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Laboratory-based nationwide surveillance of antimicrobial resistance in Ghana

Global efforts are underway to combat antimicrobial resistance (AMR). A key target in this intervention is surveillance for local and national action. Data on AMR in Ghana are limited, and monitoring of AMR is nonexistent. We sought to generate baseline data on AMR, and to assess the readiness of Gh...

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Autores principales: Opintan, Japheth A, Newman, Mercy J, Arhin, Reuben E, Donkor, Eric S, Gyansa-Lutterodt, Martha, Mills-Pappoe, William
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4655947/
https://www.ncbi.nlm.nih.gov/pubmed/26604806
http://dx.doi.org/10.2147/IDR.S88725
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author Opintan, Japheth A
Newman, Mercy J
Arhin, Reuben E
Donkor, Eric S
Gyansa-Lutterodt, Martha
Mills-Pappoe, William
author_facet Opintan, Japheth A
Newman, Mercy J
Arhin, Reuben E
Donkor, Eric S
Gyansa-Lutterodt, Martha
Mills-Pappoe, William
author_sort Opintan, Japheth A
collection PubMed
description Global efforts are underway to combat antimicrobial resistance (AMR). A key target in this intervention is surveillance for local and national action. Data on AMR in Ghana are limited, and monitoring of AMR is nonexistent. We sought to generate baseline data on AMR, and to assess the readiness of Ghana in laboratory-based surveillance. Biomedical scientists in laboratories across Ghana with capacity to perform bacteriological culture were selected and trained. In-house standard operating protocols were used to perform microbiological investigations on clinical specimens. Additional microbiological tests and data analyses were performed at a centralized laboratory. Surveillance data were stored and analyzed using WHONET program files. A total of 24 laboratories participated in the training, and 1,598 data sets were included in the final analysis. A majority of the bacterial species were isolated from outpatients (963 isolates; 60.3%). Urine (617 isolates; 38.6%) was the most common clinical specimen cultured, compared to blood (100 isolates; 6.3%). Ten of 18 laboratories performed blood culture. Bacteria isolated included Escherichia coli (27.5%), Pseudomonas spp. (14.0%), Staphylococcus aureus (11.5%), Streptococcus spp. (2.3%), and Salmonella enterica serovar Typhi (0.6%). Most of the isolates were multidrug-resistant, and over 80% of them were extended-spectrum beta-lactamases-producing. Minimum inhibitory concentration levels at 50% and at 90% for ciprofloxacin, ceftriaxone, and amikacin on selected multidrug-resistant bacteria species ranged between 2 µg/mL and >256 µg/mL. A range of clinical bacterial isolates were resistant to important commonly used antimicrobials in the country, necessitating an effective surveillance to continuously monitor AMR in Ghana. With local and international support, Ghana can participate in global AMR surveillance.
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spelling pubmed-46559472015-11-24 Laboratory-based nationwide surveillance of antimicrobial resistance in Ghana Opintan, Japheth A Newman, Mercy J Arhin, Reuben E Donkor, Eric S Gyansa-Lutterodt, Martha Mills-Pappoe, William Infect Drug Resist Original Research Global efforts are underway to combat antimicrobial resistance (AMR). A key target in this intervention is surveillance for local and national action. Data on AMR in Ghana are limited, and monitoring of AMR is nonexistent. We sought to generate baseline data on AMR, and to assess the readiness of Ghana in laboratory-based surveillance. Biomedical scientists in laboratories across Ghana with capacity to perform bacteriological culture were selected and trained. In-house standard operating protocols were used to perform microbiological investigations on clinical specimens. Additional microbiological tests and data analyses were performed at a centralized laboratory. Surveillance data were stored and analyzed using WHONET program files. A total of 24 laboratories participated in the training, and 1,598 data sets were included in the final analysis. A majority of the bacterial species were isolated from outpatients (963 isolates; 60.3%). Urine (617 isolates; 38.6%) was the most common clinical specimen cultured, compared to blood (100 isolates; 6.3%). Ten of 18 laboratories performed blood culture. Bacteria isolated included Escherichia coli (27.5%), Pseudomonas spp. (14.0%), Staphylococcus aureus (11.5%), Streptococcus spp. (2.3%), and Salmonella enterica serovar Typhi (0.6%). Most of the isolates were multidrug-resistant, and over 80% of them were extended-spectrum beta-lactamases-producing. Minimum inhibitory concentration levels at 50% and at 90% for ciprofloxacin, ceftriaxone, and amikacin on selected multidrug-resistant bacteria species ranged between 2 µg/mL and >256 µg/mL. A range of clinical bacterial isolates were resistant to important commonly used antimicrobials in the country, necessitating an effective surveillance to continuously monitor AMR in Ghana. With local and international support, Ghana can participate in global AMR surveillance. Dove Medical Press 2015-11-18 /pmc/articles/PMC4655947/ /pubmed/26604806 http://dx.doi.org/10.2147/IDR.S88725 Text en © 2015 Opintan et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Opintan, Japheth A
Newman, Mercy J
Arhin, Reuben E
Donkor, Eric S
Gyansa-Lutterodt, Martha
Mills-Pappoe, William
Laboratory-based nationwide surveillance of antimicrobial resistance in Ghana
title Laboratory-based nationwide surveillance of antimicrobial resistance in Ghana
title_full Laboratory-based nationwide surveillance of antimicrobial resistance in Ghana
title_fullStr Laboratory-based nationwide surveillance of antimicrobial resistance in Ghana
title_full_unstemmed Laboratory-based nationwide surveillance of antimicrobial resistance in Ghana
title_short Laboratory-based nationwide surveillance of antimicrobial resistance in Ghana
title_sort laboratory-based nationwide surveillance of antimicrobial resistance in ghana
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4655947/
https://www.ncbi.nlm.nih.gov/pubmed/26604806
http://dx.doi.org/10.2147/IDR.S88725
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