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The use of a viral 2A sequence for the simultaneous over-expression of both the vgf gene and enhanced green fluorescent protein (eGFP) in vitro and in vivo
INTRODUCTION: The viral 2A sequence has become an attractive alternative to the traditional internal ribosomal entry site (IRES) for simultaneous over-expression of two genes and in combination with recombinant adeno-associated viruses (rAAV) has been used to manipulate gene expression in vitro. NEW...
Autores principales: | Lewis, Jo E., Brameld, John M., Hill, Phil, Barrett, Perry, Ebling, Francis J.P., Jethwa, Preeti H. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier/North-Holland Biomedical Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4659456/ https://www.ncbi.nlm.nih.gov/pubmed/26300182 http://dx.doi.org/10.1016/j.jneumeth.2015.08.013 |
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