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Soft viscoelastic properties of nuclear actin age oocytes due to gravitational creep
The actin cytoskeleton helps maintain structural organization within living cells. In large X. laevis oocytes, gravity becomes a dominant force and is countered by a nuclear actin network that prevents liquid-like nuclear bodies from immediate sedimentation and coalescence. However, nuclear actin’s...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4649616/ https://www.ncbi.nlm.nih.gov/pubmed/26577186 http://dx.doi.org/10.1038/srep16607 |
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author | Feric, Marina Broedersz, Chase P. Brangwynne, Clifford P. |
author_facet | Feric, Marina Broedersz, Chase P. Brangwynne, Clifford P. |
author_sort | Feric, Marina |
collection | PubMed |
description | The actin cytoskeleton helps maintain structural organization within living cells. In large X. laevis oocytes, gravity becomes a dominant force and is countered by a nuclear actin network that prevents liquid-like nuclear bodies from immediate sedimentation and coalescence. However, nuclear actin’s mechanical properties, and how they facilitate the stabilization of nuclear bodies, remain unknown. Using active microrheology, we find that nuclear actin forms a weak viscoelastic network, with a modulus of roughly 0.1 Pa. Embedded probe particles subjected to a constant force exhibit continuous displacement, due to viscoelastic creep. Gravitational forces also cause creep displacement of nuclear bodies, resulting in their asymmetric nuclear distribution. Thus, nuclear actin does not indefinitely support the emulsion of nuclear bodies, but only kinetically stabilizes them by slowing down gravitational creep to ~2 months. This is similar to the viability time of large oocytes, suggesting gravitational creep ages oocytes, with fatal consequences on long timescales. |
format | Online Article Text |
id | pubmed-4649616 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-46496162015-11-23 Soft viscoelastic properties of nuclear actin age oocytes due to gravitational creep Feric, Marina Broedersz, Chase P. Brangwynne, Clifford P. Sci Rep Article The actin cytoskeleton helps maintain structural organization within living cells. In large X. laevis oocytes, gravity becomes a dominant force and is countered by a nuclear actin network that prevents liquid-like nuclear bodies from immediate sedimentation and coalescence. However, nuclear actin’s mechanical properties, and how they facilitate the stabilization of nuclear bodies, remain unknown. Using active microrheology, we find that nuclear actin forms a weak viscoelastic network, with a modulus of roughly 0.1 Pa. Embedded probe particles subjected to a constant force exhibit continuous displacement, due to viscoelastic creep. Gravitational forces also cause creep displacement of nuclear bodies, resulting in their asymmetric nuclear distribution. Thus, nuclear actin does not indefinitely support the emulsion of nuclear bodies, but only kinetically stabilizes them by slowing down gravitational creep to ~2 months. This is similar to the viability time of large oocytes, suggesting gravitational creep ages oocytes, with fatal consequences on long timescales. Nature Publishing Group 2015-11-18 /pmc/articles/PMC4649616/ /pubmed/26577186 http://dx.doi.org/10.1038/srep16607 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Feric, Marina Broedersz, Chase P. Brangwynne, Clifford P. Soft viscoelastic properties of nuclear actin age oocytes due to gravitational creep |
title | Soft viscoelastic properties of nuclear actin age oocytes due to gravitational creep |
title_full | Soft viscoelastic properties of nuclear actin age oocytes due to gravitational creep |
title_fullStr | Soft viscoelastic properties of nuclear actin age oocytes due to gravitational creep |
title_full_unstemmed | Soft viscoelastic properties of nuclear actin age oocytes due to gravitational creep |
title_short | Soft viscoelastic properties of nuclear actin age oocytes due to gravitational creep |
title_sort | soft viscoelastic properties of nuclear actin age oocytes due to gravitational creep |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4649616/ https://www.ncbi.nlm.nih.gov/pubmed/26577186 http://dx.doi.org/10.1038/srep16607 |
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