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Segmental paleotetraploidy revealed in sterlet (Acipenser ruthenus) genome by chromosome painting

BACKGROUND: Acipenseriformes take a basal position among Actinopteri and demonstrate a striking ploidy variation among species. The sterlet (Acipenser ruthenus, Linnaeus, 1758; ARUT) is a diploid 120-chromosomal sturgeon distributed in Eurasian rivers from Danube to Enisey. Despite a high commercial...

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Autores principales: Romanenko, Svetlana A., Biltueva, Larisa S., Serdyukova, Natalya A., Kulemzina, Anastasia I., Beklemisheva, Violetta R., Gladkikh, Olga L., Lemskaya, Natalia A., Interesova, Elena A., Korentovich, Marina A., Vorobieva, Nadezhda V., Graphodatsky, Alexander S., Trifonov, Vladimir A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4652396/
https://www.ncbi.nlm.nih.gov/pubmed/26587056
http://dx.doi.org/10.1186/s13039-015-0194-8
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author Romanenko, Svetlana A.
Biltueva, Larisa S.
Serdyukova, Natalya A.
Kulemzina, Anastasia I.
Beklemisheva, Violetta R.
Gladkikh, Olga L.
Lemskaya, Natalia A.
Interesova, Elena A.
Korentovich, Marina A.
Vorobieva, Nadezhda V.
Graphodatsky, Alexander S.
Trifonov, Vladimir A.
author_facet Romanenko, Svetlana A.
Biltueva, Larisa S.
Serdyukova, Natalya A.
Kulemzina, Anastasia I.
Beklemisheva, Violetta R.
Gladkikh, Olga L.
Lemskaya, Natalia A.
Interesova, Elena A.
Korentovich, Marina A.
Vorobieva, Nadezhda V.
Graphodatsky, Alexander S.
Trifonov, Vladimir A.
author_sort Romanenko, Svetlana A.
collection PubMed
description BACKGROUND: Acipenseriformes take a basal position among Actinopteri and demonstrate a striking ploidy variation among species. The sterlet (Acipenser ruthenus, Linnaeus, 1758; ARUT) is a diploid 120-chromosomal sturgeon distributed in Eurasian rivers from Danube to Enisey. Despite a high commercial value and a rapid population decline in the wild, many genomic characteristics of sterlet (as well as many other sturgeon species) have not been studied. RESULTS: Cell lines from different tissues of 12 sterlet specimens from Siberian populations were established following an optimized protocol. Conventional cytogenetic studies supplemented with molecular cytogenetic investigations on obtained fibroblast cell lines allowed a detailed description of sterlet karyotype and a precise localization of 18S/28S and 5S ribosomal clusters. Localization of sturgeon specific HindIII repetitive elements revealed an increased concentration in the pericentromeric region of the acrocentric ARUT14, while the total sterlet repetitive DNA fraction (C(0)t30) produced bright signals on subtelomeric segments of small chromosomal elements. Chromosome and region specific probes ARUT1p, 5, 6, 7, 8 as well as 14 anonymous small sized chromosomes (probes A-N) generated by microdissection were applied in chromosome painting experiments. According to hybridization patterns all painting probes were classified into two major groups: the first group (ARUT5, 6, 8 as well as microchromosome specific probes C, E, F, G, H, and I) painted only a single region each on sterlet metaphases, while probes of the second group (ARUT1p, 7 as well as microchromosome derived probes A, B, D, J, K, M, and N) marked two genomic segments each on different chromosomes. Similar results were obtained on male and female metaphases. CONCLUSIONS: The sterlet genome represents a complex mosaic structure and consists of diploid and tetraploid chromosome segments. This may be regarded as a transition stage from paleotetraploid (functional diploid) to diploid genome condition. Molecular cytogenetic and genomic studies of other 120- and 240-chromosomal sturgeons are needed to reconstruct genome evolution of this vertebrate group.
