Cargando…

Phytochemical Constituents, Antioxidant and Antiproliferative Properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia

The study aimed to investigate the phytochemical contents, antioxidant and antiproliferative activity of 80% methanol extract of Lepidozia borneensis. The total phenolic and total flavonoid contents were analysed using Folin-Ciocalteu and aluminium chloride colorimetric methods. Antioxidant properti...

Descripción completa

Detalles Bibliográficos
Autores principales: Abu Bakar, Mohd Fadzelly, Abdul Karim, Fifilyana, Suleiman, Monica, Isha, Azizul, Rahmat, Asmah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4658452/
https://www.ncbi.nlm.nih.gov/pubmed/26640502
http://dx.doi.org/10.1155/2015/936215
_version_ 1782402518159982592
author Abu Bakar, Mohd Fadzelly
Abdul Karim, Fifilyana
Suleiman, Monica
Isha, Azizul
Rahmat, Asmah
author_facet Abu Bakar, Mohd Fadzelly
Abdul Karim, Fifilyana
Suleiman, Monica
Isha, Azizul
Rahmat, Asmah
author_sort Abu Bakar, Mohd Fadzelly
collection PubMed
description The study aimed to investigate the phytochemical contents, antioxidant and antiproliferative activity of 80% methanol extract of Lepidozia borneensis. The total phenolic and total flavonoid contents were analysed using Folin-Ciocalteu and aluminium chloride colorimetric methods. Antioxidant properties were evaluated by using FRAP, ABTS, and DPPH assays while the effects of L. borneensis on the proliferation of MCF-7 cell line were evaluated by using MTT assay. The results showed that the total phenolic and flavonoid contents were 12.42 ± 0.47 mg GAE/g and 9.36 ± 1.29 mg CE/g, respectively. The GC-MS analysis revealed the presence of at least 35 compounds. The extract was found to induce cytotoxicity against MCF-7 cell line with IC(50) value of 47.33 ± 7.37 µg/mL. Cell cycle analysis showed that the extract induced significant arrest at G(0)/G(1) at 24 hours of treatment. After 72 hours of treatment, the proportion of cells in G(0)/G(1) and G(2)-M phases had decreased significantly as compared to their control. Apoptosis occurred during the first 24 hours and significantly increased to 30.8% after 72 hours of treatment. No activation of caspase 3 was observed. These findings suggest that L. borneensis extract has the potential as natural antioxidant and anticancer agents.
format Online
Article
Text
id pubmed-4658452
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-46584522015-12-06 Phytochemical Constituents, Antioxidant and Antiproliferative Properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia Abu Bakar, Mohd Fadzelly Abdul Karim, Fifilyana Suleiman, Monica Isha, Azizul Rahmat, Asmah Evid Based Complement Alternat Med Research Article The study aimed to investigate the phytochemical contents, antioxidant and antiproliferative activity of 80% methanol extract of Lepidozia borneensis. The total phenolic and total flavonoid contents were analysed using Folin-Ciocalteu and aluminium chloride colorimetric methods. Antioxidant properties were evaluated by using FRAP, ABTS, and DPPH assays while the effects of L. borneensis on the proliferation of MCF-7 cell line were evaluated by using MTT assay. The results showed that the total phenolic and flavonoid contents were 12.42 ± 0.47 mg GAE/g and 9.36 ± 1.29 mg CE/g, respectively. The GC-MS analysis revealed the presence of at least 35 compounds. The extract was found to induce cytotoxicity against MCF-7 cell line with IC(50) value of 47.33 ± 7.37 µg/mL. Cell cycle analysis showed that the extract induced significant arrest at G(0)/G(1) at 24 hours of treatment. After 72 hours of treatment, the proportion of cells in G(0)/G(1) and G(2)-M phases had decreased significantly as compared to their control. Apoptosis occurred during the first 24 hours and significantly increased to 30.8% after 72 hours of treatment. No activation of caspase 3 was observed. These findings suggest that L. borneensis extract has the potential as natural antioxidant and anticancer agents. Hindawi Publishing Corporation 2015 2015-11-11 /pmc/articles/PMC4658452/ /pubmed/26640502 http://dx.doi.org/10.1155/2015/936215 Text en Copyright © 2015 Mohd Fadzelly Abu Bakar et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Abu Bakar, Mohd Fadzelly
Abdul Karim, Fifilyana
Suleiman, Monica
Isha, Azizul
Rahmat, Asmah
Phytochemical Constituents, Antioxidant and Antiproliferative Properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia
title Phytochemical Constituents, Antioxidant and Antiproliferative Properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia
title_full Phytochemical Constituents, Antioxidant and Antiproliferative Properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia
title_fullStr Phytochemical Constituents, Antioxidant and Antiproliferative Properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia
title_full_unstemmed Phytochemical Constituents, Antioxidant and Antiproliferative Properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia
title_short Phytochemical Constituents, Antioxidant and Antiproliferative Properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia
title_sort phytochemical constituents, antioxidant and antiproliferative properties of a liverwort, lepidozia borneensis stephani from mount kinabalu, sabah, malaysia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4658452/
https://www.ncbi.nlm.nih.gov/pubmed/26640502
http://dx.doi.org/10.1155/2015/936215
work_keys_str_mv AT abubakarmohdfadzelly phytochemicalconstituentsantioxidantandantiproliferativepropertiesofaliverwortlepidoziaborneensisstephanifrommountkinabalusabahmalaysia
AT abdulkarimfifilyana phytochemicalconstituentsantioxidantandantiproliferativepropertiesofaliverwortlepidoziaborneensisstephanifrommountkinabalusabahmalaysia
AT suleimanmonica phytochemicalconstituentsantioxidantandantiproliferativepropertiesofaliverwortlepidoziaborneensisstephanifrommountkinabalusabahmalaysia
AT ishaazizul phytochemicalconstituentsantioxidantandantiproliferativepropertiesofaliverwortlepidoziaborneensisstephanifrommountkinabalusabahmalaysia
AT rahmatasmah phytochemicalconstituentsantioxidantandantiproliferativepropertiesofaliverwortlepidoziaborneensisstephanifrommountkinabalusabahmalaysia