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Optical volume and mass measurements show that mammalian cells swell during mitosis
The extent, mechanism, and function of cell volume changes during specific cellular events, such as cell migration and cell division, have been poorly studied, mostly because of a lack of adequate techniques. Here we unambiguously report that a large range of mammalian cell types display a significa...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4657168/ https://www.ncbi.nlm.nih.gov/pubmed/26598614 http://dx.doi.org/10.1083/jcb.201505056 |
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author | Zlotek-Zlotkiewicz, Ewa Monnier, Sylvain Cappello, Giovanni Le Berre, Mael Piel, Matthieu |
author_facet | Zlotek-Zlotkiewicz, Ewa Monnier, Sylvain Cappello, Giovanni Le Berre, Mael Piel, Matthieu |
author_sort | Zlotek-Zlotkiewicz, Ewa |
collection | PubMed |
description | The extent, mechanism, and function of cell volume changes during specific cellular events, such as cell migration and cell division, have been poorly studied, mostly because of a lack of adequate techniques. Here we unambiguously report that a large range of mammalian cell types display a significant increase in volume during mitosis (up to 30%). We further show that this increase in volume is tightly linked to the mitotic state of the cell and not to its spread or rounded shape and is independent of the presence of an intact actomyosin cortex. Importantly, this volume increase is not accompanied by an increase in dry mass and thus corresponds to a decrease in cell density. This mitotic swelling might have important consequences for mitotic progression: it might contribute to produce strong pushing forces, allowing mitotic cells to round up; it might also, by lowering cytoplasmic density, contribute to the large change of physicochemical properties observed in mitotic cells. |
format | Online Article Text |
id | pubmed-4657168 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-46571682016-05-23 Optical volume and mass measurements show that mammalian cells swell during mitosis Zlotek-Zlotkiewicz, Ewa Monnier, Sylvain Cappello, Giovanni Le Berre, Mael Piel, Matthieu J Cell Biol Research Articles The extent, mechanism, and function of cell volume changes during specific cellular events, such as cell migration and cell division, have been poorly studied, mostly because of a lack of adequate techniques. Here we unambiguously report that a large range of mammalian cell types display a significant increase in volume during mitosis (up to 30%). We further show that this increase in volume is tightly linked to the mitotic state of the cell and not to its spread or rounded shape and is independent of the presence of an intact actomyosin cortex. Importantly, this volume increase is not accompanied by an increase in dry mass and thus corresponds to a decrease in cell density. This mitotic swelling might have important consequences for mitotic progression: it might contribute to produce strong pushing forces, allowing mitotic cells to round up; it might also, by lowering cytoplasmic density, contribute to the large change of physicochemical properties observed in mitotic cells. The Rockefeller University Press 2015-11-23 /pmc/articles/PMC4657168/ /pubmed/26598614 http://dx.doi.org/10.1083/jcb.201505056 Text en © 2015 Zlotek-Zlotkiewicz et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Zlotek-Zlotkiewicz, Ewa Monnier, Sylvain Cappello, Giovanni Le Berre, Mael Piel, Matthieu Optical volume and mass measurements show that mammalian cells swell during mitosis |
title | Optical volume and mass measurements show that mammalian cells swell during mitosis |
title_full | Optical volume and mass measurements show that mammalian cells swell during mitosis |
title_fullStr | Optical volume and mass measurements show that mammalian cells swell during mitosis |
title_full_unstemmed | Optical volume and mass measurements show that mammalian cells swell during mitosis |
title_short | Optical volume and mass measurements show that mammalian cells swell during mitosis |
title_sort | optical volume and mass measurements show that mammalian cells swell during mitosis |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4657168/ https://www.ncbi.nlm.nih.gov/pubmed/26598614 http://dx.doi.org/10.1083/jcb.201505056 |
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