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spelling pubmed-46523962015-11-20 Segmental paleotetraploidy revealed in sterlet (Acipenser ruthenus) genome by chromosome painting Romanenko, Svetlana A. Biltueva, Larisa S. Serdyukova, Natalya A. Kulemzina, Anastasia I. Beklemisheva, Violetta R. Gladkikh, Olga L. Lemskaya, Natalia A. Interesova, Elena A. Korentovich, Marina A. Vorobieva, Nadezhda V. Graphodatsky, Alexander S. Trifonov, Vladimir A. Mol Cytogenet Research BACKGROUND: Acipenseriformes take a basal position among Actinopteri and demonstrate a striking ploidy variation among species. The sterlet (Acipenser ruthenus, Linnaeus, 1758; ARUT) is a diploid 120-chromosomal sturgeon distributed in Eurasian rivers from Danube to Enisey. Despite a high commercial value and a rapid population decline in the wild, many genomic characteristics of sterlet (as well as many other sturgeon species) have not been studied. RESULTS: Cell lines from different tissues of 12 sterlet specimens from Siberian populations were established following an optimized protocol. Conventional cytogenetic studies supplemented with molecular cytogenetic investigations on obtained fibroblast cell lines allowed a detailed description of sterlet karyotype and a precise localization of 18S/28S and 5S ribosomal clusters. Localization of sturgeon specific HindIII repetitive elements revealed an increased concentration in the pericentromeric region of the acrocentric ARUT14, while the total sterlet repetitive DNA fraction (C(0)t30) produced bright signals on subtelomeric segments of small chromosomal elements. Chromosome and region specific probes ARUT1p, 5, 6, 7, 8 as well as 14 anonymous small sized chromosomes (probes A-N) generated by microdissection were applied in chromosome painting experiments. According to hybridization patterns all painting probes were classified into two major groups: the first group (ARUT5, 6, 8 as well as microchromosome specific probes C, E, F, G, H, and I) painted only a single region each on sterlet metaphases, while probes of the second group (ARUT1p, 7 as well as microchromosome derived probes A, B, D, J, K, M, and N) marked two genomic segments each on different chromosomes. Similar results were obtained on male and female metaphases. CONCLUSIONS: The sterlet genome represents a complex mosaic structure and consists of diploid and tetraploid chromosome segments. This may be regarded as a transition stage from paleotetraploid (functional diploid) to diploid genome condition. Molecular cytogenetic and genomic studies of other 120- and 240-chromosomal sturgeons are needed to reconstruct genome evolution of this vertebrate group. BioMed Central 2015-11-19 /pmc/articles/PMC4652396/ /pubmed/26587056 http://dx.doi.org/10.1186/s13039-015-0194-8 Text en © Romanenko et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Romanenko, Svetlana A.
Biltueva, Larisa S.
Serdyukova, Natalya A.
Kulemzina, Anastasia I.
Beklemisheva, Violetta R.
Gladkikh, Olga L.
Lemskaya, Natalia A.
Interesova, Elena A.
Korentovich, Marina A.
Vorobieva, Nadezhda V.
Graphodatsky, Alexander S.
Trifonov, Vladimir A.
Segmental paleotetraploidy revealed in sterlet (Acipenser ruthenus) genome by chromosome painting
title Segmental paleotetraploidy revealed in sterlet (Acipenser ruthenus) genome by chromosome painting
title_full Segmental paleotetraploidy revealed in sterlet (Acipenser ruthenus) genome by chromosome painting
title_fullStr Segmental paleotetraploidy revealed in sterlet (Acipenser ruthenus) genome by chromosome painting
title_full_unstemmed Segmental paleotetraploidy revealed in sterlet (Acipenser ruthenus) genome by chromosome painting
title_short Segmental paleotetraploidy revealed in sterlet (Acipenser ruthenus) genome by chromosome painting
title_sort segmental paleotetraploidy revealed in sterlet (acipenser ruthenus) genome by chromosome painting
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4652396/
https://www.ncbi.nlm.nih.gov/pubmed/26587056
http://dx.doi.org/10.1186/s13039-015-0194-8
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