Cargando…

Resonant microchannel volume and mass measurements show that suspended cells swell during mitosis

Osmotic regulation of intracellular water during mitosis is poorly understood because methods for monitoring relevant cellular physical properties with sufficient precision have been limited. Here we use a suspended microchannel resonator to monitor the volume and density of single cells in suspensi...

Descripción completa

Detalles Bibliográficos
Autores principales: Son, Sungmin, Kang, Joon Ho, Oh, Seungeun, Kirschner, Marc W., Mitchison, T.J., Manalis, Scott
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4657169/
https://www.ncbi.nlm.nih.gov/pubmed/26598613
http://dx.doi.org/10.1083/jcb.201505058
_version_ 1782402345251897344
author Son, Sungmin
Kang, Joon Ho
Oh, Seungeun
Kirschner, Marc W.
Mitchison, T.J.
Manalis, Scott
author_facet Son, Sungmin
Kang, Joon Ho
Oh, Seungeun
Kirschner, Marc W.
Mitchison, T.J.
Manalis, Scott
author_sort Son, Sungmin
collection PubMed
description Osmotic regulation of intracellular water during mitosis is poorly understood because methods for monitoring relevant cellular physical properties with sufficient precision have been limited. Here we use a suspended microchannel resonator to monitor the volume and density of single cells in suspension with a precision of 1% and 0.03%, respectively. We find that for transformed murine lymphocytic leukemia and mouse pro–B cell lymphoid cell lines, mitotic cells reversibly increase their volume by more than 10% and decrease their density by 0.4% over a 20-min period. This response is correlated with the mitotic cell cycle but is not coupled to nuclear osmolytes released by nuclear envelope breakdown, chromatin condensation, or cytokinesis and does not result from endocytosis of the surrounding fluid. Inhibiting Na-H exchange eliminates the response. Although mitotic rounding of adherent cells is necessary for proper cell division, our observations that suspended cells undergo reversible swelling during mitosis suggest that regulation of intracellular water may be a more general component of mitosis than previously appreciated.
format Online
Article
Text
id pubmed-4657169
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-46571692016-05-23 Resonant microchannel volume and mass measurements show that suspended cells swell during mitosis Son, Sungmin Kang, Joon Ho Oh, Seungeun Kirschner, Marc W. Mitchison, T.J. Manalis, Scott J Cell Biol Research Articles Osmotic regulation of intracellular water during mitosis is poorly understood because methods for monitoring relevant cellular physical properties with sufficient precision have been limited. Here we use a suspended microchannel resonator to monitor the volume and density of single cells in suspension with a precision of 1% and 0.03%, respectively. We find that for transformed murine lymphocytic leukemia and mouse pro–B cell lymphoid cell lines, mitotic cells reversibly increase their volume by more than 10% and decrease their density by 0.4% over a 20-min period. This response is correlated with the mitotic cell cycle but is not coupled to nuclear osmolytes released by nuclear envelope breakdown, chromatin condensation, or cytokinesis and does not result from endocytosis of the surrounding fluid. Inhibiting Na-H exchange eliminates the response. Although mitotic rounding of adherent cells is necessary for proper cell division, our observations that suspended cells undergo reversible swelling during mitosis suggest that regulation of intracellular water may be a more general component of mitosis than previously appreciated. The Rockefeller University Press 2015-11-23 /pmc/articles/PMC4657169/ /pubmed/26598613 http://dx.doi.org/10.1083/jcb.201505058 Text en © 2015 Son et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Son, Sungmin
Kang, Joon Ho
Oh, Seungeun
Kirschner, Marc W.
Mitchison, T.J.
Manalis, Scott
Resonant microchannel volume and mass measurements show that suspended cells swell during mitosis
title Resonant microchannel volume and mass measurements show that suspended cells swell during mitosis
title_full Resonant microchannel volume and mass measurements show that suspended cells swell during mitosis
title_fullStr Resonant microchannel volume and mass measurements show that suspended cells swell during mitosis
title_full_unstemmed Resonant microchannel volume and mass measurements show that suspended cells swell during mitosis
title_short Resonant microchannel volume and mass measurements show that suspended cells swell during mitosis
title_sort resonant microchannel volume and mass measurements show that suspended cells swell during mitosis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4657169/
https://www.ncbi.nlm.nih.gov/pubmed/26598613
http://dx.doi.org/10.1083/jcb.201505058
work_keys_str_mv AT sonsungmin resonantmicrochannelvolumeandmassmeasurementsshowthatsuspendedcellsswellduringmitosis
AT kangjoonho resonantmicrochannelvolumeandmassmeasurementsshowthatsuspendedcellsswellduringmitosis
AT ohseungeun resonantmicrochannelvolumeandmassmeasurementsshowthatsuspendedcellsswellduringmitosis
AT kirschnermarcw resonantmicrochannelvolumeandmassmeasurementsshowthatsuspendedcellsswellduringmitosis
AT mitchisontj resonantmicrochannelvolumeandmassmeasurementsshowthatsuspendedcellsswellduringmitosis
AT manalisscott resonantmicrochannelvolumeandmassmeasurementsshowthatsuspendedcellsswellduringmitosis