Cargando…
Snail and serpinA1 promote tumor progression and predict prognosis in colorectal cancer
The role of Snail and serpin peptidase inhibitor clade A member 1 (serpinA1) in tumorigenesis has been previously identified. However, the exact role and mechanism of these proteins in progression of colorectal cancer (CRC) are controversial. In this study, we investigated the role of Snail and serp...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4653007/ https://www.ncbi.nlm.nih.gov/pubmed/26015410 |
_version_ | 1782401852463120384 |
---|---|
author | Kwon, Chae Hwa Park, Hye Ji Choi, Jin Hwa Lee, Ja Rang Kim, Hye Kyung Jo, Hong-jae Kim, Hyun Sung Oh, Nahmgun Song, Geun Am Park, Do Youn |
author_facet | Kwon, Chae Hwa Park, Hye Ji Choi, Jin Hwa Lee, Ja Rang Kim, Hye Kyung Jo, Hong-jae Kim, Hyun Sung Oh, Nahmgun Song, Geun Am Park, Do Youn |
author_sort | Kwon, Chae Hwa |
collection | PubMed |
description | The role of Snail and serpin peptidase inhibitor clade A member 1 (serpinA1) in tumorigenesis has been previously identified. However, the exact role and mechanism of these proteins in progression of colorectal cancer (CRC) are controversial. In this study, we investigated the role of Snail and serpinA1 in colorectal cancer (CRC) and examined the mechanisms through which these proteins mediate CRC progression. Immunohistochemical analysis of 528 samples from patients with CRC showed that elevated expression of Snail or serpinA1 was correlated with advanced stage, lymph node metastasis, and poor prognosis. Moreover, we detected a correlation between Snail and serpinA1 expression. Functional studies performed using the CRC cell lines DLD-1 and SW-480 showed that overexpression of Snail or serpinA1 significantly increased CRC cell invasion and migration. Conversely, knockdown of Snail or serpinA1 expression suppressed CRC cell invasion and migration. ChIP analysis revealed that Snail regulated serpinA1 by binding to its promoter. In addition, fibronectin mediated Snail and serpinA1 signaling was involved in CRC cell invasion and migration. Taken together, our data showed that Snail and serpinA1 promoted CRC progression through fibronectin. These findings suggested that Snail and serpinA1 were novel prognostic biomarkers and candidate therapeutic targets in CRC. |
format | Online Article Text |
id | pubmed-4653007 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-46530072015-12-02 Snail and serpinA1 promote tumor progression and predict prognosis in colorectal cancer Kwon, Chae Hwa Park, Hye Ji Choi, Jin Hwa Lee, Ja Rang Kim, Hye Kyung Jo, Hong-jae Kim, Hyun Sung Oh, Nahmgun Song, Geun Am Park, Do Youn Oncotarget Research Paper The role of Snail and serpin peptidase inhibitor clade A member 1 (serpinA1) in tumorigenesis has been previously identified. However, the exact role and mechanism of these proteins in progression of colorectal cancer (CRC) are controversial. In this study, we investigated the role of Snail and serpinA1 in colorectal cancer (CRC) and examined the mechanisms through which these proteins mediate CRC progression. Immunohistochemical analysis of 528 samples from patients with CRC showed that elevated expression of Snail or serpinA1 was correlated with advanced stage, lymph node metastasis, and poor prognosis. Moreover, we detected a correlation between Snail and serpinA1 expression. Functional studies performed using the CRC cell lines DLD-1 and SW-480 showed that overexpression of Snail or serpinA1 significantly increased CRC cell invasion and migration. Conversely, knockdown of Snail or serpinA1 expression suppressed CRC cell invasion and migration. ChIP analysis revealed that Snail regulated serpinA1 by binding to its promoter. In addition, fibronectin mediated Snail and serpinA1 signaling was involved in CRC cell invasion and migration. Taken together, our data showed that Snail and serpinA1 promoted CRC progression through fibronectin. These findings suggested that Snail and serpinA1 were novel prognostic biomarkers and candidate therapeutic targets in CRC. Impact Journals LLC 2015-04-29 /pmc/articles/PMC4653007/ /pubmed/26015410 Text en Copyright: © 2015 Kwon et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Kwon, Chae Hwa Park, Hye Ji Choi, Jin Hwa Lee, Ja Rang Kim, Hye Kyung Jo, Hong-jae Kim, Hyun Sung Oh, Nahmgun Song, Geun Am Park, Do Youn Snail and serpinA1 promote tumor progression and predict prognosis in colorectal cancer |
title | Snail and serpinA1 promote tumor progression and predict prognosis in colorectal cancer |
title_full | Snail and serpinA1 promote tumor progression and predict prognosis in colorectal cancer |
title_fullStr | Snail and serpinA1 promote tumor progression and predict prognosis in colorectal cancer |
title_full_unstemmed | Snail and serpinA1 promote tumor progression and predict prognosis in colorectal cancer |
title_short | Snail and serpinA1 promote tumor progression and predict prognosis in colorectal cancer |
title_sort | snail and serpina1 promote tumor progression and predict prognosis in colorectal cancer |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4653007/ https://www.ncbi.nlm.nih.gov/pubmed/26015410 |
work_keys_str_mv | AT kwonchaehwa snailandserpina1promotetumorprogressionandpredictprognosisincolorectalcancer AT parkhyeji snailandserpina1promotetumorprogressionandpredictprognosisincolorectalcancer AT choijinhwa snailandserpina1promotetumorprogressionandpredictprognosisincolorectalcancer AT leejarang snailandserpina1promotetumorprogressionandpredictprognosisincolorectalcancer AT kimhyekyung snailandserpina1promotetumorprogressionandpredictprognosisincolorectalcancer AT johongjae snailandserpina1promotetumorprogressionandpredictprognosisincolorectalcancer AT kimhyunsung snailandserpina1promotetumorprogressionandpredictprognosisincolorectalcancer AT ohnahmgun snailandserpina1promotetumorprogressionandpredictprognosisincolorectalcancer AT songgeunam snailandserpina1promotetumorprogressionandpredictprognosisincolorectalcancer AT parkdoyoun snailandserpina1promotetumorprogressionandpredictprognosisincolorectalcancer |