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Micropropagation of Agave salmiana: Means to Production of Antioxidant and Bioactive Principles

Maguey, Agave salmiana, is an important plant for the “pulque” beverage and functional food industries; however, it has several constraints for elite and homogeneous plant production. In this study, a micropropagation process was established to generate in vitro plants. The effect of the method on m...

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Autores principales: Puente-Garza, César A., Gutiérrez-Mora, Antonia, García-Lara, Silverio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4655248/
https://www.ncbi.nlm.nih.gov/pubmed/26635850
http://dx.doi.org/10.3389/fpls.2015.01026
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author Puente-Garza, César A.
Gutiérrez-Mora, Antonia
García-Lara, Silverio
author_facet Puente-Garza, César A.
Gutiérrez-Mora, Antonia
García-Lara, Silverio
author_sort Puente-Garza, César A.
collection PubMed
description Maguey, Agave salmiana, is an important plant for the “pulque” beverage and functional food industries; however, it has several constraints for elite and homogeneous plant production. In this study, a micropropagation process was established to generate in vitro plants. The effect of the method on metabolite content and antioxidant (AOX) activity in regenerated plants was evaluated. Young germinated plantlets were micropropagated from axillary shoots using Murashige and Skoog medium supplemented with L2 vitamins, 0.04 mg/L 2,4-dichlorophenoxyacetic acid and 10 mg/L 6-benzylaminopurine. Total soluble sugars from the aqueous fraction and total phenolic acids, total saponins, and AOX activity of the methanol fraction were determined in wild-type (WT) plants, in in vitro (IN) plants, and ex vitro acclimated plants (EN). The results showed that IN plants have a 50% lower soluble sugar content compared to WT, and EN. The total phenolic acids content was at least 30% higher in micropropagated (IN) and regenerated (EN) plants compared to WT. The total saponin content in IN, and EN plants was 36 and 25 times higher compared to WT. The AOX capacity of IN plants was on average three times higher compared to other treatments. However, no correlation was found between the AOX activity and total phenolic acids or total saponins. A negative and significant correlation (r = –0.927; p = 0.003) was found between the AOX activity and the total soluble sugars content. Micropropagated plants of A. salmiana have a different phytochemical content and bioactivity after the in vitro process compared to WT plants. The micropropagation process could be used as a platform for phytochemical enhancement of Agave plants.
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spelling pubmed-46552482015-12-03 Micropropagation of Agave salmiana: Means to Production of Antioxidant and Bioactive Principles Puente-Garza, César A. Gutiérrez-Mora, Antonia García-Lara, Silverio Front Plant Sci Plant Science Maguey, Agave salmiana, is an important plant for the “pulque” beverage and functional food industries; however, it has several constraints for elite and homogeneous plant production. In this study, a micropropagation process was established to generate in vitro plants. The effect of the method on metabolite content and antioxidant (AOX) activity in regenerated plants was evaluated. Young germinated plantlets were micropropagated from axillary shoots using Murashige and Skoog medium supplemented with L2 vitamins, 0.04 mg/L 2,4-dichlorophenoxyacetic acid and 10 mg/L 6-benzylaminopurine. Total soluble sugars from the aqueous fraction and total phenolic acids, total saponins, and AOX activity of the methanol fraction were determined in wild-type (WT) plants, in in vitro (IN) plants, and ex vitro acclimated plants (EN). The results showed that IN plants have a 50% lower soluble sugar content compared to WT, and EN. The total phenolic acids content was at least 30% higher in micropropagated (IN) and regenerated (EN) plants compared to WT. The total saponin content in IN, and EN plants was 36 and 25 times higher compared to WT. The AOX capacity of IN plants was on average three times higher compared to other treatments. However, no correlation was found between the AOX activity and total phenolic acids or total saponins. A negative and significant correlation (r = –0.927; p = 0.003) was found between the AOX activity and the total soluble sugars content. Micropropagated plants of A. salmiana have a different phytochemical content and bioactivity after the in vitro process compared to WT plants. The micropropagation process could be used as a platform for phytochemical enhancement of Agave plants. Frontiers Media S.A. 2015-11-23 /pmc/articles/PMC4655248/ /pubmed/26635850 http://dx.doi.org/10.3389/fpls.2015.01026 Text en Copyright © 2015 Puente-Garza, Gutiérrez-Mora and García-Lara. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Puente-Garza, César A.
Gutiérrez-Mora, Antonia
García-Lara, Silverio
Micropropagation of Agave salmiana: Means to Production of Antioxidant and Bioactive Principles
title Micropropagation of Agave salmiana: Means to Production of Antioxidant and Bioactive Principles
title_full Micropropagation of Agave salmiana: Means to Production of Antioxidant and Bioactive Principles
title_fullStr Micropropagation of Agave salmiana: Means to Production of Antioxidant and Bioactive Principles
title_full_unstemmed Micropropagation of Agave salmiana: Means to Production of Antioxidant and Bioactive Principles
title_short Micropropagation of Agave salmiana: Means to Production of Antioxidant and Bioactive Principles
title_sort micropropagation of agave salmiana: means to production of antioxidant and bioactive principles
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4655248/
https://www.ncbi.nlm.nih.gov/pubmed/26635850
http://dx.doi.org/10.3389/fpls.2015.01026
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