Cargando…

Identification of a distinct population of CD133(+)CXCR4(+) cancer stem cells in ovarian cancer

CD133 and CXCR4 were evaluated in the NCI-60 cell lines to identify cancer stem cell rich populations. Screening revealed that, ovarian OVCAR-3, -4 and -5 and colon cancer HT-29, HCT-116 and SW620 over expressed both proteins. We aimed to isolate cells with stem cell features sorting the cells expre...

Descripción completa

Detalles Bibliográficos
Autores principales: Cioffi, Michele, D’Alterio, Crescenzo, Camerlingo, Rosalba, Tirino, Virginia, Consales, Claudia, Riccio, Anna, Ieranò, Caterina, Cecere, Sabrina Chiara, Losito, Nunzia Simona, Greggi, Stefano, Pignata, Sandro, Pirozzi, Giuseppe, Scala, Stefania
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4650662/
https://www.ncbi.nlm.nih.gov/pubmed/26020117
http://dx.doi.org/10.1038/srep10357
_version_ 1782401532001517568
author Cioffi, Michele
D’Alterio, Crescenzo
Camerlingo, Rosalba
Tirino, Virginia
Consales, Claudia
Riccio, Anna
Ieranò, Caterina
Cecere, Sabrina Chiara
Losito, Nunzia Simona
Greggi, Stefano
Pignata, Sandro
Pirozzi, Giuseppe
Scala, Stefania
author_facet Cioffi, Michele
D’Alterio, Crescenzo
Camerlingo, Rosalba
Tirino, Virginia
Consales, Claudia
Riccio, Anna
Ieranò, Caterina
Cecere, Sabrina Chiara
Losito, Nunzia Simona
Greggi, Stefano
Pignata, Sandro
Pirozzi, Giuseppe
Scala, Stefania
author_sort Cioffi, Michele
collection PubMed
description CD133 and CXCR4 were evaluated in the NCI-60 cell lines to identify cancer stem cell rich populations. Screening revealed that, ovarian OVCAR-3, -4 and -5 and colon cancer HT-29, HCT-116 and SW620 over expressed both proteins. We aimed to isolate cells with stem cell features sorting the cells expressing CXCR4(+)CD133(+) within ovarian cancer cell lines. The sorted population CD133(+)CXCR4(+) demonstrated the highest efficiency in sphere formation in OVCAR-3, OVCAR-4 and OVCAR-5 cells. Moreover OCT4, SOX2, KLF4 and NANOG were highly expressed in CD133(+)CXCR4(+) sorted OVCAR-5 cells. Most strikingly CXCR4(+)CD133(+) sorted OVCAR-5 and -4 cells formed the highest number of tumors when inoculated in nude mice compared to CD133(−)CXCR4(−), CD133(+)CXCR4(−), CD133(−)CXCR4(+) cells. CXCR4(+)CD133(+) OVCAR-5 cells were resistant to cisplatin, overexpressed the ABCG2 surface drug transporter and migrated toward the CXCR4 ligand, CXCL12. Moreover, when human ovarian cancer cells were isolated from 37 primary ovarian cancer, an extremely variable level of CXCR4 and CD133 expression was detected. Thus, in human ovarian cancer cells CXCR4 and CD133 expression identified a discrete population with stem cell properties that regulated tumor development and chemo resistance. This cell population represents a potential therapeutic target.
format Online
Article
Text
id pubmed-4650662
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-46506622015-11-24 Identification of a distinct population of CD133(+)CXCR4(+) cancer stem cells in ovarian cancer Cioffi, Michele D’Alterio, Crescenzo Camerlingo, Rosalba Tirino, Virginia Consales, Claudia Riccio, Anna Ieranò, Caterina Cecere, Sabrina Chiara Losito, Nunzia Simona Greggi, Stefano Pignata, Sandro Pirozzi, Giuseppe Scala, Stefania Sci Rep Article CD133 and CXCR4 were evaluated in the NCI-60 cell lines to identify cancer stem cell rich populations. Screening revealed that, ovarian OVCAR-3, -4 and -5 and colon cancer HT-29, HCT-116 and SW620 over expressed both proteins. We aimed to isolate cells with stem cell features sorting the cells expressing CXCR4(+)CD133(+) within