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Sexual characteristics of high-temperature sterilized male Mozambique tilapia, Oreochromis mossambicus
INTRODUCTION: In order to clarify the effect of extremely high temperature on gonads of fish, juveniles of the Mozambique tilapia, Oreochromis mossambicus, at three days after hatching (d.a.h.) were reared at a high temperature (37 ± 0.5 °C) for 50 days. The heat-treated fish were then cultivated at...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4657368/ https://www.ncbi.nlm.nih.gov/pubmed/26605066 http://dx.doi.org/10.1186/s40851-015-0021-4 |
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author | Nakamura, Masaru Nozu, Ryo Ijiri, Shigeho Kobayashi, Tohru Hirai, Toshiaki Yamaguchi, Yoko Seale, Andre Lerner, Darren T. Grau, Gordon E. |
author_facet | Nakamura, Masaru Nozu, Ryo Ijiri, Shigeho Kobayashi, Tohru Hirai, Toshiaki Yamaguchi, Yoko Seale, Andre Lerner, Darren T. Grau, Gordon E. |
author_sort | Nakamura, Masaru |
collection | PubMed |
description | INTRODUCTION: In order to clarify the effect of extremely high temperature on gonads of fish, juveniles of the Mozambique tilapia, Oreochromis mossambicus, at three days after hatching (d.a.h.) were reared at a high temperature (37 ± 0.5 °C) for 50 days. The heat-treated fish were then cultivated at a normal water temperature for over six months. RESULTS: The testes of all individuals heat-treated for 50 days were sterile. Histological analysis revealed the complete absence of all stages of spermatogenic germ cells in the testes of the heat-treated males; however, structures within a layer of epithelial cells lining the efferent ducts were observed to actively secrete sperm fluid into the ducts, as in the mature testes of normal males. Clusters of cells immunopositive against P450scc and 3β-hydroxysteroid dehydrogenase were observed in the sterilized testes. Leydig cells had developed smooth endoplasmic reticulum and several mitochondria with tubular cristae indicating active steroidogenesis. The sterilized males displayed male nuptial coloration, actively dug spawning nests, and mated with normal mature females. However, females mated with these males initially brooded their eggs normally but released them prematurely at 4–5 days. All the released eggs were unfertilized and dead. CONCLUSION: Heat-sterilized male tilapia matures endocrinologically but completely lacks spermatogenic germ cells. |
format | Online Article Text |
id | pubmed-4657368 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-46573682015-11-24 Sexual characteristics of high-temperature sterilized male Mozambique tilapia, Oreochromis mossambicus Nakamura, Masaru Nozu, Ryo Ijiri, Shigeho Kobayashi, Tohru Hirai, Toshiaki Yamaguchi, Yoko Seale, Andre Lerner, Darren T. Grau, Gordon E. Zoological Lett Research Article INTRODUCTION: In order to clarify the effect of extremely high temperature on gonads of fish, juveniles of the Mozambique tilapia, Oreochromis mossambicus, at three days after hatching (d.a.h.) were reared at a high temperature (37 ± 0.5 °C) for 50 days. The heat-treated fish were then cultivated at a normal water temperature for over six months. RESULTS: The testes of all individuals heat-treated for 50 days were sterile. Histological analysis revealed the complete absence of all stages of spermatogenic germ cells in the testes of the heat-treated males; however, structures within a layer of epithelial cells lining the efferent ducts were observed to actively secrete sperm fluid into the ducts, as in the mature testes of normal males. Clusters of cells immunopositive against P450scc and 3β-hydroxysteroid dehydrogenase were observed in the sterilized testes. Leydig cells had developed smooth endoplasmic reticulum and several mitochondria with tubular cristae indicating active steroidogenesis. The sterilized males displayed male nuptial coloration, actively dug spawning nests, and mated with normal mature females. However, females mated with these males initially brooded their eggs normally but released them prematurely at 4–5 days. All the released eggs were unfertilized and dead. CONCLUSION: Heat-sterilized male tilapia matures endocrinologically but completely lacks spermatogenic germ cells. BioMed Central 2015-07-22 /pmc/articles/PMC4657368/ /pubmed/26605066 http://dx.doi.org/10.1186/s40851-015-0021-4 Text en © Nakamura et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Nakamura, Masaru Nozu, Ryo Ijiri, Shigeho Kobayashi, Tohru Hirai, Toshiaki Yamaguchi, Yoko Seale, Andre Lerner, Darren T. Grau, Gordon E. Sexual characteristics of high-temperature sterilized male Mozambique tilapia, Oreochromis mossambicus |
title | Sexual characteristics of high-temperature sterilized male Mozambique tilapia, Oreochromis mossambicus |
title_full | Sexual characteristics of high-temperature sterilized male Mozambique tilapia, Oreochromis mossambicus |
title_fullStr | Sexual characteristics of high-temperature sterilized male Mozambique tilapia, Oreochromis mossambicus |
title_full_unstemmed | Sexual characteristics of high-temperature sterilized male Mozambique tilapia, Oreochromis mossambicus |
title_short | Sexual characteristics of high-temperature sterilized male Mozambique tilapia, Oreochromis mossambicus |
title_sort | sexual characteristics of high-temperature sterilized male mozambique tilapia, oreochromis mossambicus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4657368/ https://www.ncbi.nlm.nih.gov/pubmed/26605066 http://dx.doi.org/10.1186/s40851-015-0021-4 |
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