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An Efficient Site-Specific Method for Irreversible Covalent Labeling of Proteins with a Fluorophore

Fluorophore labeling of proteins while preserving native functions is essential for bulk Förster resonance energy transfer (FRET) interaction and single molecule imaging analysis. Here we describe a versatile, efficient, specific, irreversible, gentle and low-cost method for labeling proteins with f...

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Detalles Bibliográficos
Autores principales: Liu, Jiaquan, Hanne, Jeungphill, Britton, Brooke M., Shoffner, Matthew, Albers, Aaron E., Bennett, Jared, Zatezalo, Rachel, Barfield, Robyn, Rabuka, David, Lee, Jong-Bong, Fishel, Richard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4652282/
https://www.ncbi.nlm.nih.gov/pubmed/26582263
http://dx.doi.org/10.1038/srep16883

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