ovarian cancer cell lines. The sorted population CD133(+)CXCR4(+) demonstrated the highest efficiency in sphere formation in OVCAR-3, OVCAR-4 and OVCAR-5 cells. Moreover OCT4, SOX2, KLF4 and NANOG were highly expressed in CD133(+)CXCR4(+) sorted OVCAR-5 cells. Most strikingly CXCR4(+)CD133(+) sorted OVCAR-5 and -4 cells formed the highest number of tumors when inoculated in nude mice compared to CD133(−)CXCR4(−), CD133(+)CXCR4(−), CD133(−)CXCR4(+) cells. CXCR4(+)CD133(+) OVCAR-5 cells were resistant to cisplatin, overexpressed the ABCG2 surface drug transporter and migrated toward the CXCR4 ligand, CXCL12. Moreover, when human ovarian cancer cells were isolated from 37 primary ovarian cancer, an extremely variable level of CXCR4 and CD133 expression was detected. Thus, in human ovarian cancer cells CXCR4 and CD133 expression identified a discrete population with stem cell properties that regulated tumor development and chemo resistance. This cell population represents a potential therapeutic target. Nature Publishing Group 2015-05-28 /pmc/articles/PMC4650662/ /pubmed/26020117 http://dx.doi.org/10.1038/srep10357 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Cioffi, Michele
D’Alterio, Crescenzo
Camerlingo, Rosalba
Tirino, Virginia
Consales, Claudia
Riccio, Anna
Ieranò, Caterina
Cecere, Sabrina Chiara
Losito, Nunzia Simona
Greggi, Stefano
Pignata, Sandro
Pirozzi, Giuseppe
Scala, Stefania
Identification of a distinct population of CD133(+)CXCR4(+) cancer stem cells in ovarian cancer
title Identification of a distinct population of CD133(+)CXCR4(+) cancer stem cells in ovarian cancer
title_full Identification of a distinct population of CD133(+)CXCR4(+) cancer stem cells in ovarian cancer
title_fullStr Identification of a distinct population of CD133(+)CXCR4(+) cancer stem cells in ovarian cancer
title_full_unstemmed Identification of a distinct population of CD133(+)CXCR4(+) cancer stem cells in ovarian cancer
title_short Identification of a distinct population of CD133(+)CXCR4(+) cancer stem cells in ovarian cancer
title_sort identification of a distinct population of cd133(+)cxcr4(+) cancer stem cells in ovarian cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4650662/
https://www.ncbi.nlm.nih.gov/pubmed/26020117
http://dx.doi.org/10.1038/srep10357
work_keys_str_mv AT cioffimichele identificationofadistinctpopulationofcd133cxcr4cancerstemcellsinovariancancer
AT dalteriocrescenzo identificationofadistinctpopulationofcd133cxcr4cancerstemcellsinovariancancer
AT camerlingorosalba identificationofadistinctpopulationofcd133cxcr4cancerstemcellsinovariancancer
AT tirinovirginia identificationofadistinctpopulationofcd133cxcr4cancerstemcellsinovariancancer
AT consalesclaudia identificationofadistinctpopulationofcd133cxcr4cancerstemcellsinovariancancer
AT riccioanna identificationofadistinctpopulationofcd133cxcr4cancerstemcellsinovariancancer
AT ieranocaterina identificationofadistinctpopulationofcd133cxcr4cancerstemcellsinovariancancer
AT ceceresabrinachiara identificationofadistinctpopulationofcd133cxcr4cancerstemcellsinovariancancer
AT lositonunziasimona identificationofadistinctpopulationofcd133cxcr4cancerstemcellsinovariancancer
AT greggistefano identificationofadistinctpopulationofcd133cxcr4cancerstemcellsinovariancancer
AT pignatasandro identificationofadistinctpopulationofcd133cxcr4cancerstemcellsinovariancancer
AT pirozzigiuseppe identificationofadistinctpopulationofcd133cxcr4cancerstemcellsinovariancancer
AT scalastefania identificationofadistinctpopulationofcd133cxcr4cancerstemcellsinovariancancer