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An Efficient Site-Specific Method for Irreversible Covalent Labeling of Proteins with a Fluorophore
Fluorophore labeling of proteins while preserving native functions is essential for bulk Förster resonance energy transfer (FRET) interaction and single molecule imaging analysis. Here we describe a versatile, efficient, specific, irreversible, gentle and low-cost method for labeling proteins with f...
Autores principales: | Liu, Jiaquan, Hanne, Jeungphill, Britton, Brooke M., Shoffner, Matthew, Albers, Aaron E., Bennett, Jared, Zatezalo, Rachel, Barfield, Robyn, Rabuka, David, Lee, Jong-Bong, Fishel, Richard |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4652282/ https://www.ncbi.nlm.nih.gov/pubmed/26582263 http://dx.doi.org/10.1038/srep16883 |
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