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Targeted Large-Scale Deletion of Bacterial Genomes Using CRISPR-Nickases

[Image: see text] Programmable CRISPR-Cas systems have augmented our ability to produce precise genome manipulations. Here we demonstrate and characterize the ability of CRISPR-Cas derived nickases to direct targeted recombination of both small and large genomic regions flanked by repetitive element...

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Detalles Bibliográficos
Autores principales:	Standage-Beier, Kylie, Zhang, Qi, Wang, Xiao
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2015
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4655420/ https://www.ncbi.nlm.nih.gov/pubmed/26451892 http://dx.doi.org/10.1021/acssynbio.5b00132

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