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Reduction of Adipose Tissue Mass by the Angiogenesis Inhibitor ALS-L1023 from Melissa officinalis

It has been suggested that angiogenesis modulates adipogenesis and obesity. This study was undertaken to determine whether ALS-L1023 (ALS) prepared by a two-step organic solvent fractionation from Melissa leaves, which exhibits antiangiogenic activity, can regulate adipose tissue growth. The effects...

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Autores principales: Park, Byung Young, Lee, Hyunghee, Woo, Sangee, Yoon, Miso, Kim, Jeongjun, Hong, Yeonhee, Lee, Hee Suk, Park, Eun Kyu, Hahm, Jong Cheon, Kim, Jin Woo, Shin, Soon Shik, Kim, Min-Young, Yoon, Michung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4658083/
https://www.ncbi.nlm.nih.gov/pubmed/26599360
http://dx.doi.org/10.1371/journal.pone.0141612
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author Park, Byung Young
Lee, Hyunghee
Woo, Sangee
Yoon, Miso
Kim, Jeongjun
Hong, Yeonhee
Lee, Hee Suk
Park, Eun Kyu
Hahm, Jong Cheon
Kim, Jin Woo
Shin, Soon Shik
Kim, Min-Young
Yoon, Michung
author_facet Park, Byung Young
Lee, Hyunghee
Woo, Sangee
Yoon, Miso
Kim, Jeongjun
Hong, Yeonhee
Lee, Hee Suk
Park, Eun Kyu
Hahm, Jong Cheon
Kim, Jin Woo
Shin, Soon Shik
Kim, Min-Young
Yoon, Michung
author_sort Park, Byung Young
collection PubMed
description It has been suggested that angiogenesis modulates adipogenesis and obesity. This study was undertaken to determine whether ALS-L1023 (ALS) prepared by a two-step organic solvent fractionation from Melissa leaves, which exhibits antiangiogenic activity, can regulate adipose tissue growth. The effects of ALS on angiogenesis and extracellular matrix remodeling were measured using in vitro assays. The effects of ALS on adipose tissue growth were investigated in high fat diet-induced obese mice. ALS inhibited VEGF- and bFGF-induced endothelial cell proliferation and suppressed matrix metalloproteinase (MMP) activity in vitro. Compared to obese control mice, administration of ALS to obese mice reduced body weight gain, adipose tissue mass and adipocyte size without affecting appetite. ALS treatment decreased blood vessel density and MMP activity in adipose tissues. ALS reduced the mRNA levels of angiogenic factors (VEGF-A and FGF-2) and MMPs (MMP-2 and MMP-9), whereas ALS increased the mRNA levels of angiogenic inhibitors (TSP-1, TIMP-1, and TIMP-2) in adipose tissues. The protein levels of VEGF, MMP-2 and MMP-9 were also decreased by ALS in adipose tissue. Metabolic changes in plasma lipids, liver triglycerides, and hepatic expression of fatty acid oxidation genes occurred during ALS-induced weight loss. These results suggest that ALS, which has antiangiogenic and MMP inhibitory activities, reduces adipose tissue mass in nutritionally obese mice, demonstrating that adipose tissue growth can be regulated by angiogenesis inhibitors.
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spelling pubmed-46580832015-12-02 Reduction of Adipose Tissue Mass by the Angiogenesis Inhibitor ALS-L1023 from Melissa officinalis Park, Byung Young Lee, Hyunghee Woo, Sangee Yoon, Miso Kim, Jeongjun Hong, Yeonhee Lee, Hee Suk Park, Eun Kyu Hahm, Jong Cheon Kim, Jin Woo Shin, Soon Shik Kim, Min-Young Yoon, Michung PLoS One Research Article It has been suggested that angiogenesis modulates adipogenesis and obesity. This study was undertaken to determine whether ALS-L1023 (ALS) prepared by a two-step organic solvent fractionation from Melissa leaves, which exhibits antiangiogenic activity, can regulate adipose tissue growth. The effects of ALS on angiogenesis and extracellular matrix remodeling were measured using in vitro assays. The effects of ALS on adipose tissue growth were investigated in high fat diet-induced obese mice. ALS inhibited VEGF- and bFGF-induced endothelial cell proliferation and suppressed matrix metalloproteinase (MMP) activity in vitro. Compared to obese control mice, administration of ALS to obese mice reduced body weight gain, adipose tissue mass and adipocyte size without affecting appetite. ALS treatment decreased blood vessel density and MMP activity in adipose tissues. ALS reduced the mRNA levels of angiogenic factors (VEGF-A and FGF-2) and MMPs (MMP-2 and MMP-9), whereas ALS increased the mRNA levels of angiogenic inhibitors (TSP-1, TIMP-1, and TIMP-2) in adipose tissues. The protein levels of VEGF, MMP-2 and MMP-9 were also decreased by ALS in adipose tissue. Metabolic changes in plasma lipids, liver triglycerides, and hepatic expression of fatty acid oxidation genes occurred during ALS-induced weight loss. These results suggest that ALS, which has antiangiogenic and MMP inhibitory activities, reduces adipose tissue mass in nutritionally obese mice, demonstrating that adipose tissue growth can be regulated by angiogenesis inhibitors. Public Library of Science 2015-11-23 /pmc/articles/PMC4658083/ /pubmed/26599360 http://dx.doi.org/10.1371/journal.pone.0141612 Text en © 2015 Park et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Park, Byung Young
Lee, Hyunghee
Woo, Sangee
Yoon, Miso
Kim, Jeongjun
Hong, Yeonhee
Lee, Hee Suk
Park, Eun Kyu
Hahm, Jong Cheon
Kim, Jin Woo
Shin, Soon Shik
Kim, Min-Young
Yoon, Michung
Reduction of Adipose Tissue Mass by the Angiogenesis Inhibitor ALS-L1023 from Melissa officinalis
title Reduction of Adipose Tissue Mass by the Angiogenesis Inhibitor ALS-L1023 from Melissa officinalis
title_full Reduction of Adipose Tissue Mass by the Angiogenesis Inhibitor ALS-L1023 from Melissa officinalis
title_fullStr Reduction of Adipose Tissue Mass by the Angiogenesis Inhibitor ALS-L1023 from Melissa officinalis
title_full_unstemmed Reduction of Adipose Tissue Mass by the Angiogenesis Inhibitor ALS-L1023 from Melissa officinalis
title_short Reduction of Adipose Tissue Mass by the Angiogenesis Inhibitor ALS-L1023 from Melissa officinalis
title_sort reduction of adipose tissue mass by the angiogenesis inhibitor als-l1023 from melissa officinalis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4658083/
https://www.ncbi.nlm.nih.gov/pubmed/26599360
http://dx.doi.org/10.1371/journal.pone.0141612
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