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Evaluation of chemopreventive potential of Strobilanthes crispus against colon cancer formation in vitro and in vivo

BACKGROUND: With cancer being one of the major causes of death around the world, studies are ongoing to find new chemotherapeutic leads. There are common mechanisms for colorectal cancer (CRC) formation. Several are connected with oxidative stress-induced cell apoptosis and others are related to imb...

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Autores principales: Al-Henhena, Nawal, Khalifa, Shaden A. M., Ying, Rozaida Poh Yuen, Ismail, Salmah, Hamadi, Riad, Shawter, Abdrabu N., Idris, Azila Mohd, Azizan, Ainnul, Al-Wajeeh, Nahla Saeed, Abdulla, Mahmood Ameen, El-Seedi, Hesham R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4658747/
https://www.ncbi.nlm.nih.gov/pubmed/26608653
http://dx.doi.org/10.1186/s12906-015-0926-7
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author Al-Henhena, Nawal
Khalifa, Shaden A. M.
Ying, Rozaida Poh Yuen
Ismail, Salmah
Hamadi, Riad
Shawter, Abdrabu N.
Idris, Azila Mohd
Azizan, Ainnul
Al-Wajeeh, Nahla Saeed
Abdulla, Mahmood Ameen
El-Seedi, Hesham R.
author_facet Al-Henhena, Nawal
Khalifa, Shaden A. M.
Ying, Rozaida Poh Yuen
Ismail, Salmah
Hamadi, Riad
Shawter, Abdrabu N.
Idris, Azila Mohd
Azizan, Ainnul
Al-Wajeeh, Nahla Saeed
Abdulla, Mahmood Ameen
El-Seedi, Hesham R.
author_sort Al-Henhena, Nawal
collection PubMed
description BACKGROUND: With cancer being one of the major causes of death around the world, studies are ongoing to find new chemotherapeutic leads. There are common mechanisms for colorectal cancer (CRC) formation. Several are connected with oxidative stress-induced cell apoptosis and others are related to imbalanced homeostasis or intake of drugs/toxins. Plants that have been used for decades in folk and traditional medicine have been accepted as one of the commonest sources of discovered natural agents of cancer chemotherapy and chemoprevention. The aim was to study the antioxidant and chemopreventive effects of Strobilanthes crispus on colorectal cancer formation. METHODS: Five groups of rats were injected subcutaneously with AOM, 15 mg/kg body weight, each once weekly for 2 weeks. The cancer group was continued on 10 % Tween-20 feeding for 8 weeks. The standard drug group was continued on 35 mg/kg 5-fluorouracil intraperitoneal injection twice a week for 8 weeks, and the experimental groups were continued on 250 and 500 mg/kg S. crispus extract oral feeding for 8 weeks, respectively. The normal group was injected subcutaneously with normal saline once a week for 2 weeks, followed by oral administration of 10 % Tween-20 for 8 weeks. All the rats were sacrificed after 10 weeks. The colons were evaluated grossly and histopathologically for aberrant crypt foci (ACF). Gene expression was performed for Bax, Bcl2, Defa24, Slc24a3, and APC genes by real-time PCR. S. crispus and its fractions were evaluated for their chemopreventive effects against human colorectal adenocarcinoma cell line HT29 and cytotoxicity for normal human colon epithelial cell line CCD 841, and the active fraction was assessed for its components. RESULTS: We observed significant decrease in total colonic ACF formation, malonaldehyde (MDA) and lactate dehydrogenase (LDH), increase in superoxide dismutase (SOD), up-regulation of APC, Bax and Slc24a3, and down-regulation of Defa24 and Bcl-2 in rats treated with Strobilanthes crispus. CONCLUSION: Our results support the in vivo protection of S. crispus against CRC formation (azoxymethane-induced aberrant crypt foci) and suggest that the mechanism is highly specific to protect from oxidative insults and the following apoptotic cascade. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12906-015-0926-7) contains supplementary material, which is available to authorized users.
