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Isolation of nuclear microsatellite markers for Cyperus fuscus (Cyperaceae)(1)

PREMISE OF THE STUDY: Microsatellite markers were characterized in the extremely specialized ephemeral wetland plant species Cyperus fuscus (Cyperaceae). The markers will be used for studying population genetics in natural vs. anthropogenic habitats, on a European scale, and the role of the soil see...

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Autores principales: Böckelmann, Jörg, Wieser, David, Tremetsberger, Karin, Šumberová, Kateřina, Bernhardt, Karl-Georg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4651633/
https://www.ncbi.nlm.nih.gov/pubmed/26649269
http://dx.doi.org/10.3732/apps.1500071
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author Böckelmann, Jörg
Wieser, David
Tremetsberger, Karin
Šumberová, Kateřina
Bernhardt, Karl-Georg
author_facet Böckelmann, Jörg
Wieser, David
Tremetsberger, Karin
Šumberová, Kateřina
Bernhardt, Karl-Georg
author_sort Böckelmann, Jörg
collection PubMed
description PREMISE OF THE STUDY: Microsatellite markers were characterized in the extremely specialized ephemeral wetland plant species Cyperus fuscus (Cyperaceae). The markers will be used for studying population genetics in natural vs. anthropogenic habitats, on a European scale, and the role of the soil seed bank in the life cycle of this ephemeral species. METHODS AND RESULTS: Twenty-one microsatellite loci were established and scored in two populations, with mean number of alleles of 2.6 and 2.9 and mean expected heterozygosity of 0.405 and 0.470, respectively. Forty-four additional loci with the number of alleles ranging from one to four (mean = 2.1) were successfully amplified in seven individuals. CONCLUSIONS: The novel microsatellite markers will be useful for studying the genetic structure of populations of this ephemeral plant as well as their seed bank.
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spelling pubmed-46516332015-12-08 Isolation of nuclear microsatellite markers for Cyperus fuscus (Cyperaceae)(1) Böckelmann, Jörg Wieser, David Tremetsberger, Karin Šumberová, Kateřina Bernhardt, Karl-Georg Appl Plant Sci Primer Note PREMISE OF THE STUDY: Microsatellite markers were characterized in the extremely specialized ephemeral wetland plant species Cyperus fuscus (Cyperaceae). The markers will be used for studying population genetics in natural vs. anthropogenic habitats, on a European scale, and the role of the soil seed bank in the life cycle of this ephemeral species. METHODS AND RESULTS: Twenty-one microsatellite loci were established and scored in two populations, with mean number of alleles of 2.6 and 2.9 and mean expected heterozygosity of 0.405 and 0.470, respectively. Forty-four additional loci with the number of alleles ranging from one to four (mean = 2.1) were successfully amplified in seven individuals. CONCLUSIONS: The novel microsatellite markers will be useful for studying the genetic structure of populations of this ephemeral plant as well as their seed bank. Botanical Society of America 2015-11-04 /pmc/articles/PMC4651633/ /pubmed/26649269 http://dx.doi.org/10.3732/apps.1500071 Text en © 2015 Böckelmann et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
spellingShingle Primer Note
Böckelmann, Jörg
Wieser, David
Tremetsberger, Karin
Šumberová, Kateřina
Bernhardt, Karl-Georg
Isolation of nuclear microsatellite markers for Cyperus fuscus (Cyperaceae)(1)
title Isolation of nuclear microsatellite markers for Cyperus fuscus (Cyperaceae)(1)
title_full Isolation of nuclear microsatellite markers for Cyperus fuscus (Cyperaceae)(1)
title_fullStr Isolation of nuclear microsatellite markers for Cyperus fuscus (Cyperaceae)(1)
title_full_unstemmed Isolation of nuclear microsatellite markers for Cyperus fuscus (Cyperaceae)(1)
title_short Isolation of nuclear microsatellite markers for Cyperus fuscus (Cyperaceae)(1)
title_sort isolation of nuclear microsatellite markers for cyperus fuscus (cyperaceae)(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4651633/
https://www.ncbi.nlm.nih.gov/pubmed/26649269
http://dx.doi.org/10.3732/apps.1500071
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