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Genetic Susceptibility to Cardiac and Digestive Clinical Forms of Chronic Chagas Disease: Involvement of the CCR5 59029 A/G Polymorphism
The clinical manifestations of chronic Chagas disease include the cardiac form of the disease and the digestive form. Not all the factors that act in the variable clinical course of this disease are known. This study investigated whether the CCR5Δ32 (rs333) and CCR5 59029 A/G (promoter region—rs1799...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4657911/ https://www.ncbi.nlm.nih.gov/pubmed/26599761 http://dx.doi.org/10.1371/journal.pone.0141847 |
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author | de Oliveira, Amanda Priscila Bernardo, Cássia Rubia Camargo, Ana Vitória da Silveira Ronchi, Luiz Sérgio Borim, Aldenis Albaneze Brandão de Mattos, Cinara Cássia de Campos Júnior, Eumildo Castiglioni, Lílian Netinho, João Gomes Cavasini, Carlos Eugênio Bestetti, Reinaldo Bulgarelli de Mattos, Luiz Carlos |
author_facet | de Oliveira, Amanda Priscila Bernardo, Cássia Rubia Camargo, Ana Vitória da Silveira Ronchi, Luiz Sérgio Borim, Aldenis Albaneze Brandão de Mattos, Cinara Cássia de Campos Júnior, Eumildo Castiglioni, Lílian Netinho, João Gomes Cavasini, Carlos Eugênio Bestetti, Reinaldo Bulgarelli de Mattos, Luiz Carlos |
author_sort | de Oliveira, Amanda Priscila |
collection | PubMed |
description | The clinical manifestations of chronic Chagas disease include the cardiac form of the disease and the digestive form. Not all the factors that act in the variable clinical course of this disease are known. This study investigated whether the CCR5Δ32 (rs333) and CCR5 59029 A/G (promoter region—rs1799987) polymorphisms of the CCR5 gene are associated with different clinical forms of chronic Chagas disease and with the severity of left ventricular systolic dysfunction in patients with chronic Chagas heart disease (CCHD). The antibodies anti-T. cruzi were identified by ELISA. PCR and PCR-RFLP were used to identify the CCR5Δ32 and CCR5 59029 A/G polymorphisms. The chi-square test was used to compare variables between groups. There was a higher frequency of the AA genotype in patients with CCHD compared with patients with the digestive form of the disease and the control group. The results also showed a high frequency of the AG genotype in patients with the digestive form of the disease compared to the other groups. The results of this study show that the CCR5Δ32 polymorphism does not seem to influence the different clinical manifestations of Chagas disease but there is involvement of the CCR5 59029 A/G polymorphism in susceptibility to the different forms of chronic Chagas disease. Besides, these polymorphisms do not influence left ventricular systolic dysfunction in patients with CCHD. |
format | Online Article Text |
id | pubmed-4657911 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-46579112015-12-02 Genetic Susceptibility to Cardiac and Digestive Clinical Forms of Chronic Chagas Disease: Involvement of the CCR5 59029 A/G Polymorphism de Oliveira, Amanda Priscila Bernardo, Cássia Rubia Camargo, Ana Vitória da Silveira Ronchi, Luiz Sérgio Borim, Aldenis Albaneze Brandão de Mattos, Cinara Cássia de Campos Júnior, Eumildo Castiglioni, Lílian Netinho, João Gomes Cavasini, Carlos Eugênio Bestetti, Reinaldo Bulgarelli de Mattos, Luiz Carlos PLoS One Research Article The clinical manifestations of chronic Chagas disease include the cardiac form of the disease and the digestive form. Not all the factors that act in the variable clinical course of this disease are known. This study investigated whether the CCR5Δ32 (rs333) and CCR5 59029 A/G (promoter region—rs1799987) polymorphisms of the CCR5 gene are associated with different clinical forms of chronic Chagas disease and with the severity of left ventricular systolic dysfunction in patients with chronic Chagas heart disease (CCHD). The antibodies anti-T. cruzi were identified by ELISA. PCR and PCR-RFLP were used to identify the CCR5Δ32 and CCR5 59029 A/G polymorphisms. The chi-square test was used to compare variables between groups. There was a higher frequency of the AA genotype in patients with CCHD compared with patients with the digestive form of the disease and the control group. The results also showed a high frequency of the AG genotype in patients with the digestive form of the disease compared to the other groups. The results of this study show that the CCR5Δ32 polymorphism does not seem to influence the different clinical manifestations of Chagas disease but there is involvement of the CCR5 59029 A/G polymorphism in susceptibility to the different forms of chronic Chagas disease. Besides, these polymorphisms do not influence left ventricular systolic dysfunction in patients with CCHD. Public Library of Science 2015-11-23 /pmc/articles/PMC4657911/ /pubmed/26599761 http://dx.doi.org/10.1371/journal.pone.0141847 Text en © 2015 de Oliveira et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article de Oliveira, Amanda Priscila Bernardo, Cássia Rubia Camargo, Ana Vitória da Silveira Ronchi, Luiz Sérgio Borim, Aldenis Albaneze Brandão de Mattos, Cinara Cássia de Campos Júnior, Eumildo Castiglioni, Lílian Netinho, João Gomes Cavasini, Carlos Eugênio Bestetti, Reinaldo Bulgarelli de Mattos, Luiz Carlos Genetic Susceptibility to Cardiac and Digestive Clinical Forms of Chronic Chagas Disease: Involvement of the CCR5 59029 A/G Polymorphism |
title | Genetic Susceptibility to Cardiac and Digestive Clinical Forms of Chronic Chagas Disease: Involvement of the CCR5 59029 A/G Polymorphism |
title_full | Genetic Susceptibility to Cardiac and Digestive Clinical Forms of Chronic Chagas Disease: Involvement of the CCR5 59029 A/G Polymorphism |
title_fullStr | Genetic Susceptibility to Cardiac and Digestive Clinical Forms of Chronic Chagas Disease: Involvement of the CCR5 59029 A/G Polymorphism |
title_full_unstemmed | Genetic Susceptibility to Cardiac and Digestive Clinical Forms of Chronic Chagas Disease: Involvement of the CCR5 59029 A/G Polymorphism |
title_short | Genetic Susceptibility to Cardiac and Digestive Clinical Forms of Chronic Chagas Disease: Involvement of the CCR5 59029 A/G Polymorphism |
title_sort | genetic susceptibility to cardiac and digestive clinical forms of chronic chagas disease: involvement of the ccr5 59029 a/g polymorphism |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4657911/ https://www.ncbi.nlm.nih.gov/pubmed/26599761 http://dx.doi.org/10.1371/journal.pone.0141847 |
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