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Enhanced antitumor efficacy of ultrasonic cavitation with up-sized microbubbles in pancreatic cancer
Ultrasonic cavitation is a novel potential approach for cancer treatment. We optimized the techniques of ultrasonic cavitation to enhance antitumor efficacy in a mouse model with human pancreatic cancer. A polydisperse MB contrast agent formulation (TS-P) with a mean number diameter of 1.9 μm was de...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4653001/ https://www.ncbi.nlm.nih.gov/pubmed/26036312 |
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author | Huang, Pintong Zhang, Ying Chen, Jian Shentu, Weihui Sun, Yu Yang, Zhijian Liang, Tingbo Chen, Shuyuan Pu, Zhaoxia |
author_facet | Huang, Pintong Zhang, Ying Chen, Jian Shentu, Weihui Sun, Yu Yang, Zhijian Liang, Tingbo Chen, Shuyuan Pu, Zhaoxia |
author_sort | Huang, Pintong |
collection | PubMed |
description | Ultrasonic cavitation is a novel potential approach for cancer treatment. We optimized the techniques of ultrasonic cavitation to enhance antitumor efficacy in a mouse model with human pancreatic cancer. A polydisperse MB contrast agent formulation (TS-P) with a mean number diameter of 1.9 μm was depleted in small diameter particles by differential centrifugation, producing an “up-sized” size distribution (TS-PL) possessing a mean diameter of 2.9 μm. Mice bearing the XPA-1-RFP pancreatic tumor were treated daily for 3 consecutive days with either up-sized or standard MB. Both treatment cohorts exhibited a significant reduction in tumor volume relative to the untreated control cohort (P < 0.05), and TS-PL group has significantly reduction in tumor volume (1215.1± 324.7 mm(3)) compared with standard TS-P group (2131.2±753.4 mm(3)) (P < 0.05). The treatment with TS-PL resulted in more tumor cell necrosis and apoptosis than with TS-P. Decreased expression of CD31 and MVD was observed histologically in tumors treated with TS-PL relative to TS-P. This study demonstrates that tuning the size distribution of existing contrast agent products, specifically to reduce the concentration of small MB, is required for enhanced anti-tumor cavitation activity. |
format | Online Article Text |
id | pubmed-4653001 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-46530012015-12-02 Enhanced antitumor efficacy of ultrasonic cavitation with up-sized microbubbles in pancreatic cancer Huang, Pintong Zhang, Ying Chen, Jian Shentu, Weihui Sun, Yu Yang, Zhijian Liang, Tingbo Chen, Shuyuan Pu, Zhaoxia Oncotarget Research Paper Ultrasonic cavitation is a novel potential approach for cancer treatment. We optimized the techniques of ultrasonic cavitation to enhance antitumor efficacy in a mouse model with human pancreatic cancer. A polydisperse MB contrast agent formulation (TS-P) with a mean number diameter of 1.9 μm was depleted in small diameter particles by differential centrifugation, producing an “up-sized” size distribution (TS-PL) possessing a mean diameter of 2.9 μm. Mice bearing the XPA-1-RFP pancreatic tumor were treated daily for 3 consecutive days with either up-sized or standard MB. Both treatment cohorts exhibited a significant reduction in tumor volume relative to the untreated control cohort (P < 0.05), and TS-PL group has significantly reduction in tumor volume (1215.1± 324.7 mm(3)) compared with standard TS-P group (2131.2±753.4 mm(3)) (P < 0.05). The treatment with TS-PL resulted in more tumor cell necrosis and apoptosis than with TS-P. Decreased expression of CD31 and MVD was observed histologically in tumors treated with TS-PL relative to TS-P. This study demonstrates that tuning the size distribution of existing contrast agent products, specifically to reduce the concentration of small MB, is required for enhanced anti-tumor cavitation activity. Impact Journals LLC 2015-05-08 /pmc/articles/PMC4653001/ /pubmed/26036312 Text en Copyright: © 2015 Huang et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Huang, Pintong Zhang, Ying Chen, Jian Shentu, Weihui Sun, Yu Yang, Zhijian Liang, Tingbo Chen, Shuyuan Pu, Zhaoxia Enhanced antitumor efficacy of ultrasonic cavitation with up-sized microbubbles in pancreatic cancer |
title | Enhanced antitumor efficacy of ultrasonic cavitation with up-sized microbubbles in pancreatic cancer |
title_full | Enhanced antitumor efficacy of ultrasonic cavitation with up-sized microbubbles in pancreatic cancer |
title_fullStr | Enhanced antitumor efficacy of ultrasonic cavitation with up-sized microbubbles in pancreatic cancer |
title_full_unstemmed | Enhanced antitumor efficacy of ultrasonic cavitation with up-sized microbubbles in pancreatic cancer |
title_short | Enhanced antitumor efficacy of ultrasonic cavitation with up-sized microbubbles in pancreatic cancer |
title_sort | enhanced antitumor efficacy of ultrasonic cavitation with up-sized microbubbles in pancreatic cancer |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4653001/ https://www.ncbi.nlm.nih.gov/pubmed/26036312 |
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