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spelling pubmed-46587472015-11-26 Evaluation of chemopreventive potential of Strobilanthes crispus against colon cancer formation in vitro and in vivo Al-Henhena, Nawal Khalifa, Shaden A. M. Ying, Rozaida Poh Yuen Ismail, Salmah Hamadi, Riad Shawter, Abdrabu N. Idris, Azila Mohd Azizan, Ainnul Al-Wajeeh, Nahla Saeed Abdulla, Mahmood Ameen El-Seedi, Hesham R. BMC Complement Altern Med Research Article BACKGROUND: With cancer being one of the major causes of death around the world, studies are ongoing to find new chemotherapeutic leads. There are common mechanisms for colorectal cancer (CRC) formation. Several are connected with oxidative stress-induced cell apoptosis and others are related to imbalanced homeostasis or intake of drugs/toxins. Plants that have been used for decades in folk and traditional medicine have been accepted as one of the commonest sources of discovered natural agents of cancer chemotherapy and chemoprevention. The aim was to study the antioxidant and chemopreventive effects of Strobilanthes crispus on colorectal cancer formation. METHODS: Five groups of rats were injected subcutaneously with AOM, 15 mg/kg body weight, each once weekly for 2 weeks. The cancer group was continued on 10 % Tween-20 feeding for 8 weeks. The standard drug group was continued on 35 mg/kg 5-fluorouracil intraperitoneal injection twice a week for 8 weeks, and the experimental groups were continued on 250 and 500 mg/kg S. crispus extract oral feeding for 8 weeks, respectively. The normal group was injected subcutaneously with normal saline once a week for 2 weeks, followed by oral administration of 10 % Tween-20 for 8 weeks. All the rats were sacrificed after 10 weeks. The colons were evaluated grossly and histopathologically for aberrant crypt foci (ACF). Gene expression was performed for Bax, Bcl2, Defa24, Slc24a3, and APC genes by real-time PCR. S. crispus and its fractions were evaluated for their chemopreventive effects against human colorectal adenocarcinoma cell line HT29 and cytotoxicity for normal human colon epithelial cell line CCD 841, and the active fraction was assessed for its components. RESULTS: We observed significant decrease in total colonic ACF formation, malonaldehyde (MDA) and lactate dehydrogenase (LDH), increase in superoxide dismutase (SOD), up-regulation of APC, Bax and Slc24a3, and down-regulation of Defa24 and Bcl-2 in rats treated with Strobilanthes crispus. CONCLUSION: Our results support the in vivo protection of S. crispus against CRC formation (azoxymethane-induced aberrant crypt foci) and suggest that the mechanism is highly specific to protect from oxidative insults and the following apoptotic cascade. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12906-015-0926-7) contains supplementary material, which is available to authorized users. BioMed Central 2015-11-25 /pmc/articles/PMC4658747/ /pubmed/26608653 http://dx.doi.org/10.1186/s12906-015-0926-7 Text en © Al-Henhena et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Al-Henhena, Nawal
Khalifa, Shaden A. M.
Ying, Rozaida Poh Yuen
Ismail, Salmah
Hamadi, Riad
Shawter, Abdrabu N.
Idris, Azila Mohd
Azizan, Ainnul
Al-Wajeeh, Nahla Saeed
Abdulla, Mahmood Ameen
El-Seedi, Hesham R.
Evaluation of chemopreventive potential of Strobilanthes crispus against colon cancer formation in vitro and in vivo
title Evaluation of chemopreventive potential of Strobilanthes crispus against colon cancer formation in vitro and in vivo
title_full Evaluation of chemopreventive potential of Strobilanthes crispus against colon cancer formation in vitro and in vivo
title_fullStr Evaluation of chemopreventive potential of Strobilanthes crispus against colon cancer formation in vitro and in vivo
title_full_unstemmed Evaluation of chemopreventive potential of Strobilanthes crispus against colon cancer formation in vitro and in vivo
title_short Evaluation of chemopreventive potential of Strobilanthes crispus against colon cancer formation in vitro and in vivo
title_sort evaluation of chemopreventive potential of strobilanthes crispus against colon cancer formation in vitro and in vivo
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4658747/
https://www.ncbi.nlm.nih.gov/pubmed/26608653
http://dx.doi.org/10.1186/s12906-015-0926-7
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