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Dynamic tubulation of mitochondria drives mitochondrial network formation

Mitochondria form networks. Formation of mitochondrial networks is important for maintaining mitochondrial DNA integrity and interchanging mitochondrial material, whereas disruption of the mitochondrial network affects mitochondrial functions. According to the current view, mitochondrial networks ar...

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Autores principales: Wang, Chong, Du, Wanqing, Su, Qian Peter, Zhu, Mingli, Feng, Peiyuan, Li, Ying, Zhou, Yichen, Mi, Na, Zhu, Yueyao, Jiang, Dong, Zhang, Senyan, Zhang, Zerui, Sun, Yujie, Yu, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4650629/
https://www.ncbi.nlm.nih.gov/pubmed/26206315
http://dx.doi.org/10.1038/cr.2015.89
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author Wang, Chong
Du, Wanqing
Su, Qian Peter
Zhu, Mingli
Feng, Peiyuan
Li, Ying
Zhou, Yichen
Mi, Na
Zhu, Yueyao
Jiang, Dong
Zhang, Senyan
Zhang, Zerui
Sun, Yujie
Yu, Li
author_facet Wang, Chong
Du, Wanqing
Su, Qian Peter
Zhu, Mingli
Feng, Peiyuan
Li, Ying
Zhou, Yichen
Mi, Na
Zhu, Yueyao
Jiang, Dong
Zhang, Senyan
Zhang, Zerui
Sun, Yujie
Yu, Li
author_sort Wang, Chong
collection PubMed
description Mitochondria form networks. Formation of mitochondrial networks is important for maintaining mitochondrial DNA integrity and interchanging mitochondrial material, whereas disruption of the mitochondrial network affects mitochondrial functions. According to the current view, mitochondrial networks are formed by fusion of individual mitochondria. Here, we report a new mechanism for formation of mitochondrial networks through KIF5B-mediated dynamic tubulation of mitochondria. We found that KIF5B pulls thin, highly dynamic tubules out of mitochondria. Fusion of these dynamic tubules, which is mediated by mitofusins, gives rise to the mitochondrial network. We further demonstrated that dynamic tubulation and fusion is sufficient for mitochondrial network formation, by reconstituting mitochondrial networks in vitro using purified fusion-competent mitochondria, recombinant KIF5B, and polymerized microtubules. Interestingly, KIF5B only controls network formation in the peripheral zone of the cell, indicating that the mitochondrial network is divided into subzones, which may be constructed by different mechanisms. Our data not only uncover an essential mechanism for mitochondrial network formation, but also reveal that different parts of the mitochondrial network are formed by different mechanisms.
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spelling pubmed-46506292015-12-01 Dynamic tubulation of mitochondria drives mitochondrial network formation Wang, Chong Du, Wanqing Su, Qian Peter Zhu, Mingli Feng, Peiyuan Li, Ying Zhou, Yichen Mi, Na Zhu, Yueyao Jiang, Dong Zhang, Senyan Zhang, Zerui Sun, Yujie Yu, Li Cell Res Original Article Mitochondria form networks. Formation of mitochondrial networks is important for maintaining mitochondrial DNA integrity and interchanging mitochondrial material, whereas disruption of the mitochondrial network affects mitochondrial functions. According to the current view, mitochondrial networks are formed by fusion of individual mitochondria. Here, we report a new mechanism for formation of mitochondrial networks through KIF5B-mediated dynamic tubulation of mitochondria. We found that KIF5B pulls thin, highly dynamic tubules out of mitochondria. Fusion of these dynamic tubules, which is mediated by mitofusins, gives rise to the mitochondrial network. We further demonstrated that dynamic tubulation and fusion is sufficient for mitochondrial network formation, by reconstituting mitochondrial networks in vitro using purified fusion-competent mitochondria, recombinant KIF5B, and polymerized microtubules. Interestingly, KIF5B only controls network formation in the peripheral zone of the cell, indicating that the mitochondrial network is divided into subzones, which may be constructed by different mechanisms. Our data not only uncover an essential mechanism for mitochondrial network formation, but also reveal that different parts of the mitochondrial network are formed by different mechanisms. Nature Publishing Group 2015-10 2015-07-24 /pmc/articles/PMC4650629/ /pubmed/26206315 http://dx.doi.org/10.1038/cr.2015.89 Text en Copyright © 2015 Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences http://creativecommons.org/licenses/by-nc-nd/3.0 This work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0
spellingShingle Original Article
Wang, Chong
Du, Wanqing
Su, Qian Peter
Zhu, Mingli
Feng, Peiyuan
Li, Ying
Zhou, Yichen
Mi, Na
Zhu, Yueyao
Jiang, Dong
Zhang, Senyan
Zhang, Zerui
Sun, Yujie
Yu, Li
Dynamic tubulation of mitochondria drives mitochondrial network formation
title Dynamic tubulation of mitochondria drives mitochondrial network formation
title_full Dynamic tubulation of mitochondria drives mitochondrial network formation
title_fullStr Dynamic tubulation of mitochondria drives mitochondrial network formation
title_full_unstemmed Dynamic tubulation of mitochondria drives mitochondrial network formation
title_short Dynamic tubulation of mitochondria drives mitochondrial network formation
title_sort dynamic tubulation of mitochondria drives mitochondrial network formation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4650629/
https://www.ncbi.nlm.nih.gov/pubmed/26206315
http://dx.doi.org/10.1038/cr.2015.89
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