Heterologous protein production using euchromatin-containing expression vectors in mammalian cells

Upon stable cell line generation, chromosomal integration site of the vector DNA has a major impact on transgene expression. Here we apply an active gene environment, rather than specified genetic elements, in expression vectors used for random integration. We generated a set of Bacterial Artificial...

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Detalles Bibliográficos
Autores principales: Zboray, Katalin, Sommeregger, Wolfgang, Bogner, Edith, Gili, Andreas, Sterovsky, Thomas, Fauland, Katharina, Grabner, Beatrice, Stiedl, Patricia, Moll, Herwig P., Bauer, Anton, Kunert, Renate, Casanova, Emilio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4652741/
https://www.ncbi.nlm.nih.gov/pubmed/25977298
http://dx.doi.org/10.1093/nar/gkv475

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