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Optical volume and mass measurements show that mammalian cells swell during mitosis

The extent, mechanism, and function of cell volume changes during specific cellular events, such as cell migration and cell division, have been poorly studied, mostly because of a lack of adequate techniques. Here we unambiguously report that a large range of mammalian cell types display a significa...

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Detalles Bibliográficos
Autores principales: Zlotek-Zlotkiewicz, Ewa, Monnier, Sylvain, Cappello, Giovanni, Le Berre, Mael, Piel, Matthieu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4657168/
https://www.ncbi.nlm.nih.gov/pubmed/26598614
http://dx.doi.org/10.1083/jcb.201505056
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author Zlotek-Zlotkiewicz, Ewa
Monnier, Sylvain
Cappello, Giovanni
Le Berre, Mael
Piel, Matthieu
author_facet Zlotek-Zlotkiewicz, Ewa
Monnier, Sylvain
Cappello, Giovanni
Le Berre, Mael
Piel, Matthieu
author_sort Zlotek-Zlotkiewicz, Ewa
collection PubMed
description The extent, mechanism, and function of cell volume changes during specific cellular events, such as cell migration and cell division, have been poorly studied, mostly because of a lack of adequate techniques. Here we unambiguously report that a large range of mammalian cell types display a significant increase in volume during mitosis (up to 30%). We further show that this increase in volume is tightly linked to the mitotic state of the cell and not to its spread or rounded shape and is independent of the presence of an intact actomyosin cortex. Importantly, this volume increase is not accompanied by an increase in dry mass and thus corresponds to a decrease in cell density. This mitotic swelling might have important consequences for mitotic progression: it might contribute to produce strong pushing forces, allowing mitotic cells to round up; it might also, by lowering cytoplasmic density, contribute to the large change of physicochemical properties observed in mitotic cells.
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spelling pubmed-46571682016-05-23 Optical volume and mass measurements show that mammalian cells swell during mitosis Zlotek-Zlotkiewicz, Ewa Monnier, Sylvain Cappello, Giovanni Le Berre, Mael Piel, Matthieu J Cell Biol Research Articles The extent, mechanism, and function of cell volume changes during specific cellular events, such as cell migration and cell division, have been poorly studied, mostly because of a lack of adequate techniques. Here we unambiguously report that a large range of mammalian cell types display a significant increase in volume during mitosis (up to 30%). We further show that this increase in volume is tightly linked to the mitotic state of the cell and not to its spread or rounded shape and is independent of the presence of an intact actomyosin cortex. Importantly, this volume increase is not accompanied by an increase in dry mass and thus corresponds to a decrease in cell density. This mitotic swelling might have important consequences for mitotic progression: it might contribute to produce strong pushing forces, allowing mitotic cells to round up; it might also, by lowering cytoplasmic density, contribute to the large change of physicochemical properties observed in mitotic cells. The Rockefeller University Press 2015-11-23 /pmc/articles/PMC4657168/ /pubmed/26598614 http://dx.doi.org/10.1083/jcb.201505056 Text en © 2015 Zlotek-Zlotkiewicz et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Zlotek-Zlotkiewicz, Ewa
Monnier, Sylvain
Cappello, Giovanni
Le Berre, Mael
Piel, Matthieu
Optical volume and mass measurements show that mammalian cells swell during mitosis
title Optical volume and mass measurements show that mammalian cells swell during mitosis
title_full Optical volume and mass measurements show that mammalian cells swell during mitosis
title_fullStr Optical volume and mass measurements show that mammalian cells swell during mitosis
title_full_unstemmed Optical volume and mass measurements show that mammalian cells swell during mitosis
title_short Optical volume and mass measurements show that mammalian cells swell during mitosis
title_sort optical volume and mass measurements show that mammalian cells swell during mitosis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4657168/
https://www.ncbi.nlm.nih.gov/pubmed/26598614
http://dx.doi.org/10.1083/jcb.201505056
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