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Loss of CD147 results in impaired epithelial cell differentiation and malformation of the meibomian gland

Meibomian gland dysfunction is a leading cause of ocular surface disease. However, little is known about the regulatory processes that control the development and maintenance of this sebaceous gland. Here, we identify a novel function for CD147, a transmembrane protein that promotes tissue remodelin...

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Autores principales: Mauris, J, Dieckow, J, Schob, S, Pulli, B, Hatton, M P, Jeong, S, Bauskar, A, Gabison, E, Nowak, R, Argüeso, P
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4650560/
https://www.ncbi.nlm.nih.gov/pubmed/25880093
http://dx.doi.org/10.1038/cddis.2015.98
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author Mauris, J
Dieckow, J
Schob, S
Pulli, B
Hatton, M P
Jeong, S
Bauskar, A
Gabison, E
Nowak, R
Argüeso, P
author_facet Mauris, J
Dieckow, J
Schob, S
Pulli, B
Hatton, M P
Jeong, S
Bauskar, A
Gabison, E
Nowak, R
Argüeso, P
author_sort Mauris, J
collection PubMed
description Meibomian gland dysfunction is a leading cause of ocular surface disease. However, little is known about the regulatory processes that control the development and maintenance of this sebaceous gland. Here, we identify a novel function for CD147, a transmembrane protein that promotes tissue remodeling through induction of matrix metalloproteinases, in regulating meibocyte differentiation and activity. We found that CD147 localized along basal cells and within discrete membrane domains of differentiated meibocytes in glandular acini containing gelatinolytic activity. Induction of meibocyte differentiation in vitro promoted CD147 clustering and MMP9 secretion, whereas RNAi-mediated abrogation of CD147 impaired MMP9 secretion, concomitant with a reduction in the number of proliferative cells and cytoplasmic lipids. Meibomian glands of CD147 knockout mice had a lower number of acini in both the superior and inferior tarsal plates of the eyelids, and were characterized by loss of lipid-filled meibocytes compared with control mice. Together, our data provide evidence showing that gelatinolytic activity in meibocytes is dependent on CD147, and supports a role for CD147 in maintaining the normal development and function of the meibomian gland.
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spelling pubmed-46505602015-12-01 Loss of CD147 results in impaired epithelial cell differentiation and malformation of the meibomian gland Mauris, J Dieckow, J Schob, S Pulli, B Hatton, M P Jeong, S Bauskar, A Gabison, E Nowak, R Argüeso, P Cell Death Dis Original Article Meibomian gland dysfunction is a leading cause of ocular surface disease. However, little is known about the regulatory processes that control the development and maintenance of this sebaceous gland. Here, we identify a novel function for CD147, a transmembrane protein that promotes tissue remodeling through induction of matrix metalloproteinases, in regulating meibocyte differentiation and activity. We found that CD147 localized along basal cells and within discrete membrane domains of differentiated meibocytes in glandular acini containing gelatinolytic activity. Induction of meibocyte differentiation in vitro promoted CD147 clustering and MMP9 secretion, whereas RNAi-mediated abrogation of CD147 impaired MMP9 secretion, concomitant with a reduction in the number of proliferative cells and cytoplasmic lipids. Meibomian glands of CD147 knockout mice had a lower number of acini in both the superior and inferior tarsal plates of the eyelids, and were characterized by loss of lipid-filled meibocytes compared with control mice. Together, our data provide evidence showing that gelatinolytic activity in meibocytes is dependent on CD147, and supports a role for CD147 in maintaining the normal development and function of the meibomian gland. Nature Publishing Group 2015-04 2015-04-16 /pmc/articles/PMC4650560/ /pubmed/25880093 http://dx.doi.org/10.1038/cddis.2015.98 Text en Copyright © 2015 Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Original Article
Mauris, J
Dieckow, J
Schob, S
Pulli, B
Hatton, M P
Jeong, S
Bauskar, A
Gabison, E
Nowak, R
Argüeso, P
Loss of CD147 results in impaired epithelial cell differentiation and malformation of the meibomian gland
title Loss of CD147 results in impaired epithelial cell differentiation and malformation of the meibomian gland
title_full Loss of CD147 results in impaired epithelial cell differentiation and malformation of the meibomian gland
title_fullStr Loss of CD147 results in impaired epithelial cell differentiation and malformation of the meibomian gland
title_full_unstemmed Loss of CD147 results in impaired epithelial cell differentiation and malformation of the meibomian gland
title_short Loss of CD147 results in impaired epithelial cell differentiation and malformation of the meibomian gland
title_sort loss of cd147 results in impaired epithelial cell differentiation and malformation of the meibomian gland
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4650560/
https://www.ncbi.nlm.nih.gov/pubmed/25880093
http://dx.doi.org/10.1038/cddis.2015.98
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