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Bicarbonate transporters in corals point towards a key step in the evolution of cnidarian calcification

The bicarbonate ion (HCO(3)(−)) is involved in two major physiological processes in corals, biomineralization and photosynthesis, yet no molecular data on bicarbonate transporters are available. Here, we characterized plasma membrane-type HCO(3)(−) transporters in the scleractinian coral Stylophora...

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Autores principales: Zoccola, Didier, Ganot, Philippe, Bertucci, Anthony, Caminiti-Segonds, Natacha, Techer, Nathalie, Voolstra, Christian R, Aranda, Manuel, Tambutté, Eric, Allemand, Denis, Casey, Joseph R, Tambutté, Sylvie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4650655/
https://www.ncbi.nlm.nih.gov/pubmed/26040894
http://dx.doi.org/10.1038/srep09983
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author Zoccola, Didier
Ganot, Philippe
Bertucci, Anthony
Caminiti-Segonds, Natacha
Techer, Nathalie
Voolstra, Christian R
Aranda, Manuel
Tambutté, Eric
Allemand, Denis
Casey, Joseph R
Tambutté, Sylvie
author_facet Zoccola, Didier
Ganot, Philippe
Bertucci, Anthony
Caminiti-Segonds, Natacha
Techer, Nathalie
Voolstra, Christian R
Aranda, Manuel
Tambutté, Eric
Allemand, Denis
Casey, Joseph R
Tambutté, Sylvie
author_sort Zoccola, Didier
collection PubMed
description The bicarbonate ion (HCO(3)(−)) is involved in two major physiological processes in corals, biomineralization and photosynthesis, yet no molecular data on bicarbonate transporters are available. Here, we characterized plasma membrane-type HCO(3)(−) transporters in the scleractinian coral Stylophora pistillata. Eight solute carrier (SLC) genes were found in the genome: five homologs of mammalian-type SLC4 family members, and three of mammalian-type SLC26 family members. Using relative expression analysis and immunostaining, we analyzed the cellular distribution of these transporters and conducted phylogenetic analyses to determine the extent of conservation among cnidarian model organisms. Our data suggest that the SLC4γ isoform is specific to scleractinian corals and responsible for supplying HCO(3)(−) to the site of calcification. Taken together, SLC4γ appears to be one of the key genes for skeleton building in corals, which bears profound implications for our understanding of coral biomineralization and the evolution of scleractinian corals within cnidarians.
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spelling pubmed-46506552015-11-24 Bicarbonate transporters in corals point towards a key step in the evolution of cnidarian calcification Zoccola, Didier Ganot, Philippe Bertucci, Anthony Caminiti-Segonds, Natacha Techer, Nathalie Voolstra, Christian R Aranda, Manuel Tambutté, Eric Allemand, Denis Casey, Joseph R Tambutté, Sylvie Sci Rep Article The bicarbonate ion (HCO(3)(−)) is involved in two major physiological processes in corals, biomineralization and photosynthesis, yet no molecular data on bicarbonate transporters are available. Here, we characterized plasma membrane-type HCO(3)(−) transporters in the scleractinian coral Stylophora pistillata. Eight solute carrier (SLC) genes were found in the genome: five homologs of mammalian-type SLC4 family members, and three of mammalian-type SLC26 family members. Using relative expression analysis and immunostaining, we analyzed the cellular distribution of these transporters and conducted phylogenetic analyses to determine the extent of conservation among cnidarian model organisms. Our data suggest that the SLC4γ isoform is specific to scleractinian corals and responsible for supplying HCO(3)(−) to the site of calcification. Taken together, SLC4γ appears to be one of the key genes for skeleton building in corals, which bears profound implications for our understanding of coral biomineralization and the evolution of scleractinian corals within cnidarians. Nature Publishing Group 2015-06-04 /pmc/articles/PMC4650655/ /pubmed/26040894 http://dx.doi.org/10.1038/srep09983 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Zoccola, Didier
Ganot, Philippe
Bertucci, Anthony
Caminiti-Segonds, Natacha
Techer, Nathalie
Voolstra, Christian R
Aranda, Manuel
Tambutté, Eric
Allemand, Denis
Casey, Joseph R
Tambutté, Sylvie
Bicarbonate transporters in corals point towards a key step in the evolution of cnidarian calcification
title Bicarbonate transporters in corals point towards a key step in the evolution of cnidarian calcification
title_full Bicarbonate transporters in corals point towards a key step in the evolution of cnidarian calcification
title_fullStr Bicarbonate transporters in corals point towards a key step in the evolution of cnidarian calcification
title_full_unstemmed Bicarbonate transporters in corals point towards a key step in the evolution of cnidarian calcification
title_short Bicarbonate transporters in corals point towards a key step in the evolution of cnidarian calcification
title_sort bicarbonate transporters in corals point towards a key step in the evolution of cnidarian calcification
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4650655/
https://www.ncbi.nlm.nih.gov/pubmed/26040894
http://dx.doi.org/10.1038/srep09983
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