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Phytochemical Constituents, Antioxidant and Antiproliferative Properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia
The study aimed to investigate the phytochemical contents, antioxidant and antiproliferative activity of 80% methanol extract of Lepidozia borneensis. The total phenolic and total flavonoid contents were analysed using Folin-Ciocalteu and aluminium chloride colorimetric methods. Antioxidant properti...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4658452/ https://www.ncbi.nlm.nih.gov/pubmed/26640502 http://dx.doi.org/10.1155/2015/936215 |
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author | Abu Bakar, Mohd Fadzelly Abdul Karim, Fifilyana Suleiman, Monica Isha, Azizul Rahmat, Asmah |
author_facet | Abu Bakar, Mohd Fadzelly Abdul Karim, Fifilyana Suleiman, Monica Isha, Azizul Rahmat, Asmah |
author_sort | Abu Bakar, Mohd Fadzelly |
collection | PubMed |
description | The study aimed to investigate the phytochemical contents, antioxidant and antiproliferative activity of 80% methanol extract of Lepidozia borneensis. The total phenolic and total flavonoid contents were analysed using Folin-Ciocalteu and aluminium chloride colorimetric methods. Antioxidant properties were evaluated by using FRAP, ABTS, and DPPH assays while the effects of L. borneensis on the proliferation of MCF-7 cell line were evaluated by using MTT assay. The results showed that the total phenolic and flavonoid contents were 12.42 ± 0.47 mg GAE/g and 9.36 ± 1.29 mg CE/g, respectively. The GC-MS analysis revealed the presence of at least 35 compounds. The extract was found to induce cytotoxicity against MCF-7 cell line with IC(50) value of 47.33 ± 7.37 µg/mL. Cell cycle analysis showed that the extract induced significant arrest at G(0)/G(1) at 24 hours of treatment. After 72 hours of treatment, the proportion of cells in G(0)/G(1) and G(2)-M phases had decreased significantly as compared to their control. Apoptosis occurred during the first 24 hours and significantly increased to 30.8% after 72 hours of treatment. No activation of caspase 3 was observed. These findings suggest that L. borneensis extract has the potential as natural antioxidant and anticancer agents. |
format | Online Article Text |
id | pubmed-4658452 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-46584522015-12-06 Phytochemical Constituents, Antioxidant and Antiproliferative Properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia Abu Bakar, Mohd Fadzelly Abdul Karim, Fifilyana Suleiman, Monica Isha, Azizul Rahmat, Asmah Evid Based Complement Alternat Med Research Article The study aimed to investigate the phytochemical contents, antioxidant and antiproliferative activity of 80% methanol extract of Lepidozia borneensis. The total phenolic and total flavonoid contents were analysed using Folin-Ciocalteu and aluminium chloride colorimetric methods. Antioxidant properties were evaluated by using FRAP, ABTS, and DPPH assays while the effects of L. borneensis on the proliferation of MCF-7 cell line were evaluated by using MTT assay. The results showed that the total phenolic and flavonoid contents were 12.42 ± 0.47 mg GAE/g and 9.36 ± 1.29 mg CE/g, respectively. The GC-MS analysis revealed the presence of at least 35 compounds. The extract was found to induce cytotoxicity against MCF-7 cell line with IC(50) value of 47.33 ± 7.37 µg/mL. Cell cycle analysis showed that the extract induced significant arrest at G(0)/G(1) at 24 hours of treatment. After 72 hours of treatment, the proportion of cells in G(0)/G(1) and G(2)-M phases had decreased significantly as compared to their control. Apoptosis occurred during the first 24 hours and significantly increased to 30.8% after 72 hours of treatment. No activation of caspase 3 was observed. These findings suggest that L. borneensis extract has the potential as natural antioxidant and anticancer agents. Hindawi Publishing Corporation 2015 2015-11-11 /pmc/articles/PMC4658452/ /pubmed/26640502 http://dx.doi.org/10.1155/2015/936215 Text en Copyright © 2015 Mohd Fadzelly Abu Bakar et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Abu Bakar, Mohd Fadzelly Abdul Karim, Fifilyana Suleiman, Monica Isha, Azizul Rahmat, Asmah Phytochemical Constituents, Antioxidant and Antiproliferative Properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia |
title | Phytochemical Constituents, Antioxidant and Antiproliferative Properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia |
title_full | Phytochemical Constituents, Antioxidant and Antiproliferative Properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia |
title_fullStr | Phytochemical Constituents, Antioxidant and Antiproliferative Properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia |
title_full_unstemmed | Phytochemical Constituents, Antioxidant and Antiproliferative Properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia |
title_short | Phytochemical Constituents, Antioxidant and Antiproliferative Properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia |
title_sort | phytochemical constituents, antioxidant and antiproliferative properties of a liverwort, lepidozia borneensis stephani from mount kinabalu, sabah, malaysia |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4658452/ https://www.ncbi.nlm.nih.gov/pubmed/26640502 http://dx.doi.org/10.1155/2015/936215 |
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