
Feizi, Farideh
Sorkhi, Hadi
Yousefi, Fatemeh
author_sort Khadir, Fatemeh
collection PubMed
description BACKGROUND: Cyclosporine A (CsA) is a potent immunosuppressant drug with therapeutic and toxic actions. The use of CsA is limited by its toxicity. Several researchers had proposed that oxidative stress could play an important role in CsA-induced toxicity. Arbutin has recently been shown to possess antioxidative and free radical scavenging abilities. The present study was designed to investigate the in vivo effects of arbutin on lipid peroxidation and antioxidant capacity in the serum of cyclosporine treated rats. METHODS: Adult male Wistar rats were divided into six groups (n=8/group): (I) control (no CsA and arbutin administration), (II and III) were treated subcutaneously (Sc) with arbutin (50,100 mg/kg/bw), respectively, (IV) administered CsA (25 mg/kg/bw) intraperitoneally (IP), (V and VI) received the combination of CsA (25 mg/kg/bw) i.p and arbutin (50,100 mg/kg/bw) Sc daily, respectively. At the end of the treatment (after3 weeks), serum lipid peroxidation was measured by thiobarbituric acid-reacting substances (TBARS) and serum total antioxidant capacity (ferric reducing ability of plasma [FRAP]) was assayed based on spectrophotometric method. RESULTS: TBARS had been significantly increased by CsA administration compared with control rats. Arbutin (50mg/kg/bw) completely prevented this effect, but arbutin (100 mg/kg/bw) alone or in combination with CsA significantly increased lipid peroxidation compared with controls. CONCLUSION: Our data indicate that arbutin (50mg/kg/bw) had protective effect in the CsA-induced toxicity but high concentration of arbutin (100mg/kg/bw) showed meaningful oxidative and lipoperoxidative effects.
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spelling pubmed-46492672015-12-07 The effect of arbutin on lipid peroxidation and antioxidant capacity in the serum of cyclosporine-treated rats Khadir, Fatemeh Pouramir, Mahdi Joorsaraee, Seyyed Gholamali Feizi, Farideh Sorkhi, Hadi Yousefi, Fatemeh Caspian J Intern Med Original Article BACKGROUND: Cyclosporine A (CsA) is a potent immunosuppressant drug with therapeutic and toxic actions. The use of CsA is limited by its toxicity. Several researchers had proposed that oxidative stress could play an important role in CsA-induced toxicity. Arbutin has recently been shown to possess antioxidative and free radical scavenging abilities. The present study was designed to investigate the in vivo effects of arbutin on lipid peroxidation and antioxidant capacity in the serum of cyclosporine treated rats. METHODS: Adult male Wistar rats were divided into six groups (n=8/group): (I) control (no CsA and arbutin administration), (II and III) were treated subcutaneously (Sc) with arbutin (50,100 mg/kg/bw), respectively, (IV) administered CsA (25 mg/kg/bw) intraperitoneally (IP), (V and VI) received the combination of CsA (25 mg/kg/bw) i.p and arbutin (50,100 mg/kg/bw) Sc daily, respectively. At the end of the treatment (after3 weeks), serum lipid peroxidation was measured by thiobarbituric acid-reacting substances (TBARS) and serum total antioxidant capacity (ferric reducing ability of plasma [FRAP]) was assayed based on spectrophotometric method. RESULTS: TBARS had been significantly increased by CsA administration compared with control rats. Arbutin (50mg/kg/bw) completely prevented this effect, but arbutin (100 mg/kg/bw) alone or in combination with CsA significantly increased lipid peroxidation compared with controls. CONCLUSION: Our data indicate that arbutin (50mg/kg/bw) had protective effect in the CsA-induced toxicity but high concentration of arbutin (100mg/kg/bw) showed meaningful oxidative and lipoperoxidative effects. Babol University of Medical Sciences 2015 /pmc/articles/PMC4649267/ /pubmed/26644892 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Khadir, Fatemeh
Pouramir, Mahdi
Joorsaraee, Seyyed Gholamali
Feizi, Farideh
Sorkhi, Hadi
Yousefi, Fatemeh
The effect of arbutin on lipid peroxidation and antioxidant capacity in the serum of cyclosporine-treated rats
title The effect of arbutin on lipid peroxidation and antioxidant capacity in the serum of cyclosporine-treated rats
title_full The effect of arbutin on lipid peroxidation and antioxidant capacity in the serum of cyclosporine-treated rats
title_fullStr The effect of arbutin on lipid peroxidation and antioxidant capacity in the serum of cyclosporine-treated rats
title_full_unstemmed The effect of arbutin on lipid peroxidation and antioxidant capacity in the serum of cyclosporine-treated rats
title_short The effect of arbutin on lipid peroxidation and antioxidant capacity in the serum of cyclosporine-treated rats
title_sort effect of arbutin on lipid peroxidation and antioxidant capacity in the serum of cyclosporine-treated rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4649267/
https://www.ncbi.nlm.nih.gov/pubmed/26644892
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